Antibacterial substances of sponges, Agelas tubulata and Phyllospongia sp., from Manado Bay, against the growth of several bacterial strains

Authors

  • Nani I.J Undap Program Studi Magister Ilmu Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi
  • Deiske A Sumilat Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi
  • Robert Bara Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi

DOI:

https://doi.org/10.35800/jasm.5.1.2017.24253

Keywords:

Agelas tubulata, Phyllospongia sp., extract of sponges, antibacteriall

Abstract

Title (Bahasa Indonesia): Senyawa antibakteri spons, Agelas tubulata dan Phyllospongia sp., dari Perairan Teluk Manado, terhadap pertumbuhan beberapa strain bakteri

 

Agelas tubulataand Phyllospongiasp. are marine spongethat contain active compoundsthat have potential to beantibacterial, anticancer and antifungal which have not been used. This study was aimed to examine the sponge extracts which inhibitbacterial growth, i.e. Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Escherichia coli, S. saprophyticus andtoanalyze the active fraction of the antibacterial component using bio-autographic method. Agelas tubulataand Phyllospongiasp. were collected from Manado Bayand method used in the study was Agar Diffusion Method. Results showed that A. tubulataand Phyllospongiasp. extract had antibacterial activity against all 5 bacteria tested. A. tubulataextract had inhibition zone withbroader spectrum than that of Phyllospongiasp. extract, while Phyllospongiasp. has specifity forP. mirabilis.


Agelas tubulata danPhyllospongia sp. merupakan spesies spons laut yang mengandung senyawa aktif yang berpotensi antibakteri, antikanker dan antijamur yang belum banyak dimanfaatkan. Penelitian ini bertujuan untuk menguji ekstrak spons laut yang menghambat pertumbuhan 5 bakteri yaitu Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Eschericia colidan Staphylococcus saprophyticus dan menganalisis fraksi aktif antibakteri melalui teknik bioautografi. Spesies spons laut A. tubulata dan Phyllospongiasp. diambil dari Perairan Pantai Malalayang Manado. Metode yang digunakan yaitu metode difusi agar. Hasil dari penelitian ini, yaitu ekstrak A. tubulatadan Phyllospongiasp. mempunyai aktivitas terhadap 5 bakteri uji. EkstrakA. tubulatamemiliki daya hambat dengan spektrum yang lebih luas dibandingkan ekstrak Phyllospongiasp.sedangkanekstrak Phyllospongia sp.memiliki spesifitas terhadap bakteri P. mirabilis.

References

BARA, R., ALY, A. H., PRETSCH, A., WRAY, V., WANG, B., PROKSCH, P., DEBBAB, A. (2013). "Antibiotically Active Metabolites from Talaromyces wortmannii, an endophyte of Aloe vera." The Journal of Antibiotics 10.1038/ja.2013.28.

BARA, R. A., ZERFAß, I., LAI, D., LIN, W., DEBBAB, A., BRÖTZ-OESTERELT, H., PROKSCH, P. (2013). "New Natural Product from Botryosphaeria australis, an Endophyte from Mangrove Avicennia marina." Squalen Bulletin of Marine & Fisheries Postharvest & Biotechnology 8(3): 139-145.

BLUNT, J.W., COPP, B.R., KEYZERS, R.A., MUNRO, M.H.G. and PRINSEP, M.R. 2012. Marine natural products. Nat. Prod. Rep. 29: 144–222, and previous reports in this series.

BLUNT, J.W.; COPP, B.R.; MUNRO, M.H.G.; NORTHCOTE, P.T.; PRINSEP, M.R., 2004. Marine Natural products. Nat Prod Rep. 21,1-49.

CHOMA, I. 2005. The use of thin layer chromatography with direct bioautography for antymicrobial analysis. Journal of Chromatography A.

DAVIS, W.W. and STOUT, T. R. 1971. Disc Plate Method of Microbiological Assay.

DENNING, D. 2006. Branches on the tree of Life: Sponges. Retrieved Februari 23, 2016, from http://ebiomedia.com/prod/BOsponges.html.

MANASA, G. 2014. Structural Elucidation of Terpenoids by Spectroscopic Techniques. Journal of Chemical and Pharmaceutical Sciences. ISSN: 0974-2115.

ORTEZ, J. 2005. Disk Diffusion Testing in Manual of Antimicrobial Susceptibility testing. Marie B. Coyle (Coord. Ed). American society for Microbiology.

PINHEIRO, P and GONCALO J. 2012. Structural Analysis of Flavonoids and Related Compounds-A Review of Spectroscopic Applications. Technical University of Lisbon. Portugal.

POOLE, K. 2007. Efflux Pumps as Antimicrobial Resistance Mechanisms. Annals of Medicine, 39(3):162-176.

POSANGI, J., Bara, R. 2014. Analisis Aktivitas dari Jamur Endofit yang terdapat dalam Tumbuhan Bakau Avicennia marina di Tasik Ria Minahasa. Jurnal Pesisir Dan Laut Tropis , 1(1)30-38.

ROMPAS, R. 2011. Farmakognosi Laut (Sumber Baru ekonomi Kelautan). Jakarta: Dewan Kelautan Indonesia.

SILVERSTEIN, R.M., WEBSTER, F.X., & KIEMBLE, D.J. (2005). Spectrometric Identification of Organic Compounds. 7 ed. USA: John Wiley and Sons.

TALEB-CONTINI, S. H., SALVADOR, M. J., WATANABE, E., ITO, I. Y. OLIVEIRA, D. C. R. D. (2003). "Antimicrobial Activity of Flavonoids and Steroids Isolated from Two Chromolaena Species". Brazilian Journal of Pharmaceutical Sciences 39(4).

TYUKAVKINA, N.A., POGODAEVA N.N. and LUTSKI V. I (1970). Ultraviolet absorption of Flavonoids. Irkutstak Institute of Organic Chemistry, Siberian Division. Khimiya Prirodynkh Soedinenli, Vol. 6, No.1. pp 24-27.

WALKER D., ROBERT, JR. and HAWKINS J. E. 1952. The Ultraviolet Absorption Spectra of Some Terpene Hydrocarbons. Journal of the American Chemical Society (ACS) Publications.

Downloads

Published

2017-04-30

Issue

Vol. 5 No. 1 (2017): APRIL

Section

Articles

License

COPYRIGHT & LICENSES:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under an Attribution 4.0 International (CC BY 4.0) that allows others to share — copy and redistribute the material in any medium or format — and adapt — remix, transform, and build upon the material for any purpose, even commercially — with an acknowledgment of the work's authorship and initial publication in this journal.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.