Analisis Ekspresi MMP-9 dan Hitung Fibroblas sebagai Marker Penyembuhan Ulkus Traumatikus dengan St. John’s Wort (Hypericum perforatum) Topikal pada Tikus Diabetes Melitus
DOI:
https://doi.org/10.35790/eg.v14i1.63070Keywords:
St. John’s wort; ulkus traumatik; diabetes melitus; fibroblas; matriks metaloproteinase–9Abstract
Abstract: Diabetes mellitus impairs oral ulcer healing by reducing fibroblast proliferation and increasing MMP-9 expression. St. John’s Wort exhibits anti-inflammatory activity and supports tissue regeneration. This study aimed to evaluate the effect of topical St. John’s Wort on MMP-9 expression and fibroblast count in the oral mucosa of diabetic rats with traumatic ulcers. Fifteen Wistar rats induced with alloxan to establish diabetes were subjected to ulcer creation on the labial mucosa. Wistar rats were divided into three groups, namely traetment group (topical St. John’s Wort oil), positive control group (Aloclair gel), and negative control group. Tissue samples were collected on day-7 for histological (HE stain) and immunohistochemical (IHC) examination. Data were analyzed using one-way ANOVA with a significance level of p<0.05. The results demonstrated that topical St. John’s Wort significantly increased fibroblast count and reduced MMP-9 expression. In conclusion, topical St. John’s Wort has the potential to accelerate traumatic ulcer healing under diabetic conditions
Keywords: St. John’s wort; traumatic ulcer; diabetes mellitus; fibroblast; matrix metalloproteinases–9
Abstrak: Diabetes melitus menghambat penyembuhan ulkus mulut melalui penurunan proliferasi fibroblas dan peningkatan MMP-9. St. John’s wort diketahui memiliki aktivitas antiinflamasi dan mendukung proses regenerasi jaringan. Penelitian ini bertujuan mengevaluasi efek St. John’s wort topikal terhadap ekspresi MMP-9 dan jumlah fibroblas pada mukosa mulut tikus diabetes dengan ulkus traumatik. Lima belas ekor tikus wistar diinduksi diabetes dengan alloxan, dibuat ulkus pada mukosa labial, dan selanjutnya dibagi menjadi tiga kelompok, yaitu kelompok perlakuan (St. John’s Wort oil topikal), kontrol positif (Aloclair gel), dan kontrol negatif (tidak diberikan apapun). Jaringan mukosa ulkus diambil pada hari ke-7 untuk pemeriksaan histologik (pulasan HE) dan imunohistokimia (IHC). Analisis data menggunakan one-way ANOVA dengan taraf signifikansi p<0,05. Hasil penelitian mendapatkan bahwa pemberian St. John’s wort meningkatkan jumlah fibroblas dan menurunkan ekspresi MMP-9 secara bermakna. Simpulan penelitian ini ialah St. John’s wort topikal berpotensi mempercepat penyembuhan ulkus traumatikus pada kondisi diabetes melitus.
Kata kunci: St. John’s wort; ulkus traumatik; diabetes melitus; fibroblas; matriks metaloproteinase–9
References
1.Subandi E, Sanjaya KA. Efektifitas modern dressing terhadap proses penyembuhan luka diabetes melitus tipe 2. Jurnal Kesehatan.2020;10(1):1273–84. Doi: https://doi.org/10.38165/jk.v10i1.7
2. Sarifah L, Jannah R, Sari DRT. Efek hipoglikemia formula herbal antidiabetes pada tikus diabetes yang diinduksi alloxan. JPSP. 2024;4(1):84–94. Doi: https://doi.org/10.23971/jpsp.v4i1.8010
3. Suparno NR, Rizqinavia GA, Putri NAP. Oral mucoadhesive patch of green betle leaf, areca nut, and gambier can reduce the size of traumatic ulcer lesion. Odonto. 2023;10(1):100. Doi: https://doi.org/10.30659/ odj.10.1.100-107
4. Pratama D, Wibawa IK, Patrianef P. Analisis hubungan polimorfisme gen matrix metalloproteinase-9 (MMP-9) dengan penyakit ulkus diabetik pada penderita diabetes mellitus tipe 2 di RSCM. Jurnal Ilmu Bedah Indoenesia. 2020;47(1):57–68. Doi: https://doi.org/10.46800/jibi-ikabi.v47i1.20
5. Purnamasari YW, Rahmah NA, Ernanda H, Sari W. Effect of aloe vera gel and sodium metabisulphite on expression of fibroblast growth factor in incision wound of rats. World Vet J. 2024;14(3):311–7. Doi: https://doi.org/10.54203/scil.2024.wvj37
6. Bhandari N, Dhudum B. Effect of aloe vera pulp on pressure ulcers among bed ridden patients. Journal of Pharmaceutical Negative Results. 2022;13(8):1227–35. https://doi.org/10.47750/pnr.2022.13.S08.152
7. Canbaz Y, Karakol P, Erten R, Mercantepe T, Alp HH, Canbaz ET, et al. Comparison of the effectiveness of thymoquinone, St. John wort oil and silver sulfadiazine in experimental burn wounds. Journal of Burn Care & Research. 2025;46(2):400-10. Doi: https://doi.org/10.1093/jbcr/irae174
8. Çobanoğlu A. Effect of St. John’s Wort on wound healing. ACAM. 2018;2(5):178-9. https://doi.org/10.31031/ACAM.2018.02.000548
9. Yücel A, Kan Y, Yesilada E, Akın O. Effect of St. John’s wort (Hypericum perforatum) oily extract for the care and treatment of pressure sores; a case report. Journal of Ethnopharmacology. 2017;196:236–41. Doi: https://doi.org/10.1016/j.jep.2016.12.030
10. Aksu AN, Bilgic T, Kayhan CK, Isik Saglam ZM, Caf N. Partial healing effects of St. John’s wort oil on the rat excisional wound model. Marmara Medical Journal. 2022;35(3):281-7, 31.10.2022. Doi: https://doi.org/10.5472/marumj.1186858
11. Lin B-Y, Kamata S-I, Ahmad Fauzi MF, Ahmad WSHMW. New automatic allred scoring for breast cancer nuclei detection and scoring from ER-IHC stained images. 2024 IEEE 8th International Conference on Signal and Image Processing Applications (ICSIPA). Kuala Lumpur, Malaysia: IEEE; 2024. p. 1–6. Doi: https://doi.org/10.1109/ICSIPA62061.2024.10686193
12.Rif’at ID, Hasneli NY, Indriati G. Gambaran komplikasi diabetes melitus pada penderita diabetes melitus. JKP. 2023;11(1):52–69. Doi: https://doi.org/10.33650/jkp.v11i1.5540
13. Rahakratat AMN, Astuti RA, Irwandi I. Efektivitas ekstrak daun binahong (Anredera cardifolia L) pada penyembuhan luka akut mencit jantan (Mus musculus). JKT. 2024;5(4):13193–201. Doi: https://doi.org/10.31004/jkt.v5i4.38212
14. Hidayat LH, Hadi P, Radithia D. Pengaruh pemberian ZnSO4 1% topikal terhadap ekspresi MMP-9 dan jumlah fibroblas pada penyembuhan ulkus traumatikus tikus Wistar diabetes melitus. Eprodenta. 2018;02(02):163–71. Doi: https://doi.org/10.21776/ub.eprodenta.2018.002.02.4
15. Temel M, Atılgan N, Aydemir S, Dokuyucu R. Comparison of St. John’s wort oil and thiocilline ointment on wound healing in a diabetic rat model. Journal of Medicine and Palliative Care. 2024;5(4):208–13. Doi: Doi: https://doi.org/10.47582/jompac.1515587
16. Altıparmak M, Eskitaşçıoğlu T. Comparison of systemic and topical Hypericum perforatum on diabetic surgical wounds. Journal of Investigative Surgery. 2018;31(1):29–37. Doi: https://doi.org/10.1080/ 08941939.2016.1272654
17. Khofifah SD, Suparno NR, Sari M, Vernanda MR, Azmi NMIT. The effectiveness of ethanolic extract of Centella asiatica (L.) on healing minor recurrent aphthous stomatitis in Wistar male rats (Rattus norvegicus). Advances in Health Sciences Research. Surakarta: Atlantis Press; 2022. Doi: https://doi.org/10.2991/ahsr.k.220403.007
18. Nobakht SZ, Akaberi M, Mohammadpour A, Tafazoli MA, Emami A. Hypericum perforatum: Traditional uses, clinical trials, and drug interactions. Iran J Basic Med Sci. 2022;25(9):1045-58. Doi: https://doi.org/10.22038/ijbms.2022.65112.14338
19. Bigliardi P, Langer S, Cruz JJ, Kim SW, Nair H, Srisawasdi G. An Asian perspective on povidoneiodine in wound healing. Dermatology. 2017;233(2–3):223–33. Doi: https://doi.org/10.1159/000479150
20. Freeman C, Duan E, Kessler J. Molecular iodine is not responsible for cytotoxicity in iodophors. J Hospital Inf. 2022;122:194–202. Doi: https://doi.org/10.1016/j.jhin.2022.01.015
21. Liu C, Cui Y, Pi F, Cheng Y, Guo Y, Qian H. Extraction, Purification, Structural Characteristics, Biological Activities and Pharmacological Applications of Acemannan, a Polysaccharide from Aloe vera: A Review. Molecules. 2019;24(8):1554. Doi: https://doi.org/10.3390/molecules24081554
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