PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN PAKU SISIK NAGA (DRYMOGLOSSUM PILOSELLOIDES L.PRESL) TERHADAP PEROKSIDASI LIPID HATI PADA TIKUS JANTAN GALUR WISTAR YANG DIINDUKSI CCL4

Abstract

The aims of this research was to determine the effect of ethanol extract paku sisik naga
leaves against lipid peroxidation in male wistar liver induced by CCl4. Method of this
research used laboratory experiment using 9 white male wistar were divided into 3 treatment
groups. All groups were given treatment during ten days. The first group (negative control)
was given distilled water ad libitum, the second group was given ethanol extract of paku sisik
naga leaves 97.02 mg/kg body weight per oral and the third group (positive control) was
given Curcuma 45 mg/kg body weight per oral. On the tenth day, after two hours of
treatment all groups were given CCl4 1mL/kg body weight intraperitonial, eighteen hours
after that rats in all groups were dissected and taken his liver. Then rat liver analyzed by
TBARS method (Thiobarbiturate Acid Reactive Subtance) to measure the levels of MDA
(Malondialdehyde) which is an indicator of lipid peroxidation in the rat liver. Data were
analyzed by SPSS ver.20, significant difference between treatments was tested by one-way
ANOVA. The results showed a significant difference with 95% confidence level (α = 0.05)
with a mean value of MDA 3,828 μM negative control group, group of ethanol extract of
paku sisik naga leaf was 2,666 μM and 2,211 μM for positive control group. Ethanol extract
of paku sisik naga leaves with dose 97.02 mg/kg body weight can prevent the process of lipid
peroxidation in rat liver.
Keywords: Drymoglossum piloselloides L. Presl, CCl4, TBARS, lipid peroxidation