AKTIVITAS ANTIFOTOOKSIDASI DAN PENGHAMBAT PEMBENTUKAN AGES (ADVANCED GLYCATION END-PRODUCTS) DARI FRAKSI ALGA Padina australis
DOI:
https://doi.org/10.35799/pha.7.2018.21422Abstract
AKTIVITAS ANTIFOTOOKSIDASI DAN PENGHAMBAT PEMBENTUKAN AGES (ADVANCED GLYCATION END-PRODUCTS) DARI FRAKSI ALGA Padina australis
Syutria Trifosa Kondoj1), Edi Suryanto2), Max Runtuwene2)
1)Program Studi Farmasi FMIPA Unsrat Manado, 95115
2)Jurusan Kimia FMIPA Unsrat Manado, 95115
ABSTRACT
This study aims to assess the anti photooxidation activity and inhibition of AGEs (Advanced Glycation End-Products) activity from fraction of Padina australis algae. This research has started by extracting powder Padina australis algae with maseration method using 75% methanol solvent. Futhermore, extract has fractionated by conventional column chromatography with the solvent of methanol and aquades. The fractions have tested by determine the total phenolic content, antioxidant, anti photooxidation and inhibitory of AGEs (Advanced Glycation End-Products) activity. The results show that the fraction of F4 has the highest phenolic total content, antioxidant, anti photooxidation, and inhibition of AGEs activity followed by the fractions of F2, F1, F3, and F5. The inhibition of AGEs (Advanced Glycation End-Products) activity in each fraction was analyzed by using UV-Vis spectrophotometer. Based on this research, it can be concluded that F4 has anti photooxidation activity and inhibiting of AGEs (Advanced Glycation End-Products) activity.
Keywords: Algae, Padina australis, anti photooxidation, AGEs in vitro
ABSTRAK
Penelitian ini bertujuan untuk mempelajari aktitivitas antifotooksidasi dan penghambat pembentukan AGEs (Advanced Glycation End-Products) dari Fraksi Alga Padina australis. Penelitian ini dimulai dengan mengekstraksi serbuk alga Padina australis menggunakan metode maserasi dengan pelarut metanol 75%. Kemudian, ekstrak difraksinasi menggunakan kromatografi kolom konvensional dengan pelarut metanol dan aquades. Hasil fraksi dikelompokkan menjadi 5 fraksi berdasarkan perbedaan warna. Fraksi-fraksi tersebut dilakukan pengujian untuk menentukan kandungan total fenolik, aktivitas antioksidan, antifotooksidasi, serta aktivitas penghambat pembentukan AGEs (Advanced Glycation End-Products). Hasilnya menunjukkan bahwa fraksi F4 memiliki kandungan total fenolik, aktivitas antioksidan, antifotooksidasi, dan penghambat pembentukan AGEs tertinggi diikuti F2, F3, F1, dan F5. Aktivitas penghambat pembentukan AGEs (Advanced Glycation End-Products) pada masing-masing fraksi dianalisis dengan menggunakan spektrofotometer UV-Vis. Berdasarkan penelitian ini, dapat disimpulkan bahwa F4 memiliki aktivitas antifotooksidasi dan penghambat pembentukan AGEs (Advanced Glycation End-Products).
Kata Kunci : Alga, Padina australis, antifotooksidasi, AGEs in vitro
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
