VIABILITAS PUPA PARASITOID Diadegma semiclausum Hellen (Hymenoptera : Ichneumonidae) BERDASARKAN LAMA PENYIMPANAN DI LEMARI PENDINGIN

Abstract

ABSTRACT This study aims to determine the viability of the D. semiclausum parasitoid pupae on P. xylostella larvae stored in the refrigerator. The results of this study are expected to provide information about the viability of D. semiclausum parasitoid pupae which are stored in refrigerators so that they can assist farmers in carrying out biological control. The study was conducted in the area of farmers' cabbage growing in the Kakaskasen II Village, Tomohon City and the Entomology and Plant Pest Laboratory, Faculty of Agriculture, Sam Ratulangi University. The research lasted for four months from July to October 2019. The study used a survey method on farmers' cabbage flower plants. The location of the collection of P. xylostella and D. semiclausum pupae was carried out by purporsive sampling, which is collecting pupae where there are flower cabbage plants in Kakaskasen II Village. In the initial stage, pupae collection is carried out with the aim of propagation of P. xylostella pests. Furthermore, if the larvae of P. xylostella have entered the initial instar, then the collection of D. semiclausum parasitoid pupae with the aim of being released in a cage to paralyze the P. xylostella larvae. The results showed that the parasitoid pupa can survive in the refrigerator until the 34th day with viabiltas of 10.00%. Furthermore, on the 36th day until the 42nd day, the D. semiclausum parasitoid pupae were no longer found that had successfully survived into adult insects. When related to the duration of storage of the D. semiclausum parasitoid pupae in the refrigerator with pupa viability, the best pupae storage time is on the 22nd day with the percentage of parasitoid pupa viability of 75.00%.

Keywords : Viability, P. xylostella, D. semiclausum, Tomohon