PENGARUH APLIKASI CAHAYA TERHADAP SPERMATOZOA MENCIT JANTAN (MUS MUSCULUS L)
Abstract
Abstract: Light as a form of physical and psychological stress responses activated central and peripheral endocrine system in general interfere with the process of spermatogenesis especially the quantity and quality of the sperm of mice consisted of concentration, morphology and motility. The purpose of this study was to determine the effect of the application of the quality of light as well as the mechanism of spermatozoa of mice. Experimental studies with a completely randomized design done in November and December 2012 using 27 mice as a sample for 30 days, each consist of nine mice as a control, nine mice were given 4 lamp light exposure (12 volts) and 9 others mice were given a presentation by 8 light exposure (24 volt). The results of this study found that the percentage of motility in the first nine treated mice by 25.22% abnormal and in the second treatment 35.66% were abnormal; concentration of spermatozoa in the first treatment were 7.43X105 abnormal and the second treatment 6.45X105 were abnormal; morphology of spermatozoa in the first treatment were 57.88% abnormal and 67.22% abnormal in the second treatment. The conclusion of this study found that the provision of light affect the quality of spermatozoa of mice include motility, morphology, concentration; and the higher the light application, the lower the quality spermatozoa in mice will be.
Keywords: Light, Stress, Spermatozoa Quality of mice
Abstrak: Cahaya merupakan bentuk stress fisik dan psikologis mengaktifkan respon sentral dan perifer sistem endokrin yang secara umum mengganggu proses spermatogenesis khususnya terhadap kuantitas dan kualitas spermatozoa mencit terdiri dari konsentrasi, morfologi dan motilitas. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh aplikasi cahaya serta mekanismenya terhadap kualitas spermatozoa mencit. Penelitian eksperimental dengan rancangan acak lengkap di lakukan pada bulan November sampai Desember 2012 dengan menggunakan sampel sebanyak 27 ekor mencit selama 30 hari, masing-masing terdiri dari 9 ekor mencit sebagai kontrol, 9 mencit di beri pemaparan cahaya sebanyak 4 lampu (12 volt) dan 9 mencit lainnya di beri pemaparan cahaya sebanyak 8 lampu (24 volt). Hasil dari peneltian ini didapatkan persentase motilitas pada 9 mencit perlakuan pertama sebesar 25,22% abnormal dan pada perlakuan kedua 35,66% abnormal, konsentrasi spermatozoa pada perlakukan pertama 7,43x105 abnormal dan pada perlakuan kedua 6,45x105 abnormal, morfologi spermatozoa pada perlakukan pertama 57,88% abnormal dan pada perlakuan kedua 67,22% abnormal. Kesimpulan dari penelitian ini didapatkan bahwa pemberian cahaya lampu berpengaruh terhadap kualitas spermatozoa mencit dalam hal ini motilitas, morfologi, konsentrasi dan semakin aplikasi cahaya di naikkan maka kualitas spermatozoa mencit akan menurun.
Kata kunci: Cahaya, Stres, Kualitas Spermatozoa mencit.
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PDFDOI: https://doi.org/10.35790/ebm.v1i1.4575
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