Joice R.T.S.L Rimper; Silvana D Harikedua; Veibe Warouw

doi:10.35800/jasm.7.1.2019.25043

Authors

Joice R.T.S.L Rimper Marine Science Study Program, Faculty of Fisheries and Marine Science, Sam Ratulangi University, Manado, Indonesia
Silvana D Harikedua Fishery Product Technology Study Program, Faculty of Fisheries and Marine Science, Sam Ratulangi University, Manado, Indonesia
Veibe Warouw Marine Science Study Program, Faculty of Fisheries and Marine Science, Sam Ratulangi University, Manado, Indonesia

DOI:

https://doi.org/10.35800/jasm.7.1.2019.25043

Keywords:

rotifer, Brachionus rotundiformis, fish larvae, polymorphism, marine algae

Abstract

Title (Bahasa Indonesia): Variasi ukuran rotiferÂ Brachionus rotundiformisyang diberi pakan berbeda pada salinitas 40 ppt

Â

RotiferBrachionus rotundiformisis a group of zooplankton which is used by fish larvae for feeding to initiate their growth. This zooplankton is widely favored by marine fauna larvae because of its small size can fits well with various larval mouth; thus, it is easily preyed by larvae. This study aimed to determine the variation of rotiferÂ B. rotundiformismorphometry if cultured with different feed at 40 ppt. The use of 40 ppt salinity is expected to provide a variable morphometric size becauseÂ B. rotundiformishas a polymorphism property. Microalgae used as feed for rotiferÂ B. rotundiformiswereÂ Prochloronsp. andÂ Nanochloropsis oculata. Microalgae were cultured with Hirata medium. In the early stages,Â B. rotundiformiswas cultured at optimum temperature (28 ÂºC) and salinity 20 ppt, then it was cultured at salinity 40 ppt. Salinity adaptation was done by raising the salinity of the medium by 2 ppt every two days in a 10 ml reaction tube containing 10 individuals. After adaptation,Â B. rotundiformiswas transferred in a 1000 ml container with a density of 50 individuals. For the morphometric aspect, the total length, the length of the lorica, the width of the lorica and the anterior width were measured. The result showed the morphometric of rotiferB. rotundiformisfed with microalgaeÂ Prochloronsp. at 40 ppt salinity was smaller than that of the rotifer fed withÂ N. oculata. Based on that finding it can be concluded thatÂ B. rotundiformisÂ fed withÂ Prochloronsp. at a salinity of 40 ppt has the potential to be developed as feed for fish larvae. Further investigations on how to accelerate the cultivation of microalgaeÂ Prochloronsp. as feeding forÂ B. rotundiformisare needed.

RotiferBrachionus rotundiformismerupakan golongan zooplankton yang digunakan sebagai makanan bagi larva ikan. Zooplankton ini banyak disukai oleh larva fauna laut, karena ukurannya kecil yang cocok dengan berbagai bukaan mulut larva. Penelitian ini bertujuan untuk mengetahui variasi morfometri rotiferÂ B. rotundiformis, jika dikultur pada salinitas yang tinggi (40 ppt) dengan pemberian pakan berbeda. Penggunaan salinitas 40 ppt diharapkan bisa memberikan ukuran morfometrik yang bervariasi, karena rotifer jenis ini memiliki sifat polimorfisme. Alga mikro yang digunakan sebagai pakan adalahÂ Prochloronsp. DanÂ Nanochloropsis oculata.Alga mikro tersebut dikultur dalam media Hirata. Pada tahap awal,Â B. rotundiformisdikultur pada suhu optimum (28 ÂºC) dengan salinitas 20 ppt; kemudian, dikultur pada salinitas 40 ppt. Adaptasi salinitas dilakukan dengan menaikkan salinitas medium sebanyak 2 ppt setiap dua hari dalam tabung reaksi berukuran 10 ml, yang berisi 10 individu. Setelah diadaptasikan, rotifer dipindahkan ke wadah berukuran 1000 ml dengan kepadatan sebanyak 50 individu dan dikultur pada salinitas 40 ppt. Aspek morfometri berupa panjang total, panjang lorica, lebar lorica, dan lebar anterior diukur. Hasil penelitian menunjukkan, bahwa panjang total rotiferÂ B. rotundiformis, yang diberi pakanÂ Prochloronsp. berukuran lebih kecil dibandingkan dengan rotifer yang diberi pakanÂ N. oculata. Berdasarkan hasil penelitian dapat disimpulkan, bahwaÂ B. rotundiformisyang diberi pakanÂ Prochloronsp. pada salinitas 40 ppt memiliki potensi untuk dikembangkan sebagai bahan pakan bagi larva ikan. PenelitianÂ lebih lanjut tentang cara mempercepat budidaya microalgaeÂ Prochloronsp. sebagai makan untukÂ B. rotundiformisÂ diperlukan.

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