Uji Aktivitas Antibakteri Fraksi Daun Sesewanua (Clerodendron squamatum Vahl.) Terhadap Bakteri Streptococcus mutans dan Escherichia coli

Abstract

Telah dilakukan penelitian mengenai kandungan total fenolik, flavonoid dan tanin terkondensasi dari hasil pemisahan kromatografi kolom dan uji aktivitas antibakteri fraksi daun sesewanua terhadap bakteri Streptococcus mutans dan escherecia coli. Pemisahan kromatografi kolom dimulai dengan proses ekstraksi maserasi menggunakan pelarut metanol. Hasil penelitian menunjukkan bahwa kandungan total fenolik tertinggi pada fraksi metanol yaitu 135,497 mg/g, kandungan total flavonoid tertinggi pada fraksi etil asetat yaitu 102,647 mg/g dan kandungan total tanin terkondensasi tertinggi pada fraksi metanol yaitu 53,543 mg/g.  Uji aktivitas antibakteri menggunakan konsentrasi fraksi 40%, 60% dan 80% dilakukan sebanyak tiga kali pengulangan. Larutan DMSO 10% digunakan sebagai kontrol negatif dan kloramfenikol sebagai kontrol positif. Hasil menunjukkan bahwa fraksi n-heksana tidak memiliki zona hambat sedangkan fraksi etil asetat dan fraksi metanol daun sesewanua dapat menghambat bakteri Streptococcus mutans dan Escherecia coli. Konsentrasi fraksi 80% menunjukkan zona hambat yang paling besar

Research has been conducted on the total content of phenolics, flavonoids and condensed tannins from the results of column chromatography separation. Antibacterial activity test of leaf fractions was also performed against Streptococcus mutans and Escherecia coli. The separation of column chromatography was initialized with the process of maceration using methanol as solvents. The highest total phenolic content obtained in the methanol fraction was 135.497 mg/g, the highest total flavonoid content obtained in the ethyl acetate fraction was 102.647 mg/g and the highest total condensed tannin content in the methanol fraction was 53.543 mg/g. Antibacterial activity test was done triplicates using the 40%, 60% and 80% fraction. DMSO 10% was apllied as the negative control and chloramphenicol as the positive control. The results showed that the n-hexane fraction did not have an inhibition zone while the ethyl acetate fraction and the methanol fraction of the leaves can inhibit Streptococcus mutans and Escherecia coli. Moreover, 80% fraction showed the largest inhibition zone.