Aktivitas Enzim Ekstraseluler dari Bakteri Endofit Tumbuhan Mangrove Avicennia marina

Chindy Achika Rori, Febby Ester Fany Kandou, Agustina Monalisa Tangapo

Abstract


ABSTRAK

Bakteri endofit merupakan salah satu sumber penghasil senyawa ekstraseluler yaitu enzim. Enzim dari bakteri endofit lebih menguntungkan dan produksinya lebih cepat. Penelitian ini bertujuan untuk menganalisis kemampuan aktivitas enzim ekstraseluler dari bakteri endofit tumbuhan mangrove Avicennia marina. Metode penelitian yang digunakan yaitu eksploratif eksperimental, melakukan isolasi bakteri dari tumbuhan mangrove A. marina dan selanjutnya dilakukan uji potensi aktivitas enzim ekstraseluler dari isolat bakteri endofit. Hasil isolasi memperoleh tujuh isolat bakteri endofit dari tumbuhan A. marina, isolat endofit tersebut mampu menghasilkan aktivitas enzim ekstraseluler yaitu empat isolat menghasilkan enzim amilase, enam isolat menghasilkan protease, satu isolat menghasilkan selulase dan dua isolat menghasilkan gelatinase.

Kata kunci: bakteri endofit; Avicennia marina; amilase; protease; selulase; gelatinase

 

ABSTRACT

Endophytic bacteria are one source that can produce extracellular compounds, namely enzymes. Enzymes from endophytic bacteria are more profitable and can produce faster. This study aims to analyze the ability of extracellular enzyme activity from endophytic bacteria in mangrove plants Avicennia marina. This research used experimental explorative method, isolating bacteria from mangrove plant A. marina and then testing the potential of enzyme extracellular activity from endophytic bacteria isolated. Isolating result obtained seven endophytic bacterial isolates from A. marina plants,this endophytic isolates are able to produce extracellular enzyme activity is four isolates can produced amylase enzyme, six isolated can produced protease, one isolated can produced cellulase and two isolated can produce gelatinase.

Keywords: Endophytic bacteria; Avicennia marina; amylase, protease, cellulase, gelatinase

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DOI: https://doi.org/10.35799/jbl.11.2.2020.28338

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Copyright (c) 2020 Chindy Achika Rori, Febby Ester Fany Kandou, Agustina Monalisa Tangapo

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Publisher:
Department of Biology
Faculty of Mathematics and Natural Sciences
Universitas Sam Ratulangi. Manado, North Sulawesi


 

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