Efek Suhu dan Waktu Simpan terhadap Kualitas Bagian Tengah Yellowfin Tuna Segar (Thunnus albacares)
Abstract
Decline quality of Tuna as a result of abuse temperature and long shelf life time. Histamine, microbial and sensory odours become one of the global problems that can cause health problems. Large number of bacteria dominated in viscera area rather than other fish parts. This study was conducted to determine the effect of storage temperature on histamine, microbial and sensory odours on middle of Yellowfin Tuna. This study used descriptive explorative method, sampel were periodically taken for analyses at intervals 48 hours (0ºC), 8 hours (10ºC) and 4 hours (25ºC). Yellowfin Tuna was rejected earlier by the sensory odours than TPC and histamine. During storage at 0ºC for 720 hours histamine levels still acceptable for consumption however TPC already exceeded the limit after 672 hours and rejected by sensory odours for 624 hours storage. During storage at temperature 10ºC did not reached the limit for 120 hours while TPC value already reached 2x106 cfu/g for 88 hours storage and rejected by sensory odours for 72 hours storage. During storage at temperature 25ºC histamine reached 67.3 ppm for 32 hours, ALT reached 5.5x105 cfu/g for 24 hours and rejected by sensory odour for 20 hours of storage. Histamine formation correlates with the growth of microbial counts and decrease of sensory odours value.
Suhu yang tinggi dalam waktu simpan lama berpengaruh terhadap penurunan kualitas ikan tuna. Histamin, mikroba dan bau busuk menjadi salah satu permasalahan global yang dapat menyebabkan masalah kesehatan. Bagian tengah ikan menjadi sumber bakteri paling tinggi dibandingkan bagian lainnya. Penelitian ini bertujuan untuk mengkaji perkembangan histamin, ALT dan sensori bau pada bagian tengah Tuna sirip kuning dalam penyimpanan suhu yang berbeda. Penelitian ini menggunakan metode deskriptif eksploratif, sample di analisis secara berkala pada suhu 0ºC (48 jam), 10ºC (8 jam) dan 25ºC (4 jam). Tuna sirip kuning mengalami pembusukan lebih awal berdasarkan parameter sensori bau, kemudian disusul ALT dan Histamin. Kandungan histamine pada suhu 0ºC masih layak untuk dikonsumsi setelah 720 jam, namun nilai ALT melebihi batas aman setelah 672 jam penyimpanan dan nilai sensori bau setelah 624 jam penyimpanan. Penyimpanan suhu 10ºC tidak menyebabkan peningkatan histamin melebihi batas limit setelah penyimpanan selama 120 jam, sedangkan nilai ALT mencapai 2x106 cfu/g setelah 88 jam dan nilai sensori bau menyatakan ikan busuk setelah penyimpanan selama 72 jam. Penyimpanan suhu 25ºC histamin mencapai 67,3 ppm setelah penyimpanan 32 jam dan nilai ALT 5,5x105 cfu/g pada penyimpanan ke 24 jam serta ikan dinyatakan busuk berdasarkan sensori bau setelah 20 jam penyimpanan. Peningkatan histamin berkorelasi dengan pertumbuhan jumlah mikroba dan penurunan nilai sensori bau.
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[BSN] Badan Standardisasi Nasional. (2015). Cara Uji Mikrobiologi-Bagian 3: Penentuan Angka Lempeng Total (ALT) pada produk perikanan. SNI 2332.3:2015. Jakarta: BSN.
[BSN] Badan Standardisasi Nasional. (2006). Tuna beku-Bagian 1: Spesifikasi. SNI 01-2710.1:2006. Jakarta: BSN.
Biji, K. B., Ravishankar, C. N., Venkateswarlu, R., Mohan, C. O., & Gopal, T. K. S. (2016). Biogenic amines in seafood: a review. Journal of Food Science and Technology, 53 (5), 2210–2218. https://doi.org/10.1007/s13197-016-2224-x
Cinquina, A. L., Longo, F., Calì, A., De Santis, L., Baccelliere, R., & Cozzani, R. (2004). Validation and comparison of analytical methods for the determination of histamine in tuna fish samples. Journal of Chromatography A, 1032 (2004), 79–85. https://doi.org/10.1016/j.chroma.2003.11.033
DiGregorio, R. (2012). Tuna Grading and Evaluation : The complete Tuna Buyer’s Handbook (M. Zicarellu (ed.)). Urner Barry.
Du, W. X., Lin, C. M., Phu, A. T., Cornell, J. A., Marshall, M. R., & Wei, C. I. (2002). Development of biogenic amines in yellowfin tuna (Thunnus albacares): Effect of storage and correlation with decarboxylase-positive bacterial flora. Journal of Food Science. https://doi.org/10.1111/j.1365-2621.2002.tb11400.x
FAO, & WHO. (2013). Public Health Risks of Histamine and other Biogenic Amines from Fish and Fishery Products. In Meeting report.
[FDA] U.S. Food and Drug Administration. (2019). Fish and Fishery Products Hazards and Controls Guidance - Fourth Edition. In Fish and Fishery Products Hazard and Control Guidance Fourth Edition. https://doi.org/10.1039/9781847558398-00136
Guizani, N., Al-Busaidy, M. A., Al-Belushi, I. M., Mothershaw, A., & Rahman, M. S. (2005). The effect of storage temperature on histamine production and the freshness of yellowfin tuna (Thunnus albacares). Food Research International, 38 (2), 215–222. https://doi.org/10.1016/j.foodres.2004.09.011
Hansamali, L. H. D., Ranatunga, R. R. M. K. P., Buddhinie, P. K. C., & Ashoka, P. (2020). Determination of shelf life of Yellowfin tuna (Thunnus albacares) with regard to microbial count, histamine level and flesh colour. Sri Lanka Journal of Aquatic Sciences, 25 (1), 1–8. https://doi.org/10.4038/sljas.v25i1.7571
Jinadasa, B. K. K. K., Galhena, C. K., & Liyanage, N. P. P. (2015). Histamine formation and the freshness of yellowfin tuna (Thunnus albacares) stored at different temperatures. Cogent Food & Agriculture. https://doi.org/10.1080/23311932.2015.1028735
Lee, Y. C., Tseng, P. H., Hwang, C. C., Kung, H. F., Huang, Y. L., Lin, C. Saint, Wei, C. I., & Tsai, Y. H. (2019). Effect of Vacuum Packaging on Histamine Production in Japanese Spanish Mackerel (Scomberomorus niphonius) Stored at Various Temperatures. Journal of Food Protection, 82 (11), 1931–1937. https://doi.org/10.4315/0362-028X.JFP-19-143
Lehane, L., & Olley, J. (2000). Histamine fish poisoning revisited. In International Journal of Food Microbiology. https://doi.org/10.1016/S0168-1605(00)00296-8
López-Sabater, E. I., Rodríguez-Jerez, J. J., Hernádez-Herrero, M., Roig-Sagués, A. X., & Mora-Ventura, M. T. (1996). Sensory quality and histamine formation during controlled decomposition of tuna (Thunnus thynnus). Journal of Food Protection, 59 (2), 167–174. https://doi.org/10.4315/0362-028X-59.2.167
Mahusain, N. A. S., Bayoi, F., Karim, N. U., Zainol, M. K., & Danish-Daniel, M. (2016). Determination of histamine levels in long-tail tuna (Thunnus tonggol) stored at different temperature. https://doi.org/10.7287/peerj.preprints.2360
Mangunwardoyo, W., Sophia, R. A., & Heruwati, E. S. (2007). Seleksi dan Pengujian Aktivitas Enzim L-Histidine Decarboxylase dari Bakteri Pembentuk Histamin. MAKARA of Science Series, 11 (2), 104–109. https://doi.org/10.7454/mss.v11i2.292
Nurjanah, Asadatun, A., & Kustiariyah. (2011). Pengetahuandan Karakteristik Bahan Baku Hasil Perairan. IPB Press.
Silbande, A., Adenet, S., Smith-Ravin, J., Joffraud, J. J., Rochefort, K., & Leroi, F. (2016). Quality assessment of ice-stored tropical yellowfin tuna (Thunnus albacares) and influence of vacuum and modified atmosphere packaging. Food Microbiology, 60 (2016), 62–72. https://doi.org/10.1016/j.fm.2016.06.016
Starling, E., & Diver, G. (2005). The Australian tuna handling manual: a practical guide for industry. Seafood Services Australia.
Tahmouzi, S., Ghasemlou, M., Aliabadi, F. S., Shahraz, F., Hosseini, H., & Khaksar, R. (2013). Histamine formation and bacteriological quality in skipjack tuna (katsuwonus pelamis): Effect of defrosting temperature. Journal of Food Processing and Preservation, 37 (2013), 306–313. https://doi.org/10.1111/j.1745-4549.2011.00650.x
Tao, Z., Nakano, T., Yamaguchi, T., & Sato, M. (2002). Production and diffusion of histamine in the muscle of scombroid fishes. Fisheries Science. https://doi.org/10.2331/fishsci.68.sup2_1394
Widiastuti, I., Putro, S., Fardiaz, D., Trilaksani, W., & Inaoka, T. (2013). Changes in freshness of steak and loin tuna (Thunnus albacares) during 15 day-chilled storage. Journal of Fisheries and Aquatic Science, 8 (2), 367–377. https://doi.org/10.3923/jfas.2013.367.377
Wodi, S. I. M., Cahyono, E., & Mamontho, N. (2016). Mutu Ikan Pindang Selar (Selaroides Sp.) pada Berbagai Konsentrasi Ekstrak Daun Kemangi. Jurnal Ilmiah Tindalung, 2(1), 36–41.
Wodi, S. I. M., Trilaksani, W., & Nurilmala, M. (2018). Histamin dan Identifikasi Bakteri Pembentuk Histamin pada Tuna Mata Besar(Thunnus obesus). Jurnal Teknologi Perikanan Dan Kelautan Vol, 9(2), 185–192.
DOI: https://doi.org/10.35800/mthp.8.3.2020.29537
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