Efektivitas Ekstrak Daun Kamboja (Plumeria rubra L.) terhadap Bakteri Enterococcus faecalis
DOI:
https://doi.org/10.35790/366gw581Keywords:
antibakteri; daun kamboja; Enterococcus faecalis; perawatan saluran akarAbstract
Abstract: Enterococcus faecalis exhibits a high prevalence in root canal treatment (RCT) failure. Although 2,5% NaOCl is effective in eliminating Enterococcus faecalis, it can cause apical tissue irritation and has an unpleasant odor. Herbal ingredients offer antibacterial properties with lower toxicity levels. Frangipani leaves (Plumeria rubra L.) contain antibacterial compounds such as flavonoids, phenols, and alkaloids. This study aimed to determine the effectiveness of frangipani leaf extract (Plumeria rubra L.) in inhibiting the growth of Enterococcus faecalis. This was a true experimental in vitro laboratory design using the well diffusion method. Frangipani leaf extract was obtained through a 96% ethanol maceration process. Treatment groups consisted of various concentrations of frangipani leaf extract (20%, 40%, 60%, 80%), a positive control (2.5% NaOCl) and a negative control (aquadest). Enterococcus faecalis cultures were obtained from the FMIPA Laboratory at Universitas Sam Ratulangi. The results indicated based on the mean diameter of the inhibition zones, the inhibitory strength from the highest to the lowest was: the positive control, 80%, 60%, 40%, 20% of frangipani leaf extract, and negative control. The Kruskal–Wallis test showed a p-value <0,05 indicating significant differences in the mean inhibition zone diameters across all groups. In conclusion, Frangipani leaf extract (Plumeria rubra L.) is effective in inhibiting the growth of Enterococcus faecalis with the 80% of extract being the most effective and classified as having strong inhibitory activity.
Keywords: antibacterial effect; frangipani leaf; Enterococcus faecalis; root canal treatment
Abstrak: Bakteri Enterococcus faecalis memiliki prevalensi tinggi pada kasus kegagalan perawatan saluran akar (PSA). NaOCl 2,5% efektif mengeliminasi Enterococcus faecalis, namun dapat menimbulkan iritasi jaringan apikal dan berbau tidak sedap. Daun kamboja (Plumeria rubra L.) memiliki senyawa bersifat antibakteri seperti flavonoid, fenol dan alkaloid. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak daun kamboja (Plumeria rubra L.) terhadap pertumbuhan bakteri Enterococcus faecalis. Jenis penelitian true eksperimental laboratorium secara in vitro melalui metode difusi sumuran. Ekstrak daun kamboja diperoleh melalui metode maserasi etanol 96%. Kelompok perlakuan ekstrak daun kamboja dengan konsentrasi berbeda (20%, 40%, 60%, 80%), kontrol positif (NaOCl 2,5%), dan kontrol negatif (akuades). Bakteri Enterococcus faecalis yang digunakan berasal dari Laboratorium FMIPA Universitas Sam Ratulangi. Hasil penelitian menunjukkan bahwa urutan daya hambat berdasarkan rerata diameter zona hambat dari yang terbesar hingga terkecil ialah kontrol positif, konsentrasi ekstrak 80%, 60%, 40%, 20%, dan kontrol negatif. Uji Kruskal-Wallis menunjukkan nilai p<0,05 yang berarti terdapat perbedaan rerata diameter zona hambat pada setiap kelompok perlakuan. Simpulan penelitian ini ialah ekstrak daun kamboja (Plumeria rubra L.) efektif menghambat pertumbuhan bakteri Enterococcus faecalis dengan konsentrasi paling efektif yaitu 80% dan tergolong daya hambat kategori kuat.
Kata kunci: antibakteri; daun kamboja; Enterococcus faecalis; perawatan saluran akar
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