Effects of Clove Syrup Therapy on Creatinine, Urea, and Kidney Histology of Diabetes Mellitus Rat

Authors

  • Firly Sembiring
  • Adrien Jems Akiles Unitly
  • La Eddy

DOI:

https://doi.org/10.35799/jbcw.v1i1.36184

Keywords:

Clove Syrup, Rat, Diabetes Mellitus, Diabetes Nephropathy, Blood Glucose, Urea, Creatinine, Kidney Histology.

Abstract

This study aims to determine the effect of clove syrup on creatinine, urea, and kidney histology of diabetic rats induced by streptozotocin. Eighteen male rats were divided into six groups: N, C (-), and C (+) were the control groups, and groups 1.8, 3.6, and 7.2 were the experimental groups. Groups C (-), C (+), 1.8, 3.6, and 7.2 were injected with streptozotocin 45 mg/kg BW in a single intraperitoneal injection. Normal control group or N and negative control group or C (-) were left untreated; positive control group or C (+) were treated with glibenclamide 0.09 mg/kg BW; groups 1.8, 3.6, and 7.2 were given clove syrup with doses of 1.8, 3.6, and 7.2 ml/rat/day respectively for 14 days. The obtained data were analyzed using a one-way ANOVA test, followed by the Duncan test at the significant level of 95% (a = 0.05). Kidney histology was observed by Hematoxylin-Eosin staining using Microscope. The result showed that clove syrup therapy with a dose of 3.6 ml/rat/day significantly decreases glucose, urea, and creatinine levels. Also, it showed that the dose of 3.6 has a regeneration effect to repair the damaged kidney. However, the higher amount of 7,2 ml/rat/day was toxic, causing an increase in blood glucose, urea, and creatinine levels and impairing kidney cells. In conclusion, clove syrup consumption at the right dose gave the best result in decreasing glucose, urea, and creatinine levels and regenerating the damaged kidney.

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Published

2021-10-09