Cytotoxic Activity and Antiangiogenesis of Extract Fraction of Coccinia grandis (L.) Voigt) Base Towards T47D Breast Cancer Cells and Chorio Allantoic Membrane (CAM) of Chicken Embryo Induced by bFGF
Abstract
Aktivitas Sitotoksik dan Antiangiogenesis Ekstrak Fraksi Umbi Mentimun Papasan (Coccinia grandis (L.) Voigt) Terhadap Sel Kanker Payudara T47D Dan Chorio Allantoic Membrane (CAM) Embrio Ayam yang Diinduksi bFGF
Kata Kunci: Antiangiogenesis; Chorio Allantoic Membrane (CAM); mentimun papasan (Cocconia Grandis (L.) Voigt); Sitotoksik; T47D
Cytotoxic Activity and Antiangiogenesis of Extract Fraction of Coccinia grandis (L.) Voigt) Base Towards T47D Breast Cancer Cells and Chorio Allantoic Membrane (CAM) of Chicken Embryo Induced by bFGF
ABSTRACT
This study aims to identify the cytotoxic activity and antiangiogenesis of extract and fraction of (Coccinia Grandis (L.) Voigt) base towards T47D breast cancer cells and Chorio Allantoic Membrane (CAM) of chiken embryo induced by bFGF. The extract was extracted throught maceration method with ethanol 96% soluble. The ethanol extract was then fractionated by the liquid-liquid partition method. The fractions obtained were analyzed qualitatively by the thin layer chromatography (TLC) method. The TLC analysis shows that there are several classes of active compounds, namely alkaloids, flavonoids, tannins, saponons and terpenes. The ethanol extraction was then fractionated using the liquid-liquid extraction. The cytotoxic test was conducted using the T47D breast cancer cells and VERO cells with the MTT assay method which their absorbance were read on ELISA reader with the value of observation parameters IC50. The antiangiogenesis test was conducted using the Chorio Allantoic Membrane (CAM) method of chicken embryo induced with bFGF protein with the new blood vessels count as the observation parameters. The results showed that the extract, n-hexane fraction, ethyl acetate fraction, and water fraction had IC50 values of 500.167; 353.131; 109.975; and 303.990 µg/mL, respectively. Ethyl acetate fraction has the smallest IC50 value so it is continued to the antiangiogenesis test. Ethyl acetate fraction had an antiangiogenesis activity towards Chorio Allantoic Membrane (CAM) with the effective concentration 219,95 µg/mL marked by the angiogenesis inhibitors percentage of 56,78%.
Keywords: Antiangiogenesis; Chorio Allantoic Membrane (CAM); Coccinia grandis (L.) Voigt); cytotoxic; T47DFull Text:
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DOI: https://doi.org/10.35799/jis.20.1.2020.27032
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