ISOLASI DAN UJI AKTIVITAS ANTI BAKTERI DARI JAMUR SIMBION DARI TERIPANG (Holothuroidea sp.) YANG DIAMBIL DI PERAIRAN KELURAHAN MOLAS KECAMATAN BUNAKEN PROVINSI SULAWESI UTARA

Authors

  • Epsan J Mamangkey FPIK UNSRAT
  • Fitje Losung FPIK UNSRAT
  • Robert A Bara FPIK UNSRAT
  • Ping Astony Angmalisang FPIK Unsrat
  • Natalie DC Rumampuk FPIK UNSRAT
  • Reiny Tumbol FPIK UNSRAT

DOI:

https://doi.org/10.35800/jplt.10.2.2022.42017

Abstract

Sea cucumbers are one of the marine biotas which consist of high nutritional and protein content. One bioactive compound in sea cucumbers is triterpenoid with antifungal and anti-bacterial properties. This study aimed to obtain fungal symbionts derived from marine cucumbers from Molas Waters, North Sulawesi continued by extraction and testing their antibacterial property towards bacterial strains Escherichia coli and Staphylococcus aureus. The antibacterial activity test was carried out by using the Kirby-Bauer disc diffusion method. In the results, we found 3 species of sea cucumbers from Molas Waters, e. g. Holothuria atra, Bohadschia argus and Holothuria scabra. From these species, three isolates of fungal symbionts were isolated and tested for their antibacterial property. These three extracts array antibacterial activity against E. coli and S.aureus. The most potent antibacterial activity was found in the extract of fungal symbiont from H. atra with an average of 29.3 mm against E.coli and 28.0 mm against S.aureus.

 

Keywords: Sea cucumber, antibacterial activity test, Escherichia coli, Staphylococcus aureus, fungal symbiont.

Author Biographies

Epsan J Mamangkey, FPIK UNSRAT

Mahasiswa Program Studi Ilmu Kelautan

Fitje Losung, FPIK UNSRAT

Dosen FPIK UNSRAT PS Ilmu Kelautan

Robert A Bara, FPIK UNSRAT

Dosen Program Studi Ilmu Kelautan

Ping Astony Angmalisang, FPIK Unsrat

Dosen PS Ilmu Kelautan

Natalie DC Rumampuk, FPIK UNSRAT

Dosen PS Ilmu Kelautan

Reiny Tumbol, FPIK UNSRAT

Dosen PS Budidaya Perairan

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How to Cite

Mamangkey, E. J., Losung, F., Bara, R. A., Angmalisang, P. A., Rumampuk, N. D., & Tumbol, R. (2022). ISOLASI DAN UJI AKTIVITAS ANTI BAKTERI DARI JAMUR SIMBION DARI TERIPANG (Holothuroidea sp.) YANG DIAMBIL DI PERAIRAN KELURAHAN MOLAS KECAMATAN BUNAKEN PROVINSI SULAWESI UTARA. JURNAL PESISIR DAN LAUT TROPIS, 10(2), 79–88. https://doi.org/10.35800/jplt.10.2.2022.42017