ISOLASI DAN IDENTIFIKASI SENYAWA FLAVANOID PADA DAUN ADAM HAWA (Rhoe discolor)

Abstract

One of the plants can be used as a drug crop is Adam Hawa (Rhoe discolor) are thought to contain
flavanoid compounds, it is seen from the purple on the leaf. This study aims to determine the presence
or absence of flavonoids and other types of flavanoid compounds are contained in the leaf Adam
Hawa (Rhoe discolor). Isolation of flavonoids is done by maceration. Flavanoid compound
identification is done by preparative Thin Layer Chromatography (KLTP) using the eluent n-butanol:
acetic acid: water (BAA) with a ratio of 4:1:5. Identification of flavanoid compounds made with UVVis
spectrophotometer. The results showed the leaf extract of Adam Hawa (Rhoe discolor) contains
flavanoid compounds. Seen from the results of preparative thin layer chromatography to yield three
spots with Rf value of 0.09; 0.36, and 0.71. Based on the analysis of UV-Vis spectrophotometer, three
isolates with Rf value of 0.71 has a maximum wavelength of 275 nm.
Keyword : Adam Eve leaf (Rhoe discolor), Flavanoid, Preparative Thin Layer Chromatography
(KLTP), UV-Vis spectrophotometer.