Antibacterial Activity and Zoochemical Analysis of Sea Urchin Diadema setosum (Leske, 1778) Extract From Aertembaga Waters, Bitung City
DOI:
https://doi.org/10.35800/jip.v10i2.42322Keywords:
Sea urchin (D. setosum) gonad, antibacterial, disc diffusion, zoochemistry, extractionAbstract
Sea urchin is one of the marine biotas that produce bioactive compounds and has biological activity, one of which is antibacterial. This study aims to determine the antibacterial activity of the crude extract of D. setosum gonad and its fractionation against test bacteria Staphylococcus aureus and Escherichia coli and to conduct a zoochemical analysis to determine the content of bioactive compounds. The antibacterial test used the disc diffusion method while the chemical analysis was carried out qualitatively. The results showed that the methanol, ethyl acetate, and n-hexane fraction had antibacterial activity against the two test bacteria. The ethyl acetate fraction was the fraction that showed the highest antibacterial activity, its inhibition zone was 8 mm against S. aureus and 7,5 mm against E. coli. The zoochemical analyses of the ethyl acetate fraction from gonads extract showed positive results for alkaloid compound, phenolic, and saponin
Keywords: Sea urchin Gonad, Antibacterial, Disc diffusion, Zoochemistry, Extraction
Abstrak
Bulu babi merupakan salah satu biota laut yang memproduksi senyawa bioaktif dan memiliki aktivitas biologis salah satunya adalah antibakteri. Tujuan penelitian ini mengetahui aktivitas antibakteri dari ekstrak kasar gonad bulu babi D. setosum dan hasil fraksinasi terhadap bakteri Staphylococcus aureus dan Escherichia coli serta melakukan analisis zookimia untuk mengetahui kandungan senyawa bioaktif. Pengujian antibakteri menggunakan metode difusi cakram sedangkan analisis kimia dilakukan secara kualitatif. Hasil penelitian menunjukkan baik fraksi metanol, fraksi etil asetat, dan fraksi n-heksana memiliki aktivitas antibakteri terhadap kedua bakteri uji. Fraksi etil asetat merupakan fraksi yang memperoleh aktivitas antibakteri tebaik dengan diameter zona hambat 8 mm terhadap bakteri S.aureus dan 7,5 mm terhadap bakteri E. coli. Hasil uji zookimia fraksi etil asetat menunjukkan bahwa ekstrak gonad D. setosum mengandung senyawa dari golongan alkaloid, fenolik, dan saponin.
Kata kunci : Gonad Bulu Babi (D. setosum ), Antibakteri, difusi cakram, Zookimia, Ekstraksi
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