Identifikasi Bakteri pada Permukaan Kerja sebelum Tindakan Ekstraksi Gigi
DOI:
https://doi.org/10.35790/eg.v14i1.62670Keywords:
healthcare associated infections; bakteri; dental unitAbstract
Abstract: A hospital is a building where sick people are treated or where healthcare services are provided, but hospitals can also become sources of various diseases, known as nosocomial infections or Healthcare Associated Infections (HAIs). Work surfaces on dental units can become vectors for the transfer of microorganisms during various procedures or treatments performed by dentists, such as tooth extraction. This study aimed to identify bacteria on work surfaces before tooth extraction procedures at RSGM Universitas Sam Ratulangi (Unsrat). This was a descriptive and analytical study with a cross-sectional design. Samples were taken using purposive sampling techniques according to inclusion criteria on three types of work surfaces: headrests, light handles, and control panels of work surfaces on four dental units used for tooth extraction procedures. Based on swab examination results, three types of bacteria were identified on work surfaces before tooth extraction procedures: Streptococcus sp. (66.7%), Staphylococcus sp. (25%), and Klebsiella sp. (8,3%). In conclusion, bacteria predominated by Streptococcus sp. were identified on dental unit work surfaces before tooth extraction procedures.
Keywords: healthcare associated infections; bacteria; dental unit
Abstrak: Rumah sakit dapat menjadi sumber berbagai penyakit, yang dikenal sebagai penyakit infeksi nosokomial atau healthcare associated infections (HAIs). Permukaan kerja pada dental unit menjadi salah satu perantara perpindahan mikroorganisme saat dilakukan berbagai prosedur perawatan atau tindakan oleh dokter gigi; salah satunya ialah saat melakukan tindakan ekstraksi gigi. Penelitian ini bertujuan untuk mengidentifikasi bakteri pada permukaan kerja sebelum tindakan ekstraksi gigi di RSGM Universitas Sam Ratulangi. Jenis penelitian ialah analisis deskriptif dengan desain potong lintang. Sampel penelitian diambil berdasarkan teknik purposive sampling sesuai dengan kriteria inklusi pada tiga jenis permukaan kerja, yakni sandaran kepala, light handle, dan panel kontrol dari permukaan kerja pada empat dental unit yang digunakan untuk tindakan ekstraksi gigi. Hasil pemeriksaan swab teridentifikasi tiga jenis bakteri pada permukaan kerja sebelum tindakan ekstraksi gigi, yakni Streptococcus sp. (66,7%), Staphylococcus sp. (25%), dan Klebsiella sp. (8,3%). Simpulan penelitian ini yaitu teridentifikasi adanya bakteri terutama Streptococcus sp. pada permukaan kerja dental unit sebelum tindakan ekstraksi gigi.
Kata kunci: healthcare associated infections; bakteri; dental unit
References
1. Kementerian Kesehatan. Rencana Strategis Bisnis (RSB) RSTC 2015-2019. 2015 [cited 2023 Sep 10]. p. 86. Available from: https://b2p2vrp.litbang.kemkes.go.id/asset/file/renstra_rev_1_april_2017_(2015-2019).pdf
2. Yobee FEA, Rares FES, Homenta H. Isolasi dan identifikasi bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di Irina F ruangan intermediate care (IMC) Bagian Neurologi RSUP Prof. Dr. R. D. Kandou Manado. eBiomedik. 2017;5(1). Doi: 10.35790/ebm.5.1.2017.14803
3. Goh WLP, Marbawi H, Goh SM, Abdul AAK, Gansau JA. The prevalence of hospital-acquired infections in Southeast Asia (1990-2022). J Infect Dev Ctries. 2023;17(2):139–46. Doi: 10.3855/JIDC.17135
4. Khan HA, Baig FK, Mehboob R. Nosocomial infections: epidemiology, prevention, control and surveillance. Asian Pac J Trop Biomed. 2017;7(5):478–82. Doi: http://dx.doi.org/10.1016/j.apjtb.2017.01.019
5. Safira AR, Inge D. Infections Prevention and Control (IPC) Programs in hospitals. J Heal Sci Prev. 2021;5(1):23–32. Doi: http://doi.org/10.29080/jhsp.v5i1.39
6. Alrebish SA, Yusufoglu HS, Alotibi RF, Abdulkhalik NS, Ahmed NJ, Khan AH. Epidemiology of healthcare-associated infections and adherence to the HAI prevention strategies. Healthc. 2023;11(1):1–9. Doi: https://doi.org/10.3390/healthcare11010063
7. Lumunon NP, Wowor VNS, Pangemanan DHC. Pencegahan dan pengendalian infeksi silang pada tindakan ekstraksi gigi di Poli Gigi Puskesmas Kakaskasen Tomohon. e-GiGi. 2019;7(1):34–43. Doi: 10.35790/eg.7.1.2019.23311
8. Rosmiati, Wiradona I, Supriyana. Influence of diabetes mellitus status to the severity of periodontal disease and saliva pH on diabetes mellitus patient at Puskesmas Pudak Payung Semarang. J Appl Heal Manag Technol. 2023;5(2):34–8. Available from: http://ejournal.poltekkes-smg.ac.id/ojs/index.php/JAHM
9. Peraturan Menteri Kesehatan Nomor 27 Tahun 2017 tentang Pedoman Pencegahan dan Pengendalian Infeksi di Fasilitas Pelayanan Kesehatan [cited 2023 Oct 22].
10. Abusalim GS. Prevalence and investigations of bacterial contamination in dental healthcare associated environment. J King Saud Univ-Sci. 2022;34(6):102153. Doi: https://doi.org/10.1016/j.jksus.2022.102153
11. Sachwiver B, Surya LS, Elianora D. Identifikasi bakteri pada 3 permukaan dental unit (bowl rinse, dental chair, instrument table) di RSGM Universitas Baiturrahmah tahun 2018. B-Dent J Kedokt Gigi Univ Baiturrahmah. 2019;5(1):65–71. Doi: 10.33854/jbdjbd.140
12. Suleh MM, Wowor VNS, Mintjelungan CN. Pencegahan dan pengendalian infeksi silang pada tindakan ekstraksi gigi di Rumah Sakit Gigi dan Mulut PSPDG FK Unsrat. e-GiGi. 2015;3(2). Doi: 10.35790/eg.3.2.2015.10482
13. Chris H. Miller BAMS. Infection control and management of hazardous materials for the dental team. Elsevier Health Sciences; 2013. p. 120-52.
14.Sriraman P, Neelakantan P. Asepsis in operative dentistry and endodontics. Int J Public Heal Sci. 2014;3(1):1–6. Doi: 10.11591/ijphs.v3i1.5297
15.Deulkar S, Singh S, Tiwari D. Isolation of selected possible aerobic bacterial pathogens from dental environmental surfaces after use of disinfectants - a case study at a public dental clinic, in KwaZulu-Natal. South African Dent J. 2020;75(5):241–6. Doi: 10.17159/2519-0105/2020/v75no5a2
16. Makvandi P, Song H, Yiu CKY, Sartorius R, Zare EN, Rabiee N, et al. Bioengineered materials with selective antimicrobial toxicity in biomedicine. Mil Med Res. 2023;10(1):1–20. Doi: 10.1186/s40779-023-00443-1
17. Reichel M, Schlicht A, Ostermeyer C, Kampf G. Efficacy of surface disinfectant cleaners against emerging highly resistant Gram-negative bacteria. BMC Infect Dis. 2014;14(1):1–8. Doi: 10.1186/1471-2334-14-292
18. Shenkute D, Asfaw T. Streptococcus mutans dental caries among patients attending Debre Berhan referral hospital, Ethiopia. J Bacteriol Parasitol. 2019;10(1):49–55. Doi: 10.35248/2155-9597.1000350
19. Syafira A, Noer M, Syafrawati S. Identify and test the resistance of bacteria on dental unit surfaces in dental clinic of Dentistry Faculty [Skripsi]. Padang: Universitas Andalas; 2017. Doi: http://dx.doi.org/10.7454/uiphm.v1i0.29
20. Peng X, Cheng L, You Y, Tang C, Ren B, Li Y, et al. Oral microbiota in human systematic diseases. Int J Oral Sci. 2022;14(1):1–11. Doi: 10.1038/s41368-022-00163-7
21. Rotzetter J, Le ND, Leib FL, Leib SL, Gsell M, Feldmann J, et al. Determining the efficacy of chemicals for the inactivation of liquid waste containing gram-positive bacteria of risk group 2. Appl Biosaf. 2024;00(00):1–12. Doi: 10.1089/apb.2023.0012
22. Holt JG, Krieg NR, Sneath PHA, Staley JT WS. Bergey’s Manual of Determinative Bacteriology. Baltimore: The Williams & Wilkins Company; 1994. p. 532.
23. Waworuntu MC, Rares FES, Homenta H. Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruang neonatal intensive care unit (NICU) RSAD Robert Wolter Mongisidi Manado. eBiomedik. 2016;4(2). Doi 10.35790/ebm.4.2.2016.14610
24. Shamloo N, Mir M, Goudarzi H, Namdari M, Rafiei F, Hakemi-Vala M. Microbial contamination of tray, light handle, and dental chair handles before and after disinfection with Deconex. Journal of Dental School. 2021;39(2):57-60. Doi: https://doi.org/10.22037/jds.v39i2.35841
25. Zusandy AK, Sommeng F, Musa IM, Aryanti, Amir SP. Bakteri penyebab infeksi nosokomial di ruang rawat inap. Fakumi Med J J Mhs Kedokt. 2021;1(2):97–103. Doi: 10.33096/fmj.v1i2.83
26. Tsuzukibashi O, Fukatsu A, Yamamoto H, Takahashi Y, Usuda K, Fuchigami M, et al. Isolation and identification methods for oral Klebsiella pneumoniae involved in onset of inflammatory bowel disease. Open J Stomatol. 2023;13(07):197–211. Doi: 10.4236/ojst.2023.137017
27. Gholami D, Emruzi Z, Noori AR, Aminzadeh S. Advances in bacterial identification and characterization: methods and applications. Adv Res Microb Metab Technol. 2019;2(2019):119–36. Available from: http://armmt.irost.ir
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