Lactococcus garvieae Isolates from Rainbow Trout (Oncorhynchus mykiss, W.) Compared by PLG and SA1B10 PCR Primer Pairs

Authors

  • Erbülent Altan AKDENIZ UNIVERSITY FACULTY OF FISHERIES
  • Jale Korun AKDENIZ UNIVERSITY FACULTY OF FISHERIES CAMPUS ARAPSUYU ANTALYA TURKEY

DOI:

https://doi.org/10.35800/jip.9.1.2021.32561

Keywords:

Lactococcus garvieae, Rainbow Trout, PCR, Antibiotic

Abstract

The aim of this study was to identify and compare Lactococcus garvieae strains using the PLG primer pair and SA1B10 primer pair. Also, the antibiotic resistance of the strains was investigated in the study. For this aim, commercial trout farms at Kemer, Korkuteli, and Manavgat, Antalya Province, Turkey were visited every month from June to September 2018. Thirty sick fish were sampled in the study. Lethargy, anorexia, darkening of skin color, unilateral or bilateral exophthalmos, opacification and hemorrhages in the eyes of the sick fish, and abdominal dropsy were observed. At necropsy, hemorrhages in the internal organs, splenomegaly, and darkening of the spleen and ascites were present. Seventy-five strains from sick fish samples verified on the basis of the biochemical characterization and PCR studies. The strains showed homogeneity in terms of phenotypic characteristics and all strains were identified as Lactococcus garvieae. The PCR technique was applied to 50 of 75 strains using PLG primer pair. While 47 of 50 strains gave positive results, amplification was not observed in 3 strains. When the PCR technique using SA1B10 primer pair was applied to a total of 25 strains, 3 of which were negative, 22 of which gave positive amplification, and 25 strains gave positive results. Resistance was determined by the disk diffusion method. All seventy-five strains were resistant to ampicillin and all strains were susceptible to bacitracin and tetracycline. It was found that the resistance and susceptibility of the strains showed variation to other antibiotics used in the study.

Keywords: Lactococcus garvieae; Rainbow Trout; PCR; Antibiotic

References

Abachi S., Lee S., Vasantha R., Singhhe H. P. 2016. Molecular mechanisms of inhibition of Streptococcus species by phytochemicals. Molecules, 21(215), 1-31.

Aguado Urdo M., López Campos G. H., Fernández Garayzábal J. F., Martin Sánchez F., Gibello A., Domínguez C., Blanco M. 2010. Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization. BMC Microbiology, 10(79), 1-8.

Altun S., Kubilay A., Ekici S., Didinen B. I., Diler Ö. 2010. Oral vaccination against lactococcosis in rainbow trout (Oncorhynchus mykiss) using sodium alginate and poly(lactide-co-glycolide) carrier. Kafkas Univ Vet Fak Derg. 16(B), 211-217.

Aoki T., Park C. I., Yamashita H., Hirono I. 2000. Species-specific polymerase chain reaction primers for Lactococcus garvieae. J Fish Dis. 23, 1-6.

Aron Y. 2017. Pond management practices, occurrence and antimicrobial resistance of Streptococcus and Lactococcus species in cultured tilapia in Morogoro, Tanzania. Ph. Thesis, University of Sokoine, Tanzania, 72 p.

Austin B., Austin D. A. 2012. Bacterial Fish Pathogens, Disease of Farmed and Wild Fish, Springer, U.K.

Baker J. S. 1984. Comparison of various methods for differentiation of Staphylococci and Micrococci . J Clin Microbiol. 19, 875-879.

Diler Ö., Altun S., Adiloğlu A. K., Kubilay A., Işıklı B., 2002. First occurrence of streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bull Eur Ass Fish Pathol. 22(1), 21-26.

Eraclio G., Ricci G., Quattrini M., Morani P., Fortina M. G. 2018. Detection of virulence-related genes in Lactococcus garvieae and their expression in response to different conditions. Folia Microbiol. 63(3), 291-298.

EUCAST, 2011. European Committee on Antimicrobial Susceptibility Testing, Breakpoint tables for interpretation of MICs and zone diameters.

Ferrario C., Ricci G., Milani C., Lugli G. A., Ventura M., Eraclio G., Borgo F., Fortina M. G. 2013. Lactococcus garvieae: Where is it from? A first approach to explore the evolutionary history of this emerging pathogen. PLos ONE, 8(12), e84796, doi: 10.1371/journal.pone.0084796.

Fukushima H. C. S., Leal C. A. G., Cavalcante R. B., Figueiredo H. C. P., Arijo S., Moriňigo M. A., Ishikawa M., Borra R. C., Ranzani Paiva M. J. T. 2017. Lactococcus garvieae outbreaks in Brazilian farms lactococcosis in Pseudoplatystoma sp.- development of an autogenous vaccine as a control strategy. J Fish Dis. 40, 263-272.

Gibello A., Galán Sánchez F., Mar Blanco M., Rodríguez Iglesias M., Domínguez L., Fernández Garayzábal J. F. 2016. The zoonotic potential of Lactococcus garvieae: an overview on microbiology, epidemiology, virulence factors and releationship with its presence in foods. Res Vet Sci. 109, 59-70.

Idowu T. A., Adedeji H. A., Sogbesan O. A. 2017. Fish disease and health management in aquaculture production. Int J and AgriSci. 1(1), 1-6.

Kav K., ErganiÅŸ O. 2008. Antibiotic susceptibility of Lactococcus garvieae in rainbow trout (Oncorhynchus mykiss) farms. Bull Vet Inst Pulawy. 52, 223-226.

Kia E. R., Mehrabi Y. 2013. Detection and identification of different streptococcosis strains in farmed rainbow trout in Boyerahmad and Dena regions (North South of Iran). WJFMS. 5(3), 315-321.

Kim W., Park H. K., Thanh H. D., Lee B., Shin J. W., Shin H. 2011. Comparative genome analysis of Lactococcus garvieae using a suppression subtractive hybridization library: discovery of novel DNA signatures. FEMS Microbiology Letters, 325, 77-84.

Kirkan S., Parin V., Dolgun O. 2018. Identification of Lactococcus garvieae by PCR from rainbow trout and investigation of susceptibility to antibiotics. IJVS, 7(1), 28-32.

Korun J., Timur G., Yardımcı R. E., Balcı B. A. 2018. A study on detecting infectious caused by some coccal species on the cultured rainbow trout (Oncorhynchus mykiss, W.). Atatürk Üniversitesi Vet Bil Derg. 13(2), 149-158.

Nakai T., Park S. C. 2002. Bacteriophage therapy of infectious disease in aquaculture. Res Microbiol. 153, 13-18.

Pereira F., Cavelo C., Toranzo A. E., Romalde J. L. 2004. Lactococcus garvieae, an emerging pathogen for the Portuguese trout culture. Bull Eur Ass Fish Pathol. 24(6), 274-279.

Savvidis G. K., Anatoliotis C., Kanaki C., Vafeas G. 2007. Epizootic outbreaks of lactococcosis disease in rainbow trout, Oncorhynchus mykiss (Walbaum), culture in Greece. Bull Eur Ass Fish Pathol. 27(6), 223-228.

Sharifi Yazdi H., Akhlaghi M., Tabatabaei M., Mostafavi Zadeh S. M. 2010. Isolation and characterization of Lactococcus garvieae from diseased rainbow trout (Oncorhynchus mykiss) cultured in Iran. J Vet Res. 11(4), 342-350.

Timur G., Yardımcı R. E., Ürkü Ç., Çanak Ö. 2011. Diagnosis of lactococcosis by bacteriological and histopathological methods in farmed rainbow trout (Oncorhynchus mykiss, L.) at the Marmara Region. İstanbul University, Journal of Fisheries and Aquatic Sciences, 26, 63-81.

Tsai M. A., Wang P. C., Yoshida T., Liaw L. L., Chen S. C. 2013. Development of a sensitive and specific LAMP PCR assay for detection of fish pathogen Lactococcus garvieae. Dis Aquat Org. 102, 225-235.

Vendrell D., Balcázar J. L., Ruiz Zarzuela I., Blas I., Gironés O., Múzquiz J. L. 2006. Lactococcus garvieae in fish: A review. Comp Immunol Microbiol Infect. 29, 177-198.

Zlotkin A., Eldar A., Ghittino C., Bercovier H. 1998. Identification of Lactococcus garvieae by PCR. J Clin Microbiol. 36(4), 983-985.

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Published

2021-02-23

How to Cite

Altan, E., & Korun, J. (2021). Lactococcus garvieae Isolates from Rainbow Trout (Oncorhynchus mykiss, W.) Compared by PLG and SA1B10 PCR Primer Pairs. Jurnal Ilmiah PLATAX, 9(1), 18–28. https://doi.org/10.35800/jip.9.1.2021.32561