Screening antibacterial activity ODS fractions of marine sponges against non-pathogenic bacteria tuberculosis Mycobacterium smegmatis

Authors

  • Deiske A. Sumilat Sam Ratulangi University

DOI:

https://doi.org/10.35800/jasm.9.1.2021.32692

Keywords:

sponges, antibacterial, tuberculosis, Mycobacterium smegmatis, ODS

Abstract

Nine unidentified marine sponges from Bunaken Island waters in Manado Indonesia were observed on anti-tuberculosis (TB) against non-pathogenic Mycobacterium smegmatis NBRC 3207 and it was found that the ethanol crude extracts and ODS fractions of these sponges inhibited the growth of bacteria M. smegmatis. Sponge SP.323 is the most inhibiting and showed Intermediate (I) activity with inhibition zones of 9, 11, 11, 8 mm/disk at 10µg/disk., for fraction 3,4,5,6 respectively, and need further experimental analysis to justify the antimicrobial activity, NMR analysis and structure elucidation.

Author Biography

Deiske A. Sumilat, Sam Ratulangi University

Faculty of Fisheries and Marine Science, Sam Ratulangi University

References

ABDJUL, D.B., YAMAZAKI, H., KANNO, S., TOMIZAWA, A., WEWENGKANG, D.S., ROTINSULU, H., SUMILAT, D.A., UKAI, K., KAPOJOS, M.M. and NAMIKOSHI, M. (2017) An Anti-mycobacterial Bisfunctionalized Sphingolipid and New Bromopyrrol Alkaloid from the Indonesian Marine Sponge Agelas sp. Journal of Natural Medicines, 71 (3), pp. 531-536.

ABDJUL, D.B., YAMAZAKI, H., KANNO, S., TAKAHASHI, O., KIRIKOSHI, R., UKAI, K., and NAMIKOSHI, M. (2016) Haliclonadiamine derivatives and 6-epi-Monanchorin from the marine sponge Halichondria panicea collected at Iriomote island. Journal of Natural Products, 79 (4), pp. 1149-1154.

BU, Y.Y., YAMAZAKI, H., UKAI, K. and NAMIKOSHI, M. (2014) Anti-mycobacterial nucleoside antibiotics from a marine-derived Streptomyces sp. TPU1236A. Marine Drugs, 12 (12), pp. 6102-6112.

CARROLL, A.R., COPP, B.R., DAVIS, R.H., KEYZERS, R.A. and PRINSEP, M.R. (2020) Marine natural products. Natural Product Reports, 37, pp. 175-223.

CLSI (2013) Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement M100-S23; Clinical and Laboratory Standards Institute: Wayne, PA, USA. p. 205.

EBADA, S.S., EDRADA, R.U., LIN, W. and PROKSCH, P. (2008) Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrates. Nature Protocols, 3 (12), pp. 1820-1831.

MAARISIT, W., YAMAZAKI, H., ABDJUL, D.B., KATO, H., ROTINSULU, H., WEWENG-KANG, D.S., SUMILAT, D.A., KAPOJOS, M.M., UKAI, K. and NAMIKOSHI, M. (2017) Anti-Mycobacterial Alkaloids, Cyclic 3-Alkyl Pyridinium Dimers, from the Indonesian Marine Sponge Haliclona sp. Bioorganic & Medicinal Chemistry Letters, 27 (15), pp. 3503-3506.

MEHBUB, M.F., LEI, J., FRANCO, C. and ZHANG, W. (2014) Marine Sponge Derived Natural Products between 2001 and 2010: Trends and Opportunities for Discovery of Bioactives. Marine Drugs, 12, pp. 4539-4577.

NEWMAN, D.J. and CRAGG, G.M. (2014) Marine-sourced anti-cancer and cancer pain control agents in clinical and late preclinical development. Marine Drugs, 12 (1), pp. 255-278.

NEWMAN, D.J. and CRAGG, G.M. (2020) Natural Products as Sources of New Drugs over the

Nearly Four Decades from 01/1981 to 09/2019. Journal of Natural Products, 83 (3), pp. 770-803.

SUMILAT, D.A. (2019) Antibacterial Activity ODS Fractions of Marine Sponge Auletta sp. against Mycobacterium smegmatis. Jurnal Ilmiah Platax, 7 (1), pp. 329-334.

WHO (2020) Global Tuberculosis Report 2020. Geneva: World Health Organization. p. 232. Licence: CC BY-NC-SA 3.0 IGO. ISBN 978-92-4-001313-1.

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Published

2021-04-30

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