Uji daya hambat getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan Staphylococcus aureus
DOI:
https://doi.org/10.35790/eg.5.2.2017.17127Abstract
Abstract: Abscess is a chronic inflammatory condition formed by localized infections. In oral cavity abscess, the causal bacteria oftenly found is Staphylococcus aureus. Goroho banana (Musa acuminafe L.) is a typical plant in North Sulawesi. The sap of goroho banana peel contains phytochemicals inter alia flavonoids, saponins, and tannins. This study was aimed to find out whether the sap of goroho banana peel (Musa acuminafe L.) had an inhibitory effect on the growth of Staphylococcus aureus. This was an experimental laboratory study with a post-test only control group design. A modified Kirby-Bauer using paper disk was used as the analytical laboratory method. We used 100% goroho banana peel sap, clindamycin antibiotics as the positive control, and CMCs as the negative control. Staphylococcus aureus bacteria was obtained from the Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado. The results showed that the mean diameter of inhibition zones of goroho banana peel sap was 10.9 mm and was classified as strong inhibition. Conclusion: Goroho banana peel sap had a strong inhibitory effect on the growth of Staphylococcus aureus.
Keywords: goroho banana peel sap (Musa acuminafe L.), Staphylococcus aureus, inhibition zone
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Abstrak: Abses merupakan suatu kondisi inflamasi kronik yang terbentuk dari hasil infeksi yang terlokalisasi. Salah satu bakteri penyebab abses yang sering ditemukan pada rongga mulut ialah Staphylococcus aureus. Pisang goroho merupakan salah satu tanaman khas Sulawesi Utara. Getah kulit buahnya memiliki kandungan fitokimia seperti flavonoid, saponin, dan tanin. Penelitian ini bertujuan untuk mengetahui daya hambat getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian ini ialah eksperimental laboratorik dengan post test only control group design. Metode yang digunakan ialah modifikasi Kirby-Bauer dengan menggunakan paper disk. Konsentrasi getah buah pisang goroho yang digunakan yaitu 100%, kontrol positif menggunakan antibiotik klindamisin, dan kontrol negatif menggunakan CMC. Bakteri Staphylococcus aurues diambil dari stok bakteri murni Laboratorium Mikrobiologi Fakultas MIPA Unsrat Manado. Hasil penelitian menunjukkan diameter rerata zona hambat dari getah kulit buah pisang goroho yang terbentuk ialah 10,9 mm dan digolongkan sebagai zona hambat kuat. Simpulan: Getah kulit buah pisang goroho memiliki daya hambat yang kuat terhadap pertumbuhan Staphylococcus aureus.
Kata kunci: getah kulit buah pisang goroho (Musa acuminafe L.), Staphylococcus aureus, zona hambat
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