Efektivitas Ekstrak Daun Mangifera indica L. Menghambat Candida albicans pada Plat Resin Akrilik Heat-cured
DOI:
https://doi.org/10.35790/eg.v11i2.44596Abstract
Abstract: Heat-cured acrylic resin dentures are used to treat tooth loss, however, pores are easily formed on their surfaces which make it easier for Candida to adhere. Improper maintenance of dentures can cause overgrowth of Candida albicans and trigger denture stomatitis. This study aimed to determine the effectiveness of Mangifera indica L. extract on inhibition of Candida albicans on heat-cured acrylic resin plates. This was a true experimental laboratory study with a post-test only control group design. Samples were heat-cured acrylic resin plates contaminated with Candida albicans. The treatment groups were chlorhexidine gluconate 0.2%, aquades, and extract with concentrations of 65%, 75%, 85%, and 100%. Samples were immersed in the treatment groups for 15 minutes, followed by dilution and culture on SDA media, and then fungal colonies were observed. Data were analyzed with the Shapiro-Wilk normality test, the Levene's test for homogeneity, continued with the parametric one-way ANOVA and post hoc least significant difference tests. The results showed significant differences between the treatment groups (p<0.05). The percentage of the extract group with the lowest number of fungi was 75%, meanwhile the highest one was 65%. In conclusion, Mangifera indica L. leaf extract is effective in inhibiting the growth of Candida albicans on heat-cured acrylic resin plates with an optimal concentration of 75%.
Keywords: mango leaf extract (Mangifera indica L.); Candida albicans; heat-cured acrylic resin
Abstrak: Gigi tiruan resin akrilik heat-cured digunakan untuk mengatasi kehilangan gigi, namun sering terbentuk porus pada permukaannya yang mempermudah perlekatan Candida. Pemeliharaan gigi tiruan yang tidak tepat dapat menyebabkan pertumbuhan Candida albicans berlebihan dan memicu terjadinya denture stomatitis. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak daun mangga arum manis (Mangifera indica L.) dalam menghambat Candida albicans pada plat resin akrilik heat-cured. Jenis penelitian ialah true experimental laboratory dengan post test only control group design menggunakan sampel plat resin akrilik heat-cured yang dikontaminasi Candida albicans. Kelompok perlakuan yaitu chlorhexidine gluconate 0,2%, akuades, ekstrak konsentrasi 65%, 75%, 85%, dan 100%. Sampel direndam pada kelompok perlakuan selama 15 menit, dilanjutkan dengan tahap dilusi serta kultur pada media SDA, diakhiri dengan pengamatan koloni jamur. Analisis data menggunakan uji normalitas Shapiro Wilk, uji homogenitas Levene, dan dilanjutkan dengan uji parametrik One-Way ANOVA, dan Post Hoc Least Significant Difference. Hasil penelitian mendapat-kan perbedaan bermakna antar kelompok perlakuan (p<0,05). Kelompok ekstrak dengan angka jamur terendah ialah konsentrasi 75% dan yang tertinggi ialah konsentrasi 65%. Simpulan penelitian ini ialah ekstrak daun mangga arum manis (Mangifera indica L.) efektif dalam menghambat pertumbuhan Candida albicans pada plat resin akrilik heat-cured dengan konsentrasi optimal 75%.
Kata kunci: ekstrak daun Mangifera indica L.; Candida albicans; resin akrilik heat-cured
References
Laporan Nasional Riskesdas 2018. Jakarta Pusat: Kemenkes; 2018.
Pioh C, Siagian KV, Tendean L. Hubungan antara jumlah kehilangan gigi dengan status gizi pada lansia di Desa Kolongan Atas II Kecamatan Sonder. e-GiGi. 2018;6(2):143-150.
Sari KI, Dewi W, Jasrin TA, Sumarsongko T. Kebersihan gigi tiruan pada lansia, suatu tinjauan metode dan bahan. J Mater Kedokt Gigi. 2018;7(1):1.
Sundari I, Rahmayani L, Serpita D. Studi kekasaran permukaan antara resun akrilik heat cured dan termoplastik nilon yang direndam dalam kopi ulee kareng (Coffea robusta). Cakradonya Dent J. 2019;11(1):67–73.
Kusumawardani CDN, Chondro RT, Andrian I, Sari RP. Pengaruh penambahan hidroksiapatit terhadap porositas dan compressive strength basis resin akrilik heat-cured (Effect of hydroxyapatite addition towards porosity level and compressive strength of heat-cured acrylic resin base). J Kedokt Gigi Univ Padjadjaran. 2020;32(2):91.
Herawati M, Deviyanti S, Ferhad A. The antifungal potential of Stevia rebaudiana Bertoni leaf extract against Candida albicans. J Indones Dent Assoc. 2021;4(1):55–60.
Kumar SB. Chlorhexidine mouthwash- a review. J Pharm Sci Res. 2017;9(9):1450–2.
Ibrahim I, Jaya F, Luthfia P. Pengaruh lama perendaman dalam larutan chlorhexidine terhadap perubahan warna resin akrilik heat cured. J Mater Kedokt Gigi. 2016;5(1):7–14.
Zulkarnain M, Safitri E. Pengaruh perendaman basis gigi tiruan resin akrilik polimerisasi panas ( The effect of immersion denture base heat cured acrylic resin). DENTIKA Dent J. 2016;19(2):110–6.
Soeprapto A. Pedoman dan Tatalaksana Praktik Kedokteran Gigi. Yogyakarta: STPI Bina Insan Mulia; 2017. p 97.
Pratiwi N, Saputera D, Budiarti LY. Klorheksidin glukonat terhadap Candida albicans pada heat cured Akrilik. J Kedokt Gigi,. 2017;I(1):89–93.
Koesoemawati R. Antifungal effectiveness of soursop (Annonamuricata Linn) leaves extract againts Candida albicans on heat cured acrylic. Makassar Dent J. 2021;10(2):146–50.
Purwaningsih PP, Darmayasa IB, Astiti NPA. Elusidasi awal daya hambat ekstrak etanol daun ketapang (Terminalia catappa L.) terhadap pertumbuhan Staphylococcus aureus ATCC25923 penyebab gingivitis. Metamorf J Biol Sci. 2020;7(1):57.
Anggraeni VJ, Yulianti S, Panjaitan RS. Artikel review: Fitokimia dan Aktivitas antibakteri dari tanaman mangga (Mangifera indica L). Indones Nat Res Pharm J. 2020;5(2):102–13.
Ningsih DR, Zusfahair, Mantari D. Ekstrak daun mangga (Mangifera indica L.) sebagai antijamur terhadap jamur Candida albicans dan identifikasi golongan senyawanya. J Kim Ris. 2017;2(1):61.
Aboody MS Al, Mickymaray S. Anti-fungal efficacy and mechanisms of flavonoids. Antibiotics (Basel). 2020;9(2):45. Doi: 10.3390/antibiotics9020045.
Komala O, Yulianita, Siwi FR. Aktivitas antijamur ekstrak etanol 50% dan etanol 96% daun pacar kuku Lawsonia inermis L terhadap Trichophyton mentagrophytes. Ekologia. 2020;19(1):12–9.
Das M, Goswami S. Antifungal and antibacterial property of guava (Psidium guajava) leaf extract: role of phytochemicals. Int J Heal Sci Res [Internet]. 2019;9(2):39. Available from: www.ijhsr.org
Hsu H, Sheth CC, Veses V. Herbal extracts with antifungal activity against Candida albicans: a systematic review. Mini-Reviews Med Chem. 2020;21(1):90–117.
Jung KW, Chung MS, Bai HW, Chung BY, Lee S. Investigation of antifungal mechanisms of thymol in the human fungal pathogen, cryptococcus neoformans. Molecules. 2021;26(11):1–13.
Arifin Z, Khotimah S, Rahmayanti S. Aktivitas antijamur ekstrak etil asetat daun mangga bacang ( Mangifera foetida L.) terhadap Candida albicans secara in vitro. J Cerebellum. 2018;4(Agustus): 1106–19.
Bastian S, Rotinsulu H, Fatimawali. Uji Aktivitas antimikroba dari jamur laut yang berasosiasi dengan Spons Callyspongia sp. Pharmacon. 2018;7(3):311–20.
Cahyanto T, Fadillah A, Ulfa RA, Hasby RM, Kinasih I. Kadar mangiferin pada lima kultivar pucuk daun mangga (Mangifera indica L.). Al-Kauniyah J Biol. 2020;13(2):242–9.
Anggaraeni NMD. Perbedaan konsentrasi dan waktu perendaman ekstrak daun kedondong hutan (Spondias pinnata) terhadap pertumbuhan Candida albicans pada plat resin akrilik heat-cured [Skripsi]. Bali: Universitas Udayana; 2020.
Angelica S. Efektivitas ekstrak bawang merah (Allium ascalonicum Linn) dalam menghambat pertumbuhan Candida albicans pada basis gigi tiruan resin akrilik heat-cured [Skripsi]. Bali: Universitas Udayana; 2020.
Abdullah MT, Jubhari EH. Ekstrak tongkol jagung ( Zea mays L ) sebagai bahan desinfektan gigi tiruan terhadap Candida albicans. Makassar Dent J. 2016;5(3):82–6.
Christoper W, Natalia D, Rahmayanti S. Uji aktivitas antijamur ekstrak etanol umbi bawang Dayak (Eleutherine americana (Aubl.) Merr. Ex K. Heyne.) terhadap Trichophyton mentagrophytes secara in vitro. J Kesehat Andalas. 2018;6(3):685.
Sulistyawati D, Wiryosoendjojo K, Puspawati N. Uji aktivitas antijamur ekstrak etanolik daun dan daging buah berenuk (Crescentia cujete Linn.) terhadap Candida albicans ATCC 1023 (Anti-fungal activity test ethanolic extracts of calabash’s leaved and fruit meat. Biomedika. 2019;12(02):217–27.
Kumalasari E. Uji aktivitas ekstrak etanol 70% daun bawang Dayak (Eleutherinepalmifolia, (L.) Merr) terhadap pertumbuhan Candida albicans. J Insa Farm Indones. 2021;4(2):176–85.
Yanti N, Samingan, Mudatsir. Uji aktivitas antifungi ekstrak etanol gal manjakani (Quercus infectoria) terhadap Candida albicans. Jurnal Ilmiah Mahasiswa Pendidikan Biologi. 2016;1(1):1-9.
Maharani S. Pengaruh pemberian larutan ekstrak siwak (Salvadora persica) pada berbagai konsentrasi terhadap pertumbuhan Candida albicans. Jurnal PDGI. 2012:61(2):61-4.
Triana O, Prasetya F, Kuncoro H, Rijai L. Aktivitas antijamur ekstrak daun ketepeng Cina (Cassia alata L.). J Sains dan Kesehat. 2016;1(6):311–5.
Henaulu AH, Kaihena M. Potensi antibakteri ekstrak etanol daun kecipir (Psophocarpus Tetragonolobus (l.) Dc) terhadap pertumbuhan Escherichia Coli dan Staphylococcus Aureus in vitro. Biofaal J [Internet]. 2020;1(1):44–54. Available from: https://core.ac.uk/download/pdf/322568351.pdf
Allo MBR. Uji aktivitas antibakteri dari ekstrak air kulit buah pisang Ambon Lumut (Musa Acuminata Colla) terhadap pertumbuhan Staphylococcus aureus [Skripsi]. Yogyakarta: Universitas Sanata Dharma; 2016.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 IGA Kade I. Purbasari, Desak N. A. Susanti, Ni Ketut A. Lestarini
![Creative Commons License](http://i.creativecommons.org/l/by-nc/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
COPYRIGHT
Authors who publish with this journal agree to the following terms:
Authors hold their copyright and grant this journal the privilege of first publication, with the work simultaneously licensed under a Creative Commons Attribution License that permits others to impart the work with an acknowledgment of the work's origin and initial publication by this journal.
Authors can enter into separate or additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (for example, post it to an institutional repository or publish it in a book), with an acknowledgment of its underlying publication in this journal.
Authors are permitted and encouraged to post their work online (for example, in institutional repositories or on their website) as it can lead to productive exchanges, as well as earlier and greater citation of the published work (See The Effect of Open Access).