Jurnal Ilmiah Sains https://ejournal.unsrat.ac.id/v3/index.php/JIS <p><strong>Jurnal Ilmiah Sains</strong> (Journal of Scientific Sciences) is the Journals Published by Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, Indonesia. Jurnal Ilmiah Sains Published Twice a Year, i.e. April and October.</p> <p><strong>Jurnal Ilmiah Sains</strong> welcomes full research articles in the area of Mathematics and Natural Sciences from the following subject areas:</p> <ul> <li>Chemistry</li> <li>Biology</li> <li>Mathematics</li> <li>Statistics</li> <li>Computer Science</li> <li>Physics</li> </ul> <p><strong>Jurnal Ilmiah Sains has been indexed by main indexing: <a href="https://app.dimensions.ai/discover/publication?search_mode=content&amp;search_text=jurnal%20ilmiah%20sains&amp;search_type=kws&amp;search_field=full_search&amp;and_facet_source_title=jour.1366596" target="_blank" rel="noopener">Dimensions</a>; <a href="https://scholar.google.co.id/citations?hl=en&amp;user=GG0SdqkAAAAJ">Google Scholar</a>; <a href="https://sinta.kemdikbud.go.id/journals/profile/4779" target="_blank" rel="noopener">Sinta</a>; <a href="https://garuda.kemdikbud.go.id/journal/view/1043">GARUDA (Garda Rujukan Digital)</a>; <a href="http://olddrji.lbp.world/Search.aspx?J=jurnal%20ilmiah%20sains&amp;S=0">Directory of Research Journals Indexing (DRJI)</a>; <a href="https://journals.indexcopernicus.com/search/form?search=Jurnal%20Ilmiah%20Sains">Index Copernicus International (ICI)</a>; <a href="https://www.worldcat.org/title/jurnal-ilmiah-sains/oclc/1163973263&amp;referer=brief_results">WorldCat</a>; <a href="https://www.crossref.org/titleList/">Crossref</a>; <a href="https://onesearch.id/Search/Results?lookfor=jurnal+ilmiah+sains&amp;type=AllFields&amp;filter%5B%5D=collection%3A%22JURNAL+ILMIAH+SAINS%22&amp;filter%5B%5D=authorStr%3A%22Pasau%2C+Guntur%22">OneSearch</a>; <a href="https://www.base-search.net/Search/Results?type=all&amp;lookfor=djoni+hatidja&amp;ling=1&amp;oaboost=1&amp;name=&amp;thes=&amp;refid=dcresen&amp;newsearch=1">Base</a><a href="https://www.crossref.org/titleList/"><br /></a></strong></p> <p><strong>JURNAL ILMIAH SAINS</strong> is Accredited Third Grade (Sinta 3/S3), <a href="https://drive.google.com/uc?export=download&amp;id=1yJ3dEbTZVwHR1ZpDecM-ngBvDqMfHJMa">(Sertificate clik here)</a> for Five Years (2021-2026) based on Decree No: 105/E/KPT/2022 (April 7, 2022) by Ministry of Education, Culture, Research and Technology (KEMDIKBUDRISTEK), The Republic of Indonesia.</p> en-US <p>LICENCE: <a href="https://creativecommons.org/licenses/by-nc/4.0/" target="_blank" rel="noopener">CC-BY-NC</a></p> <p><a href="https://creativecommons.org/licenses/by-nc/4.0/" target="_blank" rel="noopener">This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License</a></p> jurnalsains@unsrat.ac.id (Djoni Hatidja) jurnalsains@unsrat.ac.id (Jurnal Ilmiah Sains) Sat, 08 Feb 2025 00:00:00 +0800 OJS 3.3.0.12 http://blogs.law.harvard.edu/tech/rss 60 Antioxidant Activity of Ethanol Extract of Breadfruit Peel (Artocarpus altilis (Park.) Fosberg) and Determination of Total Phenolic and Flavonoid Levels https://ejournal.unsrat.ac.id/v3/index.php/JIS/article/view/57255 <p>Breadfruit peel (<em>Artocarpus altilis</em> (Park.) Fosberg) contains phenolic compounds and flavonoids. These compounds have benefits as antioxidants. This study aims to determine the antioxidant activity of ethanol extract of breadfruit peel by using the ABTS (<em>2,2- Azinobis (3- Ethylbenzothaizoline-6- Sulfonic Acid) </em> method and determining the total phenolic and flavonoid levels. The skin of breadfruit is extracted using a method with a 96% ethanol solvent. The results of the extract were tested for antioxidant activity by ABTS and trolox methods as a comparison, and the total phenolic and flavonoid levels were determined by comparing gallic acid and quercetin using UV-VIS spectrophotometry. The results of the absorbance data were analyzed by linear regression to obtain IC₅₀ values. Total phenolic and flavonoid levels were expressed by mgGAE/gram extract and mgQE/gram extract. The results showed that the extract ethanol of breadfruit peel extracted by maceration method obtained IC₅₀ 47.86±0.07 ppm, trolox IC₅₀ 20.17±0.06 ppm. The determination of the total phenolic and flavonoid levels was obtained as 280.70±2.54 mg GAE/gram and 5,51±0.02 mgQE/gram extract. Antioxidant activity of ethanol extract of breadfruit peel is categorized as a very powerful antioxidant.</p> <p><strong>Keywords</strong>: antioxidants; breadfruit peel; flavonoids; phenolics</p> Febriana Wahyuningtyas, Ibrahim Arifin, Khoirul Anwar Copyright (c) 2025 Febriana Wahyuningtyas, Ibrahim Arifin, Khoirul Anwar https://creativecommons.org/licenses/by-nc/4.0 https://ejournal.unsrat.ac.id/v3/index.php/JIS/article/view/57255 Sat, 08 Feb 2025 00:00:00 +0800 Antioxidant, Antibacterial, Cytotoxic, and Anticancer Potential of Belulang Grass (Eleusine indica) https://ejournal.unsrat.ac.id/v3/index.php/JIS/article/view/58431 <p>A study was conducted to determine the antioxidant, antibacterial, cytotoxic, and anticancer activities of the belulang grass plant (<em>Eleusine indica</em>). Antioxidant activity was assessed using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity method. Antibacterial activity was determined using the disk diffusion method, cytotoxicity was determined using the Brine Shrimp Lethality Test (BSLT), and anticancer activity was evaluated using the MTS assay. The results showed that the antioxidant activity of the extracts and solvent fractions of <em>Eleusine indica</em> had the highest average value for the n-hexane fraction (FNH) at 35.0%, ethyl acetate fraction (FEA) at 56.4%, aqueous fraction (FA) at 30.3%, and methanol extract (EM) at 32.5%. Among these, the highest value was obtained in FEA, while the lowest was obtained in FA. Based on the cytotoxicity test with the BSLT method, the ethyl acetate fraction had an LC50 value of 7.384 ppm, categorizing it as highly toxic. However, in cytotoxicity testing with HeLa cells, the IC50 value for the ethyl acetate fraction was 223.70 ppm, indicating that the ethyl acetate fraction was less active against HeLa cervical cancer cells.</p> <p>Keywords: <em>Eleusine indica</em>; belulang grass; cytotoxic; HeLa cells</p> Vanda Selvana Kamu, Max Revolta John Runtuwene, Jetlee Merentek, Harry Steven Julius Koleangan, Maureen Kumaunang Copyright (c) 2025 Vanda Selvana Kamu, Max Revolta John Runtuwene, Jetlee Merentek, Harry Steven Julius Koleangan, Maureen Kumaunang https://creativecommons.org/licenses/by-nc/4.0 https://ejournal.unsrat.ac.id/v3/index.php/JIS/article/view/58431 Sat, 15 Feb 2025 00:00:00 +0800