TOKSISITAS AKUT KOMBUCHA DAUN TIN (Ficus carica) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

Abstract

ABSTRACT
Fig leaves (Ficus carica) contain secondary metabolites such as flavonoids, tannins, alkaloids,
saponins and triterpenoids, which potentially toxic in a certain amount. Fermentation of fig
leaves using kombucha produce healthy drink with various bioactivities. The aim of this research
was to observe acute toxicity of fig leaves kombucha using Brine Shrimp Lethality
Test (BSLT) method. Toxixity test againts Artemia salina Leach larvae using 7 variations in
fig leaves kombucha concentration from 100 ppm to 30000 ppm. The results showed that fig
leaves kombucha LC50 value of 139,99 ppm, so that fig leaves kombucha is potentially toxic.
Key words: acute toxicity, BSLT, fermentation, fig leaves
ABSTRAK
Daun tin (Ficus carica) mengandung metabolit sekunder antara lain flavonoid, tanin, alkaloid,
saponin dan triterpenoid yang berpotensi toksik dalam jumlah tertentu. Fermentasi daun
tin oleh kultur kombucha menghasilkan minuman kesehatan dengan berbagai bioaktivitas.
Penelitian ini bertujuan untuk mengetahui toksisitas akut kombucha daun tin menggunakan
metode Brine Shrimp Lethality Test (BSLT). Pengujian toksisitas terhadap larva Artemia salina
Leach menggunakan 7 variasi konsentrasi kombucha daun tin antara 100 ppm sampai
30000 ppm. Hasil penelitian menunjukkan nilai LC50 kombucha daun tin sebesar 139,99
ppm, sehingga disimpulkan bahwa kombucha daun tin berpotensi toksik.
Kata kunci: BSLT, daun tin, fermentasi, toksisitas akut