Uji Aktivitas Anti-Inflamasi Ekstrak Sabut Kelapa (Cocos nucifera L.) dengan Metode Stabilisasi Membran Sel Darah Merah
DOI:
https://doi.org/10.35790/eg.v13i1.55338Abstract
Abstract: Dental and oral care often causes injuries that can trigger an inflammatory reaction associated with feeling of discomfort. Drugs that can be given for the treatment of inflammation, namely corticosteroids of the glucocorticoid group and non-steroidal anti-inflammatories (NSAIDs). However, these drugs have side effects, therefore, alternatives with minimal toxicity that can be found in plants are preferrable. Coconut coir has the potential to be an anti-inflammatory drug because it contains flavonoids, tannins and saponins. This study aimed to determine the anti-inflammatory activity of coconut coir extract at concentrations of 50, 100, 150, 500, and 1000 ppm. This was a pure experimental study with a post-test-only control design using blood of rats weighing above 250 grams taken through the retro-orbital sinus. The samples were divided into seven groups, namely 50, 100, 150, 500, 1000 ppm, positive control, and negative control. The results showed that coconut coir extract contained alkaloids, flavonoids, tannins, saponins, and terpenoids. The percentages of inhibition of hemolysis were obtained at 50, 100, 150, 500, and 1000 ppm, namely 14.73%, 24.60%, 38.86%, 43.11%, and 50.39%. In conclusion, coconut coir extract (Cocos nucifera L.) has anti-inflammatory activity. Extract concentration of 1000 ppm has the highest anti-inflammatory activity.
Keywords: inflammation; coconut coir extract; stabilization of red blood cell membranes
Abstrak: Perawatan gigi dan mulut tidak jarang menimbulkan perlukaan yang dapat memicu reaksi inflamasi disertai rasa tidak nyaman. Golongan obat yang dapat diberikan untuk pengobatan inflamasi, yaitu kortikosteroid golongan glukokortikoid dan anti-inflamasi non-steroid (AINS). Namun, obat-obat tersebut memiliki efek samping sehingga dibutuhkan alternatif dengan toksisitas minimal yang dapat ditemukan pada tanaman. Sabut kelapa berpotensi menjadi obat anti-inflamasi karena mengandung flavonoid, tanin, dan saponin. Penelitian ini bertujuan untuk mengetahui aktivitas anti-inflamasi ekstrak sabut kelapa konsentrasi 50, 100, 150, 500, dan 1000 ppm. Penelitian ini merupakan eksperimental murni dengan post-test only control design menggunakan darah tikus yang diambil melalui sinus retro-orbitalis. Kriteria tikus ialah berat di atas 250 gram, yang dibagi menjadi tujuh kelompok, yaitu 50, 100, 150, 500, 1000 ppm, kontrol positif, dan kontrol negatif. Hasil penelitian mendapatkan bahwa ekstrak sabut kelapa mengandung senyawa alkaloid, flavonoid, tanin, saponin, dan terpenoid. Hasil persentase inhibisi hemolisis didapatkan pada 50, 100, 150, 500, dan 1000 ppm, yaitu 14,73%, 24,60%, 38,86%, 43,11%, 50,39%. Simpulan penelitian ini ialah ekstrak sabut kelapa (Cocos nucifera L.) memiliki aktivitas anti-inflamasi. Konsentrasi ekstrak 1000 ppm memiliki aktivitas anti inflamasi tertinggi.
Kata kunci: inflamasi; ekstrak sabut kelapa; stabilisasi membran sel darah merah
References
Sherwood L. Introduction to Human Physiology (8th ed). Australia: Brooks/Cole Learning, Cengage; 2013. p. 31, 440, 731.
Kumar V, Abbas AK, Aster JC, editors. Robbins Basic Pathology (10th ed). Philadelphia: Elsevier Inc; 2018. p. 57–71.
Barrett KE, Barman SM, Boitano S, Brooks HL. Buku Ajar Fisiologi Kedokteran (24th ed). New York: Mc Graw Hill Lange; 2012. p. 31, 39, 367.
Syarif A, Gayatri A, Estuningtyas A, Setiawati A, Muchtar A, Rosdiana DS, et al. Farmakologi dan Terapi (6th ed). Gunawan SG, Setiabudy R, Nafrialdi, Instiaty, editors. Jakarta: Badan Penerbit FK UI; 2016. p. 237–38.
Saifudin A. Senyawa Alam Metabolit Sekunder. Yogyakarta: Deepublish; 2014. p. 3.
Wulandari A, Bahri S, Mappiratu. Aktivitas antibakteri ekstrak etanol sabut kelapa (Cocos nucifera Linn) pada berbagai tingkat ketuaan. Kovalen J Ris Kim. 2019;4(3):276–84. Available from: http://jurnal.untad.ac.id/jurnal/index.php/kovalen/article/view/11854
Purwaningrum ND, Murtisiwi L, Pratimasari D. Uji aktivitas antibakteri ekstrak dan fraksi n-heksan, etil asetat dan air dari sabut kelapa muda (Cocos nucifera Linn) terhadap Escherichia coli ESBL (extended spectrum beta lactamase). J Ilm Ibnu Sina Ilmu Farm dan Kesehat. 2022;7(1):29–37. Doi: 10.36387/jiis.v7i1.773
Rethinam P. International scenario of coconut sector. In: Nampoothiri KUK, Krishnakumar V, Thampan PK, Nair MA, editors. The coconut Palm (Cocos nucifera L) - Research and Development Perspectives. Singapura: Springer; 2018. p. 21–2, 29.
Statistik BP. Produksi Tanaman Perkebunan (Ribu Ton), 2019-2021 [Internet]. 2021. Available from: https://www.bps.go.id/indicator/54/132/1/produksi-tanaman-perkebunan.html
Rinaldi S, Silva DO, Bello F, Alviano CS, Alviano DS, Matheus ME, et al. Characterization of the antinociceptive and anti-inflammatory activities from Cocos nucifera L. (Palmae). J Ethnopharmacol. 2009;122(3):541–6. doi: 10.1016/j.jep.2009.01.024.
Silva RR, de Silva DO, Fontes HR, Alviano CS, Fernandes PD, Alviano DS. Anti-inflammatory, antioxidant, and antimicrobial activities of Cocos nucifera var. typica. BMC Complement Altern Med [Internet]. 2013;13(1):107. Available from: http://www.biomedcentral.com/1472-6882/13/ 107 RESEARCH ARTICLE
Sari Y, Yulis PAR, Putri II, Putri AM, Anggraini S. Penentuan kandungan metabolit sekunder ekstrak etanol sabut kelapa muda ( Cocos nucifera L.) secara kualitatif. J Res Educ Chem. 2021;3(2):113–21. Doi: 10.25299/jrec.2021.vol3(2).7579
Hall JE. Guyton and Hall Textbook of Medical Physiology (12th ed). Gruliow R, Stingelin L, editors. Philadelphia: Saunders, Elsevier Inc.; 2011. p. 291–92, 426.
Thangaraj P. Pharmacological assay of lant-based natural products. Rainsford KD, editor. Progress in Drug Research. Switzerland: Springer; 2016. p. 103–10.
Chiavaroli A, Goci E, Recinella L, editors. Chronic oxidative stress and inflammation-related diseases – the protective potential of medicinal plants and natural products. Lausanne: Frontiers Media SA; 2022. p. 138.
Sofiana MSJ, Safitri I, Warsidah W, Helena S, Nurdiansyah SI. Antioxidant and anti-inflammatory activities from ethanol extract of Eucheuma cottonii from lemukutan island waters west kalimantan. Saintek Perikan Indones J Fish Sci Technol. 2021;17(4):247–53. Doi: 10.14710/ijfst. 17.4.247-253
Fadilaturahmah, Syukri F, Afriani Y, Santoso P. Anti-Inflammatory Effects of Miang Bean Leaves (Mucuna pruriens). J Trop Pharm Chem [Internet]. 2022;6(1):76–83. Available from: https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/284
Oyedapo O. Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. Int J Plant Physiol Biochem [Internet]. 2010;2(October):46–51. Available from: http://www.academicjournals.org/ijppb/PDF/PDF 2010/Oct/Oyedapo et al.pdf
Fristiohady A, Wahyuni, Malik F, La Ode Purnama MJ, Sadarun BSI. Anti-inflammatory activity of marine sponge Callyspongia Sp. and its acute toxicity. Asian J Pharm Clin Res. 2019;12(12):97–100. Doi: http://dx.doi.org/10.22159/ajpcr.2019.v12i12.34737
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