PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

Alfrida ., Maninggolang, Jeany Sh. Polii-Mandang, Wenny ., Tilaar

Abstract


This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.


Keywords


Brassica oleraceae var italic plenck, Benzyl Amino Purine (BAP), Coconut Water, Sulforaphane

Full Text:

PDF PDF

References


Anonim,. (1993). Peran Air Kelapadalam Kultur Jaringan Tanaman Krisan. Disertasi. Istitut Pertanian Bogor.

Anonim,. (1995). Air Kelapa Sebagai Bahan Subtitusi Media MS pada Kultur Jaringan Krisan. Eugenia 1(1) : 1-11.

Apriadji, W. H. (2008). Menimbang Keunggulan Sayuran Daun. Retrieved Maret 7, 2017, from multiply: http://kulinerkita.muliply.

com/reviews/items/506.

Bey, Y., Syafii, W., & Sutrisna. (2006). Pengaruh Pemberian Giberalin (GA3) dan air kelapa terhadap perkecambahan biji anggrek bulan (Phalaenopsis amabilis BL) secara in vitro. j. Biogenesis 2 (2), 41-46.

Chowdhury, A. R., & Chaturvedi, H. C. (1980). Cholesterol and Bioshynthesis of diosgenin by tuber callus of Dioscore deltoidea. Curren Science, (49) : 237-8.

Fithriyandini, A., Maghfoer, D. M., & Wardiyati, T. (2015). Pengaruh Media Dasar dan 6-Benzylaminopurine (BAP) terhadap Pertumbuhan dan Perkembangan Nodus Tangkai Bunga

Anggerk Bulan (Phalaenopsis amabilis) dalam perbanyakan secara

in vitro.

George, E. F., & Sherrington, P. D. (1984). Plant Propagation by Tissue Culture. England: Exegetics Limited.

Isda, N. M., Fatonah. S. (2014). Induksi Akar Pada Eksplan Tunas Anggrek Gramatophylum scriptum var. Citrinum Secara in vitro pada Media MS dengan Penambahan NAA dan BAP.

Klerk, G. J. (2006). Plant Hormones In Tissue Culture, In Duchefa Biochemie. Biochemicals Plant Cell And Tissue Culture Phytopathogy, Duchefa Biochemie BV, Haarlem. Netherlands.

Kumar, M. S Balachandran, S., Chaudhury, S. (2012). Influence of incubation temperatures on total phenolic, flavonoids content an free radical scavenging activity of callus from Heloitropium indicum L. Asia J. Pharm. Res, 2 (4): 148-152.

Mandang, J. S. (2013). Media Kultur Jaringan Tanaman. Manado: Bayumedia Publishing Anggota IKAPI.

Mantell, S. H., Matthews, J. A and R. A. MC. Kee. (1983). Principles of Plant Biotechnology. An Introduction to Genetic Engineering in Plant. Blackwell Scientific Publ. London. Melbourne. P. 197-204.

Matatula, A. J. (3003). Subtitusi Media MS dengan Air Kelapa dan Gandasil-D Pada Kultur Jaringan Krisan. Eugenia

(4) : 203-211.




DOI: https://doi.org/10.35791/agrsosek.14.1.2018.19730

Refbacks

  • There are currently no refbacks.