UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL SPONS Stylissa carteri DARI PULAU MENTEHAGE MINAHASA UTARA
DOI:
https://doi.org/10.35799/pha.11.2022.39142Abstract
ABSTRACT
Sponges are one of the animals from the phylum porifera and are marine invertebrates that live in coral reef ecosystems. Sponges are multi-cellular marine biota whose tissue and organ functions are very simple. Sponge Stylissa carteri can produce secondary metabolites from metabolic processes in the cells that exist in the body. This study aims to determine the antioxidant activity of the ethanolic extract of sponge Stylissa carteri from Mantehage Island, North Minahasa. Sponge Stylissa carteri was extracted by maceration method using ethanol as solvent. The antioxidant activity test was carried out using the DPPH method (1,1-diphenyl-2- picrylhydrazyl), which was measured by means of a UV-Vis spectrophotometer. The results of the study showed that the ethanolic extract of the sponge Stylissa carteri performed the antioxidant activity at each concentration, at a concentration of 0,5 mg/L (77,7 %), at a concentration 0,6 mg/L (86,13 %) and the highest was at a concentration of 0.7 mg/L with an inhibition value of 90.03%.
Keywords: Antioxidant, DPPH, Stylissa carteri, Mantehage
ABSTRAK
Spons ialah salah satu spesies dari filum porifera juga merupakan invertebrata laut yang hidup pada ekosistem terumbu karang. Spons merupakan biota laut multi sel yang fungsi jaringan dan organnya sangat sederhana. Spons Stylissa carteri dapat menghasilkan metabolit sekunder dari proses metabolisme dalam sel yang ada pada tubuhnya. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dari ekstrak etanol spons Stylissa carteri dari pulau Mantehage, Minahasa Utara. Spons Stylissa carteri diekstrak dengan metode maserasi menggunakan pelarut etanol. Pengujian aktivitas antioksidan dilakukan dengan metode DPPH (1,1-difenil-2- pikrilhidrazil) yang diukur dengan alat spektrofotometer UV-Vis. Hasil dari penelitian menunjukan bahwa ekstrak etanol spons Stylissa carteri memiliki aktivitas antioksidan disetiap konsentrasi, Pada konsentrasi 0,5 mg/L (77,7 %), konsentrasi 0,6 mg/L (86,13 %) dan yang tertinggi pada konsentrasi 0,7 mg/L dengan nilai inhibisi 90,03%.
Kata Kunci : Antioksidan, DPPH, Stylissa carteri, Mantehage
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)