Isolasi dan Identifikasi Jamur Entomopatogen Isolat Tomohon dari Larva Ulat Grayak Spodoptera frugiperda (Lepidoptera: Noctuidae)
DOI:
https://doi.org/10.35799/jbcw.v1i1.35791Keywords:
Metarhizium rileyi, entomopathogen, Spodoptera frugiperdaAbstract
The armyworm frugiperda (Spodoptera frugiperda) is a dangerous invasive pest that attacks corn plantations in Tomohon City. In fact in the field there are entomopathogenic fungi that infect the armyworm frugiperda, but so far there is no data on entomopathogenic fungi isolated from this location. This study aimed to isolate and identify the entomopathogenic fungus Tomohon from the Larvae of S. frugiperda. Infected larvae were isolated and morphologically identified at the Laboratory of Biological Agents BPPMTPH Kalasey, and molecular identification at the Microbiology Laboratory, FMIPA Unsrat. The results showed that the entomopathogenic fungus that attacks the larvae of S. frugiperda is a species of Metarhizium rileyi which has macroscopic morphological characteristics with a rounded colony surface shape, and the color of the colony in the early phase of vegetative growth is white and when it enters the generative phase until the isolate changes color to dark green, conidia ovate, conidiophores straight and septate, branches grow near the septa, smooth walls and hyaline.
Abstrak
Ulat grayak frugiperda (Spodoptera frugiperda) merupakan salah satu hama invasif berbahaya yang menyerang perkebunan jagung di Kota Tomohon. Pada kenyataan di lapangan terdapat jamur entomopatogen yang menginfeksi ulat grayak frugiperda, namun sampai saat ini belum ada data jamur entomopatogen yang diisolasi dari lokasi ini. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi jamur entomopatogen isolat Tomohon dari larva S. frugiperda. Larva yang terinfeksi diisolasi dan diidentifikasi morfologi di Laboratorium Agens Hayati BPPMTPH Kalasey, dan identifikasi molekuler di Laboratorium Mikrobiologi FMIPA Unsrat. Hasil penelitian menunjukkan bahwa jamur entomopatogen yang menyerang larva S. frugiperda merupakan spesies Metarhizium rileyi memiliki ciri-ciri morfologi makroskopis dengan bentuk permukaan koloni yang membulat, dan warna koloni pada fase diawal pertumbuhan vegetatifnya berwarna putih dan saat akan memasuki fase generatif hingga isolat berubah warna menjadi hijau tua, bentuk konidia bulat telur, konidiofor lurus dan berseptat, cabang tumbuh didekat septat, berdinding halus dan hialin.
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