Uji Sitotoksik Ekstrak Daun Murbei (Morul Alba L) Terhadap Sel Kanker Mcf-7 Dan Selektifitasnya
Abstract
Mulberry, Morus alba L., is traditionally used to treat various diseases such as bacterial, fungal, and protozoal. In addition, the society in South Sulawesi uses mulberry leaves as an anti-cancer. The present study aimed to investigate the cytotoxic effect of mulberry leaf extract on breast cancer cells MCF-7 and their selectivity against Vero normal cells. The dried mulberry leaves were extracted by maceration using 96% ethanol. The cytotoxic activity was determined using MTT method, while the selectivity index (SI) was calculated by comparing IC50 of MCF-7 cells against Vero cells. The results showed that the ethanol extract of mulberry leaves was toxic to MCF-7 cells with an IC50 of 75.32±2.78 µg/mL and also toxic to Vero cells (71.73±4.39 µg/mL). However, ethanol extract of mulberry leaves was not selective on MCF-7 cells with an SI value of 0.95. Our study indicates that mulberry could be considered an effective agent in the prevention of cancer, but further studies are necessary needed for safety assessment.
Keywords
Full Text:
PDFReferences
Abu-Darwish MS, Efferth T. 2018. Medicinal plants from near east for cancer therapy. Front Pharmacol 9; e56
Andreani NA, Renzi S, Piovani G, Ajmone Marsan P, Bomba L, Villa R, Ferrari M, Dotti S. 2017. Potential neoplastic evolution of Vero cells: In vivo and in vitro characterization. Cytotechnology 69(5); 741-50
Benov L. 2019. Effect of growth media on the MTT colorimetric assay in bacteria. PLoS One 14(8); e0219713
Carneiro BA, El-Deiry WS. 2020. Targeting apoptosis in cancer therapy. Nat Rev Clin Oncol 17(7); 395-417
Comsa S, Cîmpean AM, Raica M. 2015. The Story of MCF-7 breast cancer cell line: 40 years of experience in research. Anticancer Res 35(6); 3147-54
Esfahani K, Roudaia L, Buhlaiga N, Del Rincon SV, Papneja N, Miller WH, Jr. 2020. A review of cancer immunotherapy: From the past, to the present, to the future. Curr Oncol 27(Suppl 2); S87-S97
Fallah S, Hajihassan Z, Sadeghi A. 2017. Cytotoxicity effects of flavonoid extract of Morus alba leaves in Hela cell line. Asian J Biol Sci 10; 72-79
KEMENKES RI. 2018. Hasil utama RISKEDAS 2018. Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan RI. Jakarta
Kuruppu AI, Paranagama P, Goonasekara CL. 2019. Medicinal plants commonly used against cancer in traditional medicine formulae in Sri Lanka. Saudi Pharm J 27(4); 565-73
Manggau MA, Lukman M. 2012. Efek farmakologi tanaman antikanker yang digunakan oleh masyarakat suku Makassar Sulawesi Selatan. LepHas. Makassar
Mirabelli P, Coppola L, Salvatore M. 2019. Cancer cell lines are useful model systems for medical research. Cancers 11(8); e1098
Nam SY, Yi HK, Lee JC, Kim JC, Song CH, Park JW, Lee DY, Kim JS, Hwang PH. 2002. Cortex mori extract induces cancer cell apoptosis through inhibition of microtubule assembly. Arch Pharm Res 25(2); 191-96
Nordin ML, Abdul Kadir A, Zakaria ZA, Abdullah R, Abdullah MNH. 2018. In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231. BMC Complement Altern Med 18(1); e87
Pakki E, Alam G, Usmar, Syukur R, Muslimin L. 2019. Anticancer activity of selected medicinal plants indigenous to Duri ethnic. Int J Pharm Sci Res 11(Supplementary 1); 602-08
Pakki E, Tayeb R, Usmar U, Ridwan IA, Muslimin L. 2020. Effect of orally administered combination of Caulerpa racemosa and Eleutherine americana (Aubl) Merr extracts on phagocytic activity of macrophage. Res Pharm Sci 15(4); 401-09
Rai Y, Pathak R, Kumari N, Sah DK, Pandey S, Kalra N, Soni R, Dwarakanath BS, Bhatt AN. 2018. Mitochondrial biogenesis and metabolic hyperactivation limits the application of MTT assay in the estimation of radiation induced growth inhibition. Sci Rep 8(1); e1531
Schirrmacher V. 2019. From chemotherapy to biological therapy: A review of novel concepts to reduce the side effects of systemic cancer treatment (Review). Int J Oncol 54(2); 407-19
Siegel RL, Miller KD, Fuchs HE, Jemal A. 2021. Cancer Statistics, 2021. CA Cancer J Clin 71(1); 7-33
Tanumihardja M, Hastuti S, Nugroho JJ, Trilaksana AC, Natsir N, Rovani CA, Muslimin L. 2020. Viabilities of odontoblast cells following addition of haruan fish in calcium hydroxide. Open Access Maced J Med Sci 8(D); 58-63
Wardihan, Rusdi M, Alam G, Muslimin L, Manggau MA. 2013. Selective cytotoxicity evaluation in anticancer drug screening of Boehmeria virgata (Forst) Guill leaves to several human cell lines: HeLa, WiDr, T47D and Vero. Dhaka Univ J Pharm Sci 12(2); 123-26
DOI: https://doi.org/10.35799/pmj.v4i2.37665
Refbacks
- There are currently no refbacks.
Copyright (c) 2022 Lukman lukman, Marwati Marwati, Suwahyuni Mus, Reny Syahruni
![Creative Commons License](https://i.creativecommons.org/l/by-nc/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.