ANALISIS ZAT PEWARNA RHODAMIN B PADA KERUPUK YANG BEREDAR DI KOTA MANADO

Abstract

ABSTRACT
Rhodamine B dye in the form of a crystalline powder green or reddish purple, odorless, and
dissolves easily in bright red solution berfluoresan as textile dyes. Rhodamine B is still no
food products were found to contain rhodamine B dye such as crackers, sauces, ice and other
pastries. The purpose of this study is to investigate and determine the levels of rhodamine B
on crackers circulating in the city of Manado. Sampling sites crackers are 4 market in the city
of Manado is Tuminting Market, Market Paal 2, Market Market Bersehati 45 and 45.
Samples with ammonia to soak interesting rhodamine B dye using wool yarn, followed by
identification using thin layer chromatography (TLC) and then in detection with UV light 254
nm and 366 nm. The reading levels of rhodamine B using UV-Vis spectrophotometry. The
results showed that of the ten samples tested with three times obtained one positive sample
containing rhodamine B with an average concentration value of rhodamine B in a sample of
45 markets in the sword of 0.28 mg/ml. Based on these results, some crackers circulating in
Manado City Market unsafe consumed.
Keywords: Rhodamine B, Crackers, spectrophotometry and Thin Layer Chromatography