IDENTIFIKASI VARIETAS KENTANG “SUPERJOHN” BERDASARKAN PENANDA RAPD (Random Amplified Polymorphic DNA)

Abstract

ABSTRACT

Identification using morphological characters has time consuming. Currently, identification using molecular markers has now been popular due to rapid, saving time and more precisely. Superjohn potato variety has been cultivated in North Sulawesi. However, the Superjohn potato variety has not been characterized using molecular markers. This research was aiming to identify “Superjohn” potato based on RAPD (Random Amplified Polymorphic DNA) markers. The research was conducted in field and laboratory. Field research was performed by taking some young leaves from “Superjohn”, Granola, and Atlantic variety from the field. Identification using molecular marker was conducted at laboratory.  Nine RAPD primers were used to identify the superjohn variety. The nine primers were OPA-1, OPA-2, OPA-3, OPA-4, OPA-5, OPA-7, OPA-9,  OPA-10, and  OPO-1.  The molecular identification revealed that “Superjohn” variety was different with Granola and Atlantic. OPA-9₇₀₀ primer could be used for identification  “Superjohn” variety while OPA-10₁₀₀₀ primer was suitable  for identification  Granola variety.

Keywords:  Potatoes, variety, “Superjohn”, Granola, Atlantic, and RAPD

ABSTRAK

Penelitian ini dilakukan untuk mengidentikasi kentang “Superjohn” berdasarkan penanda RAPD (Random Amplified Polymorphic DNA).  Penelitian dilakukan di lapangan dan laboratorium. Penelitian lapangan dilakukan dengan mengambil beberapa daun muda dari varietas kentang “Superjohn”, Atlantik dan Granola. Kemudian  analisis DNA dilakukan di laboratorium menggunakan analisis RAPD. Berdasarkan penanda RAPD ternyata kentang “Superjohn” berbeda dari kentang Granola dan kentang Atlantik. Penanda RAPD OPA-9₇₀₀ dapat digunakan untuk mengidentifikasi kentang“Superjohn” dan penanda RAPD  OPA-10₁₀₀₀ dapat digunakan  untuk mengidentifikasi kentang Granola.

Kata kunci:  Kentang,  varietas, Superjohn, Granola, Granola, RAPD