Fahruddin Fahruddin; Slamet Santosa; Sareda .

doi:10.35800/jasm.8.1.2020.29572

Authors

Fahruddin Fahruddin Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Univeristas Hasanuddin, Makassar 90245, Indonesia
Slamet Santosa Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Univeristas Hasanuddin, Makassar 90245, Indonesia
Sareda . Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Univeristas Hasanuddin, Makassar 90245, Indonesia

DOI:

https://doi.org/10.35800/jasm.8.1.2020.29572

Keywords:

lead, bacterial isolates, resistancy, Makassar

Abstract

Marine water which has been polluted by heavy metals such as lead (Pb) from port activities can affect marine life, however several microorganisms are able to adapt in polluted waters due to their resistant capabilities. The research aimed to obtain isolates bacterial which are resistant to Pb and to test their resistant ability. Bacterial isolation was obtained from sediment and seawater taken in Paotere Port, Makassar. The isolation of the resistant bacteria was done using nutrient agar media which contained 10 ppm of Pb. The growth of the bacteria was initially observed macroscopically and microscopically to determine type of the colony. Resistant test for each type of isolate was done by inoculating them on nutrient broth media which contained different concentration of Pb: 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, and a control. The test resulted in eight different isolates, five isolates from sediment samples and three from seawater samples. The results showed that each isolate has different degree of resistant toward Pb. Isolate IA1 has the higher resistant ability, and it was followed by isolate IS5.

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Perairan laut tercemar logam berat timbal (Pb), yang bersumber dari aktifitas pelabuhan, dapat mengganggu kehidupan biota laut, namun sejumlah mikrorganisme mampu beradaptasi karena memiliki kemampuan resistan.Â Penelitian ini bertujuan untuk memperoleh isolat bakteri yang resistan terhadap Pb dan menguji kemampuan resistansinya. Isolasi bakteri diperoleh dari sampel sedimen dan air laut dari Pelabuhan Paotere, Makassar.Untuk isolasi bakteri resistan menggunakan media nutrient agar yang megandung 10 ppm Pb. Bakteri yang tumbuh diamati secara makropskopis dan mikroskopis sebagai pendekatan awal untuk mengetahui jenis isolat. Uji resistansi dilakukan untuk setiap jenis isolat, yang diinokulasikan pada media nutrient broth pada konsentrasi Pb sebesar 5 ppm, 10 ppm, 15 ppm. 20 ppm, 25 ppm dan 0 ppm sebagai kontrol.Â Hasilnya, diperoleh delapan jenis isolat yang berbeda, yaitu lima jenis isolat dari sampel sedimen dan tiga dari sampel air laut. Dari hasil uji memperlihatkan, bahwa setiap isolat memiliki kemampuan resistansi berbeda-beda terhadap Pb. Isolat IA1 kemampuan resistansinya lebih tinggi, kemudian isolat IS5.

References

ADACHI, A., OKIAYU, M., NISHIKAWA, A. and KOBAYASHI, T. (1998) Metal levels in rain water from Kobe City in Japan. Bulletin of Environmental Contamination and Toxicology 60(6), pp. 892-897.

CHELLAIAH, E.R. (2018) Cadmium (heavy metals) bioremediation by Pseudomonas aeruginosa: a minireview. Applied Water Science, 8 (154), pp. 1-10.

FAHRUDDIN and ASADI, A. (2015) Use of organic materials wetland for improving the capacity of sulfate reduction bacteria (SRB) in reducing sulfate in acid mine water (AMW). Asian Journal of Microbiology, Biotechnology and Environ-mental Sciences17 (21), pp. 321-324.

FAHRUDDIN, F., EVA, J. and ZARASWATI, D. (2019) Antifouling potential of Thalassia hemprichii extract against growth of bioï¬lm-forming bacteria. ScienceAsia, 45, pp.21-27.

FAHRUDDIN, F., NURHAEDAR, SLAMET, S. and SRI, W. (2019) Uji kemampuan tumbuh isolat bakteri dari air dan sedimen sungai Tallo terhadap logam timbal (Pb). Jurnal Ilmu Alam dan Lingkungan 10(2), pp. 58-64.

FISKANITA, F., HAMZAH, B. and SUPRIADI, S. (2015) Analisis logam timbal (Pb) dan besi (Fe) dalam air laut di Pelabuhan Desa Paranggi Kecamatan Ampibabo. Jurnal Akademika Kimia, 4 (4), pp. 175-180.

JORGENSEN, I., ZHANG, Y., KRANTZ, B.A. and MIAO, E.A. (2016) Pyroptosis triggers pore-induced intracellular traps (PITs) that capture bacteria and lead to their clearance by efferocy-tosis. Journal of Experimental Medicine, 213 (10), pp. 2113-2128.

MCGENITY, T.J., FOLLWEL, B.D., MCKEW, B.A. and SANNI, G.O. (2012) Marine crude-oil biodegradation: a central role for interspecies interactions. Aquatic Biosystems, 8 (10), pp. 1-19.

NAIK, M.M., SHAMIM, K. and DUBEY, S.K. (2012) Biological characterization of lead-resistant bacteria to explore role of bacterial me-tallothionein in lead resistance. Current Science, 103 (4), pp. 426-429.

RAHIM, I.R. and ALI, S.H.(2014) Studi pengelolaan sampah Pelabuhan Soekarno-Hatta, Makassar. Makassar: Universitas Hasanuddin.

RACHMAWATY, F.J., DEWA, A.C., BUNGA, N., TITIS, N. and ENDRAWATI, T.B. (2009) Manfaat Sirih Merah (Piper crocatum) sebagai agen anti bakterial terhadap bakteri Gram positif dan bakteri Gram negatif. Jurnal Kedokteran dan Kesehatan Indonesia 1(1), pp.1-7.

ROCHYATUN, E., KAISUPY, M.T. and ROZAK, A. (2006) Distribusi logam berat dalam air dan sedimen di perairan muara Sungai Isadane. Makara Sains, 10 (1), pp. 35-40.

TANJUNG, R.E., FAHRUDDIN, F. and FARID, S. (2019) Absorption of heavy metal lead (Pb) by water hyacinth (Eichhornia crassipes) and Its influence to total dissolved solids of groundwater in phytoremediation. Indonesia Chimica Acta 13 (1), pp. 10-15.

THOMPSON, G.A. and WATLING, R.J. (1987) Bioaccumulation potential of heterotrophic bacteria for lead, selenium, and arsenic. Bulletin of Environmental Contamination and Toxicology, 38 (6), pp. 1049-1054.

THOMPSON, J.A.J. and CRERAR, J.A. (1980) Methylation of lead in marine sediments. Marine Pollution Bulletin, 11 (9), pp.251-253.

TSUNEDA, A. and CURRAH, R.S. (2004) Knufia endospora, a new clematiaceous hyphomycete from trembling aspen. Canada: Tottori Mycolo-gical Institute.

WULANDARI, S., DEWI, N.F. and SUWONDO. (2005) Identifikasi bakteri pengikat timbal (Pb) pada sedimen di perairan Sungai Siak. Jurnal Biogenesis, 1 (2), pp. 62-65.

YAHYA, M. (2013) Rekayasa lingkungan Pelabuh-an Pendaratan Ikan (PPI) di Pelabuhan Paotere Makassar. Makassar: Jurnal Lab. Perencanaan dan Perancangan Lingkungan, Fakultas Teknik Universitas Hasanuddin.