Pengaruh Lama Perendaman Basis Gigi Tiruan Resin Asetal dalam Larutan Hidrogen Peroksida 3% sebagai Denture Cleanser terhadap Perlekatan Candida albicans

Abstract

Abstract: Denture cleanser could control biofilm formation, however, it could also cause noticable changes to the surface denture base material, such as an increase in surface roughness and discoloration. This study aimed to evaluate the effect of immersion time of acetal resin denture base in H₂O₂ 3% as denture cleanser on the attachment of Candida albicans. Samples were made of acetal resin in the form of disc with a diameter of 5 mm and height of 2 mm as many as 24 pieces which were divided into four groups as follows: six discs without treatment, six discs were immersed in H₂O₂ 3% for 3.5 hours, six discs were immersed for 14 hours, and six discs were immersed for 42 hours. The number of Candida albicans colonies was calculated in units of CFU/ml. The results  showed that there was an effect of H₂O₂ 3% immersion time on the adhesion of Candida albicans in all immersion groups after being compared with the control group. The highest number of Candida albicans attachment was found on the group with 42 hours immersion time. In conclusion, the immersion time of acetal resin denture base in H₂O₂  3% for 3,5 hours had the lowest effect on the adhesion of Candida albicans compared to the duration of immersion for 14 and 42 hours.

Keywords: acetal resin; denture cleanser; hydrogen peroxide 3%; Candida albicans

Abstrak: Denture cleanser dapat mengontrol pembentukan biofilm, tetapi juga mengakibatkan perubahan yang terlihat pada permukaan basis gigi tiruan seperti peningkatan kekasaran permukaan dan perubahan warna. Penelitian ini bertujuan untuk mengevaluasi pengaruh lama perendaman basis gigi tiruan resin asetal dalam H₂O₂ 3% sebagai denture cleanser terhadap perlekatan Candida albicans. Sampel dibuat dari cakram resin asetal dengan diameter 5 mm dan tinggi 2 mm sebanyak 24 buah yang dibagi menjadi empat kelompok yaitu, 6 cakram tanpa perlakuan, 6 cakram direndam dalam H₂O₂ 3% selama 3,5 jam, 6 cakram direndam selama 14 jam, dan 6 cakram direndam selama 42 jam. Jumlah Candida albicans dihitung dalam satuan CFU/ml unit. Hasil penelitian memperlihatkan bahwa terdapat pengaruh lama perendaman dalam H₂O₂ 3%terhadap perlekatan Candida albicans pada semua kelompok setelah dibandingkan dengan kelompok kontrol. Jumlah Candida albicans tertinggi ditemukan pada kelompok dengan lama perendaman selama 42 jam. Simpulan penelitian ini ialah lama perendaman basis gigi tiruan resin asetal dalam H₂O₂ 3% selama 3,5 jam memiliki pengaruh paling rendah terhadap perlekatan Candida albicans dibandingkan dengan lama perendaman 14 jam dan 42 jam.

Kata kunci: resin asetal; denture cleanser; hidrogen peroksida 3%; Candida albicans