UJI DAYA HAMBAT EKSTRAK ETANOL DAUN KEMBANG SEPATU (Hibiscus rosa-sinensis L) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli

Abstract

The prevalence of diarhea in Indonesia is considered high and one cause is Escherichia coli. The aim of this research was to determine anti-bacterial inhibition, effective concentration, and the effect of increasing concentration of ethanol extract of Hibiscus rosa-sinensis to the growth of E. coli. Extract of H. rosa-sinensis was obtained by masseration using 95% ethanol as solvent. Bacterial growth inhibition employed Kirby-Bauer Method using agar diffusion through wells. Measurement of bacterial inhibition was analysed using one way anova and continued with Least Significantly Difference. Anova showed inhibition by 5%, 10%, 20%, 40%, 80% of extracts. Effective concentrations occured on 20% and 40%. The increasing of extract concentration showed correlation with the increasing of inhibition zone diameters.

Key words : Hibiscus rosa-sinensis leaf, ethanol extract, inhibition, E. coli