Potensi Bakteri Endofit Ubi Jalar (Ipomoea batatas L.) dalam Menghasilkan Hormon Indole Acetic Acid (IAA) dengan Penambahan L-triptofan
Abstract
Potensi Bakteri Endofit Ubi Jalar (Ipomoea batatas L.) dalam Menghasilkan Hormon Indole Acetic Acid (IAA) dengan Penambahan L-triptofan
(Potential of endophytic bacteria of sweet potato (Ipomoea batatas L.) in producing Indole Acetic Acid (IAA) with the addition of L-tryptophan)
Agustina Monalisa Tangapo
Program Studi Biologi FMIPA Universitas Sam Ratulangi
Jl. Kampus Unsrat, Manado 95115
*Email korespondensi:agustina.tangapo@unsrat.ac.id
(Article History: Received 5-01-2019; Revised 15-01-2020; Accepted 05-02-2020)
ABSTRAK
Asosiasi bakteri-tanaman, dapat mempengaruhi produktivitas tanaman secara langsung dan tidak langsung. Secara langsung, salah satunya yaitu bakteri dapat memproduksi dan menyekresikan zat pengatur tumbuh indole-3-acetic acid (IAA, auksin). Penelitian ini bertujuan untuk menguji kemampuan bakteri endofit ubi jalar dalam menghasilkan IAA. Metode analisis IAA dilakukan dengan metode kolorimetri. Analisis produksi IAA dilakukan dengan penambahan dan tanpa penambahan L-triptofan. Hasil penelitian menunjukkan bahwa tanpa penambahan L-triptofan, diperoleh sejumlah 19 jenis yang menghasilkan IAA dengan kisaran konsentrasi 0,29-7,21 mg/L. Dengan penambahan L-triptofan, jumlah jenis positif dan konsentrasi IAA yang dihasilkan meningkat signifikan. Jumlah jenis positif 20 jenis (91%) dan konsentrasi IAA yang dihasilkan mencapai kisaran 0,96-115,63 mg/L.
Kata kunci: bakteri endofit; IAA; ubi jalar; L-triptofan
ABSTRACT
Plant-bacteria associations, can promote plant growth by both direct and indirect mechanisms. One of direct mechanisms is that bacteria can produce and secrete indole-3-acetic acid (IAA, auxin) growth regulators. This study aims to examine the ability of sweet potato endophytic bacteria to produce IAA. The detection of IAA production was conducted by colorimetric technique. IAA production analysis was carried out with addition and without addition of L-tryptophan. Without the addition of L-tryptophan, a total of 19 species produced IAA with a concentration range of 0.29-7.21 mg/L. With the addition of L-tryptophan, the number of positive species and the concentration of IAA produced increased significantly. The number of positive species was 20 species (91%) and the concentration of IAA produced reached a range of 0.96-115.63 mg/L.
Keywords: endophytes bacterial; IAA; sweet potato; L-tryptophanFull Text:
PDFDOI: https://doi.org/10.35799/jbl.10.1.2020.27980
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Faculty of Mathematics and Natural Sciences