Sunscreen Test of Methanol Extract, N-Hexane, Ethyl Acetate and Methanol-Water Fraction of Artocarpus lanceifolius Roxb. Leaves Based on In Vitro Method

Authors

  • Eva Marliana Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman, Samarinda, Indonesia
  • Novia Rahmawati Isyahro Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman, Samarinda, Indonesia
  • Nanang Tri Widodo Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman, Samarinda, Indonesia

DOI:

https://doi.org/10.35799/jis.v23i2.51543

Keywords:

Artocarpus lanceifolius, In Vitro, Sunscreen

Abstract

Sunscreen test of  keledang (Artocarpus lanceifolius Roxb.) leaves based on in vitro method was carried out. This research aimed to investigate the property of sunscreen from methanol extract, n-hexane, ethyl acetate and methanol-water fraction of keledang leaves. The methods used include the in vitro methods by using spectrophotometer UV-Vis. Based on results of phytochemical test, it was known that  methanol extract contains flavonoids, phenolics, steroids/triterpenoids. n-Hexane fraction contains steroids/triterpenoids and quinones. Ethyl acetate fraction contains flavonoids, phenolics, steroids/triterpenoids and quinones. Methanol-water fraction contains flavonoids and phenolics. Methanol extract, n-hexane, ethyl acetate and methanol-water fraction also showed sunscreen activity based on the SPF value at a concentration of 25; 50; 100; 150; 200 and 250 mg/L. Crude methanol extract has SPF value respectively were 4.44; 7.36; 15.15; 22.47; 31.25 and 37.31 (minimum-ultra). N-Hexane fraction has SPF value respectively were 1.37; 2.24; 5.07; 7.51; 10.01 and 12.74 (minimum-maximum). Ethyl acetate fraction has SPF value respectively were 3.321; 7.20; 13.03; 19.54; 25.97 and 30.59 (minimum-ultra). Methanol-water fraction has SPF value respectively were 5.24; 10.87; 21.48; 30.89; 36.01 and 38.52 (medium-ultra). The SPF value increases with increasing concentration because the higher the concentration, the more active sunscreen compounds contained in the extract and fraction. In keledang leaves, methanol-water fraction has the highest suncreen activity folowed by methanol extract, ethyl acetate fraction and n-hexane fraction. Keledang leaves have a potency as  sunscreen agent.

Keywords: Artocarpus lanceifolius; in vitro; sunscreen

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Published

2023-10-31

How to Cite

Marliana, E., Isyahro, N. R., & Widodo, N. T. (2023). Sunscreen Test of Methanol Extract, N-Hexane, Ethyl Acetate and Methanol-Water Fraction of Artocarpus lanceifolius Roxb. Leaves Based on In Vitro Method. Jurnal Ilmiah Sains, 23(2), 158–167. https://doi.org/10.35799/jis.v23i2.51543

Issue

Volume 23 Nomor 2, Oktober 2023

Section

Articles

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Copyright (c) 2023 Eva Marliana, Novia Rahmawati Isyahro, Nanang Tri Widodo

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License