ANALISIS KEBERADAAN RHODAMIN B PADA IKAN CAKALANG FUFU YANG BEREDAR DI PASARAN KOTA MANADO

Abstract

Rhodamin B is a synthetic dye that was banned for use in foods and is certified as a hazardous
material according to Minister of Health of Indonesian Republic No. 722/Menkes/Per/IX/1988
because it can cause liver, kidney, and spleen damage, and followed by anatomical changes in the
form of organ enlargement. Rhodamin B dye is often used for a food and beverage products. The
study was conducted to identify Rhodamin B on cakalang fufu in the market of Manado city.
Cakalang fufu samples were taken from four markets in the city of Manado, namely : the Paal 2
market, Bahu market, Karombasan market and Bersehati 45 Market. Samples were extracted by two
methods, the method of extraction followed by purification and extraction method using wool yarn
and absorption followed by identification using thin layer chromatography (TLC) and detected with
UV light 254 nm and 366 nm. Rhodamin B content is read using a UV-Vis spectrophotometry. The
result showed that out of eight samples examined with two tests, there are four samples containing
Rhodamin B with higher levels of the average value of Rhodamin B in samples, that is from the
merchant two of Bahu Market is 0,054 ug/ml, at merchant two Karombasan market is 0.184 ug/ml,
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merchant one at Bersehati 45 Market is 0.103 ug/ml and merchant two at Bersehati 45 is 0.514 ug/ml.
Based on these results, some of cakalang fufu circulating in Manado City Markets are not safe for
consumption.
Keywords: Rhodamin B, Cakalang fufu, TLC and UV-Vis spectrophotometry