Antimicrobial Effects of Cassava Peel and Leaves against Oral Pathogens
DOI:
https://doi.org/10.35790/eg.v14i2.65511Keywords:
antibacterial; cassava peel; cassava leaves; oral pathogens; phytochemicalsAbstract
Abstract: Cassava peel and leaves (Manihot esculenta Crantz) are agro-waste materials rich in secondary metabolites with potential antimicrobial properties. Research exploring their effects against oral pathogens remains limited. This study aimed to evaluate the antibacterial and antifungal activity of cassava peel and leaf extracts against Staphylococcus aureus, Streptococcus mutans, and Candida albicans. This was a laboratory and experimental study. Samples of cassava leaves, outer and inner young peel (six months), outer and inner mature peel (10 months) were dried, powdered, and subjected to ethanol maceration. Phytochemical screening assessed flavonoids, alkaloids, saponins, tannins, quinones, steroids, and triterpenoids. Antimicrobial activity was tested using the microdiffusion well method, and inhibition zones were measured in millimeters. Positive controls were chloramphenicol (for bacteria) and nystatin (for fungi). Negative control was sterile aquadest. The results showed that leaf extract produced inhibition zones of 8.90 mm (S. aureus), 6.00 mm (S. mutans and C. albicans). Peel extracts showed varying activity, with outer young peel (6 months) yielding 7.25 mm (S. aureus), 6 mm (S. mutans and C. albicans) and inner young peel (6 months) yielding 8.74 mm (S. aureus), 35.27 mm (S. mutans), 6 mm (C. albicans) while outer mature peel (10 months) yielding 6.13 mm (S. aureus), 6.00 mm (S. mutans and C. albicans) and inner mature peel (10 months) yielding 10.44 mm (S. aureus), 55.36 mm (S. mutans), 6 mm (C. albicans). Phytochemical screening confirmed the presence of flavonoids, tannins, and saponins in most of cassava peel and leave samples. In conclusion, cassava peel and leaf extracts exhibit antimicrobial activity against major oral pathogens, with inner mature peels (10 months) showing the strongest effect against Streptococcus mutans. These results support their potential use as natural antimicrobial agents in herbal oral care formulations.
Keywords: antibacterial; cassava peel; cassava leaves; oral pathogens; phytochemicals
References
1. Ubalua AO. Cassava wastes: treatment options and value addition alternatives. Afr J Biotechnol. 2007;6(18):2065-73. Doi: https://doi.org/10.5897/AJB2007.000-2319
2. Fachriyah E, Haryanto IB, Kusrini D, Sarjono PR, Ngadiwiyana N. Antioxidant activity of flavonoids from cassava leaves (Manihot esculenta Crantz). Jurnal Kimia Sains dan Aplikasi. 2023;26(1):10-8. Doi: https://doi.org/10.14710/jksa.26.1.10-18
3. Olaniyan OP, Ajayi EIO. Phytochemicals and in vitro anti-apoptotic properties of ethanol and hot water extracts of cassava (Manihot esculenta Crantz) peel biogas slurry following anaerobic degradation. Clinical Phytoscience. 2021;7:77. Doi: https://doi.org/10.1186/s40816-021-00311-2
4. Czerkas K, Olchowik-Grabarek E, Łomanowska M, Abdulladjanova N, Sękowski S. Antibacterial activity of plant polyphenols belonging to the tannins against Streptococcus mutans—Potential against dental caries. Molecules. 2024;29(4):879. Doi: https://doi.org/10.3390/molecules29040879
5. Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol. 2012;23(2):174-81. Doi: https://doi.org/10.1016/j.copbio.2011.08.007
6. Jeon JG, Rosalen PL, Falsetta ML, Koo H. Natural products in caries research: current (limited) knowledge, challenges and future perspective. Caries Res. 2011;45(3):243–63. Doi: https://doi.org/ 10.1159/000327250
7. Dewanti RI, Ciptaningtyas VR, Halleyantoro R, Wibobo GW. Comparison of antibacterial effectiveness of herbal toothpaste and fluoride on the growth of Streptococcus mutans. Jurnal Kedokteran Diponegoro. 2022;11(5):253-6. Doi: https://doi.org/10.14710/dmj.v11i5.35640
8. Moghadamtousi SZ, Kadir HA, Hassandarvish P, Tajik H, Abubakar S, Zandi K. A review on antibacterial, antiviral, and antifungal activity of curcumin. BioMed Res Int. 2014;2014:186864. Doi: https://doi.org/10.1155/2014/186864
9. Raut JS, Karuppayil SM. A status review on the medicinal properties of essential oils. Industrial Crops and Products. 2014;62:250-64. Doi: https://doi.org/10.1016/j.indcrop.2014.05.055
10. de Castro RD, de Souza TM, Bezerra LM, Ferreira GL, Costa EM, Cavalcanti AL. Antifungal activity and mode of action of thymol and its synergism with nystatin against Candida species involved with infections in the oral cavity: an in vitro study. BMC Complementary and Alternative Medicine. 2015;15:417. Doi: https://doi.org/10.1186/s12906-015-0947-2
11. Silva LN, Zimmer KR, Macedo AJ, Trentin DS. Plant natural products targeting bacterial virulence factors. Chemical Reviews. 2016;116(16):9162–236. Doi: https://doi.org/10.1021/acs.chemrev.6b00184
12. Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current Medicinal Chemistry. 2015;22(1):132-49. Doi: https://doi.org/10.2174/ 0929867321666140916113443
13. Pamadya S, Azhari A, Firman RA. Correlation of cervical vertebral maturity and teeth calcification stages in children with cleft lip and palate. Majalah Kedokteran Gigi Indonesia. 2018;4(3):167-71. Doi: https://doi.org/10.22146/majkedgiind.31875
14. Puspita S, Soesatyo MH, Sunarintyas S, Mulyawati E. The fibroin (Bombyx mori L.) is cytocompatible with human primary pulp cells. AIP Conf Proc. 2019;2099(1):020018. Doi: https://doi.org/10.1063/1.5098423
15. Siqueira JF Jr, Fouad AF. Endodontic microbiology. In: Hargreaves KM, Berman LH, editors. Endodontics: Principles and Practice. 5th ed. St. Louis (MO): Elsevier; 2016. p. 37-52.
16. Belal AR, Cooper SM, Khan NA. Corporate environmental responsibility and accountability: What chance in vulnerable Bangladesh? Critical Perspectives on Accounting. 2015;33:44–58. Doi: https://doi.org/10.1016/j.cpa.2015.01.005
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