Shell Color Classification And Carotenoid Pigments On Littoraria pallescens (Philippi, 1846) From Mangrove Ecosystem Area On Mokupa Village, Tombariri Sub District and Basaan Village, Ratatotok Sub District)

Susan M. Sumampouw, Desy M. H. Mantiri, Farnis B. Boneka, Medy Ompi, James J. H. Paulus, Adnan S. Wantasen

Abstract


The purpose of this study was to classify the color of the shell and to know the carotenoid pigment content in Littoraria pallescens based on color classification and population distribution in the mangrove ecosystem area of Mokupa Village, Tombariri Sub district and Basaan Village, Ratatotok Sub district. Sampling directly on mangrove trees as water begins to recede. Identification of L. pallescens species is done by looking at the shape of the shell, the color of the shell, the color of the operculum and the shape of the genital organ. Shell color classification by inserting into the Color Explorer application. Analysis of carotenoid pigments by extraction process with acetone and petroleum ether, further separation of pigment by thin layer chromatography. The results obtained show that L. pallescence dominates life on mangrove trees. Sex was inversely proportional to the two research sites, 53.8% of the male L. pallescens species and 46.2% female in Mokupa waters while from Basaan waters there were 47.1% males and 52.9% females. Color classification based on the percentage of occurrences of constant color that is black (18.5), black orange (16.3) brown black spots (16,3), gray (10.7), angry (6,3), yellow pale (17.8), brown yellow spots (14,1). The detected pigment based on the color classification of the shell is located on the identical and identifiable Rf for all colors is the β-carotene pigment.

Keyword : Littoraria pallescence, Carotenoid pigments

 

Tujuan dari penelitian ini adalah untuk mengklasifikasikan warna cangkang dan mengetahui kandungan pigmen karotenoid pada Littoraria pallescens berdasarkan klasifikasi warna dan sebaran populasinya di wilayah ekosistem mangrove Desa Mokupa kecamatan Tombariri dan Desa Basaan Kecamatan Ratatotok. Pengambilan sampel secara langsung pada pohon mangrove saat air mulai surut. Identifikasi spesies L. pallescens dilakukan dengan melihat bentuk cangkang, warna cangkang, warna operculum dan bentuk organ genital.  Pendataan untuk klasifikasi warna cangkang dengan memasukkan ke dalam aplikasi Color Explorer. Analisis pigmen karotenoid melalui proses ekstraksi dengan aseton dan petroleum eter, selanjutnya pemisahan awal pigmen dengan kromatografi lapis tipis. Hasil yang diperoleh menunjukan bahwa L. pallescence mendominasi hidup pada pohon mangrove. Jenis kelamin berbanding terbalik pada dua lokasi penelitian, spesies L. pallescens jantan 53.8% dan betina 46.2% di perairan Mokupa sedangkan dari perairan Basaan terdapat 47.1% jantan dan 52.9% betina. Klasifikasi warna berdasarkan persentase kemunculan warna yang konstan yaitu warna hitam (18,5), hitam oranye (16,3) coklat bercak hitam (16,3), abu-abu (10,7), Marah (6,3), kuning pucat (17,8), kuning bercak coklat (14,1). Pigmen yang terdeteksi berdasarkan klasifikasi warna pada cangkang adalah berada pada Rf yang sama dan yang dapat diidentifikasi untuk semua warna adalah pigmen ß-karoten.

Kata kunci : Littoraria pallescence, Pigmen Karotenoid

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DOI: https://doi.org/10.35800/jip.6.2.2018.20650

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Copyright (c) 2018 Susan M. Sumampouw, Desy M. H. Mantiri, Farnis B. Boneka, Medy Ompi, James J. H. Paulus, Adnan S. Wantasen

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

ISSN: 2302-3589

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Platax Scientific Journal under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

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