Uji Daya Hambat Perasan Daging Buah Alpukat (Persea americana Mill.) terhadap Pertumbuhan Bakteri Staphylococcus aureus
DOI:
https://doi.org/10.35790/eg.6.1.2018.19653Abstract
Abstract: To date, Staphylococcus aureus resistance to some antibiotics is still increasing inter alia the methisillin-resistant Staphylococcus aureus (MRSA). Therefore, it is necessary to find other alternative materials that could overcome this bacteria. Avocado (Persea americana Mill.) is a medicinal plant that contains antibacterial compounds such as saponins, glutathiones, flavonoids, and tannins in its fruit flesh. This study was aimed to obtain the inhibitory effect of avocado flesh on the growth of S. aureus. This was a true experimental study with a post test only control group design. We used Kirby-Bauer modification with paper disks. The positive control was ciprofloxacin and the negative control was aquadest. The avocado flesh was refined by using a juicer and then was filtered. Staphylococcus aureus bacteria were obtained from pure bacteria stock at Microbiology Laboratory of Pharmacy Study Program at Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The result showed that the avocado juice had an inhibitory effect on the growth of S. aureus. The mean diameter of inhibitory zones formed was 15.55 mm which was classified as a strong inhibitory effect. Conclusion: The avocado flesh had a strong inhibitory effect on the growth of Staphylococcus aureus.
Keywords: avocado flesh (Persea americana Mill.), S. aureus, inhibitory effect
Â
Abstrak: Resistensi bakteri Staphylococcus aureus terhadap beberapa jenis antibiotik sudah cukup tinggi. Sebagai contoh ialah methicillin-resistant Staphylococcus aureus (MRSA). Oleh karena itu perlu dicari bahan alternatif lain yang dapat mengatasi berkembang biaknya bakteri ini. Buah alpukat (Persea americana Mill.) merupakan salah satu tanaman obat yang dikenal berkhasiat sebagai antibakteri karena terdapat kandungan senyawa antibakteri pada daging buah seperti saponin, glutatin, flavonoid, dan tanin. Penelitian ini bertujuan untuk mengetahui daya hambat perasan daging buah alpukat (Persea americana Mill.) terhadap pertumbuhan bakteri S. aureus. Jenis penelitian ini ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu modifikasi Kirby-Bauer dengan kertas saring. Kontrol positif menggunakan antibakteri ciprofloxacin dan kontrol negatif menggunakan akuades. Daging buah alpukat dihaluskan dengan menggunakan juicer dan disaring. Bakteri S. aureus diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian menunjukkan bahwa perasan daging buah alpukat memilki daya hambat terhadap pertumbuhan bakteri S. aureus. Diameter rerata zona hambat yang terbentuk yaitu 15,55 mm dan digolongkan sebagai zona hambat yang kuat. Simpulan: Perasan daging buah alpukat (Persea americana Mill.) memiliki daya hambat kuat terhadap pertumbuhan bakteri S. aureus.
Kata kunci: daging buah alpukat (Persea americana Mill.), S. aureus, daya hambat
Downloads
How to Cite
Issue
Section
License
COPYRIGHT
Authors who publish with this journal agree to the following terms:
Authors hold their copyright and grant this journal the privilege of first publication, with the work simultaneously licensed under a Creative Commons Attribution License that permits others to impart the work with an acknowledgment of the work's origin and initial publication by this journal.
Authors can enter into separate or additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (for example, post it to an institutional repository or publish it in a book), with an acknowledgment of its underlying publication in this journal.
Authors are permitted and encouraged to post their work online (for example, in institutional repositories or on their website) as it can lead to productive exchanges, as well as earlier and greater citation of the published work (See The Effect of Open Access).
