UJI TOKSISITAS AKUT INFUSA DAUN SIRSAK (Annona muricata) PADA LARVA Artemia salina MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

Arinta Mayang, Bilal S A Santoso

Abstract


ABSTRACT
Annona muricata is a family of Annonaceae that has been known as a medicinal plant. Sirsak
leaves contain alkaloids, tannins, and several other chemical contents including acetogenin.
The aim of this study was to determine the acute toxicity of the infusion of Sirsak leaves on
the Artemia salina larvae by the Brine Shirmp Lethality Test (BSLT) method. This experimental
study used 5 treatment concentrations (10 mg/L, 50 mg/L, 100 mg/L, 500 mg/L, 1000
mg/L) and 1 negative control. All treatments were repeated 3 times. The number of Artemia
salina larvae used for each concentration was 10 larvae. The number of dead larvae was
counted after 24 hours of treatment. Based on probit analysis, the LC50 value of Sirsak leaf
infusion was 38,73 mg/L. The conclusion of this research is the infusion of Sirsak leaves is
toxic.
Keywords: Sirsak leaves, toxicity, BSLT
ABSTRAK
Sirsak (Annona muricata) adalah keluarga Annonaceae yang telah dikenal sebagai tanaman
obat. Daun Sirsak mengandung alkaloid, tanin, dan beberapa kandungan kimia lainnya termasuk
acetogenin. Tujuan dari penelitian ini adalah untuk menentukan toksisitas akut dari infus
daun Sirsak pada larva Artemia salina dengan metode Brine Shirmp Lethality Test (BSLT).
Studi eksperimental ini menggunakan 5 konsentrasi perlakuan (10 mg/L, 50 mg/L, 100 mg/L,
500 mg/L, 1000 mg/L) dan 1 kontrol negatif. Semua perawatan diulang 3 kali. Jumlah larva
Artemia salina yang digunakan untuk setiap konsentrasi adalah 10 larva. Jumlah larva mati
dihitung setelah 24 jam perlakuan. Berdasarkan analisis probit, nilai LC50 infus daun Sirsak
adalah 38,73 mg/L. Kesimpulan penelitian ini adalah infus daun Sirsak bersifat toksik.
Kata kunci: daun sirsak, toksisitas, BSLT


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DOI: https://doi.org/10.35799/pmj.3.1.2020.28960

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