TOKSISITAS EKSTRAK FUNGI YANG BERASOSIASI DENGAN SPONGE Acanthostrongylophora ingens ASAL PERAIRAN PULAU BANTONG KABUPATEN BOLAANG MONGONDOW TIMUR TERHADAP LARVA NYAMUK Aedes aegypti

Abstract

The aim of this study is to isolate fungal symbionts from the sponge Acanthostrongylophora ingens that grow in Bantong Island Waters to test their larvicidal activity towards A. aegypti larvae. A piece of sponge A. ingens were cut and then planted on PDA media until the fungal mycelium starts to grow. The pure isolates are inoculated into a rise medium in the Erlenmeyer flasks for static incubation in room temperature for 14 days.  Furthermore, the isolates were soaked with ethyl acetate 3 times, followed by evaporation using a Rotary Vacuum evaporator. The extracts of each fungal isolates were tested on A. aegypti larvae. The results showed that the five fungal isolate extracts have anti-larval activity with LC₅₀ values in isolates ranging from 1 to 6 ppm, namely, 1.2 (5.094 ppm), isolates 1.3 (3.388 ppm), isolates 2.2 (1.614 ppm), isolates 2.1B (5.918 ppm), isolates 1.1.2 (6.220 ppm). From the LC50 results of this study, the extracts of the five isolates of the associated mushroom with the sponge A. ingens from the waters of Bantong Island, East Bolaang Mongondow Regency were categorized as very toxic according to the Tanamayat and Clarkson categories, so that they could be developed as anti-larval towards A. aegypti mosquito.

Keywords: Toxicity, Larvicidal, sponge Acanthostrongylophora ingens, fungal symbionts