Dias Asthisa; Desy M.H. Mantiri; Deiske A. Sumilat; Rizal M. Rompas; Annita Ch. Sinjal; Rose O.S.E. Mantiri

doi:10.35800/jasm.v9i2.35199

Authors

Dias Asthisa Program Studi Magister Ilmu Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi, Jl. Kampus Unsrat Bahu, Manado 95115 Sulawesi Utara, Indonesia
Desy M.H. Mantiri Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi, Jl. Kampus Unsrat Bahu, Manado 95115, Sulawesi Utara, Indonesia
Deiske A. Sumilat Program Studi Magister Ilmu Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi, Jl. Kampus Unsrat Bahu, Manado 95115 Sulawesi Utara, Indonesia
Rizal M. Rompas Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi, Jl. Kampus Unsrat Bahu, Manado 95115, Sulawesi Utara, Indonesia
Annita Ch. Sinjal Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi, Jl. Kampus Unsrat Bahu, Manado 95115, Sulawesi Utara, Indonesia
Rose O.S.E. Mantiri Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi, Jl. Kampus Unsrat Bahu, Manado 95115, Sulawesi Utara, Indonesia

DOI:

https://doi.org/10.35800/jasm.v9i2.35199

Keywords:

algae, Kappaphycus alvarezi, bioactive compound, LC-HRMS, Minahasa

Abstract

Algae Kappaphycus alvarezii is one of the leading commodities from fisheries and marine sectors in Indonesia. This algae species has many benefits in the fields of marine biology, chemistry, pharmacy, and pharmacology, as well as an excellent nutritional content for health. It is cultivated in the coastal waters of Minahasan Peninsula, especially in Buku Village, Belang District, Southeast Minahasa Regency. This study was carried out to analyze bioactive compound in the algae. Samples of 2-weeks old were taken directly from the cultivation area. Screening test was done qualitatively while an advanced test was done using LC-HRMS method. The results showed 4 bioactive compounds detected in the sample, they are alkaloids, tannins/phenolics, saponins, and flavonoids. From the advanced test, it was found 10 major compounds in the sample, i.e. (2-ethylhexyl) phthalate, phthalic acid, D-(+)-proline, maraniol, stigmatellin Y, Pentyl isopentyl phthalate, methyl thiofentanyl, phenylethanolamine, acetyl hexamethyl tetralin, and fucoxanthin.

Indonesian title: Senyawa bioaktif pada alga Kappaphycus alvarezii dari perairan Belang, Kabupaten Minahasa Tenggara

References

AGARWAL, A. (2016) Duality of anti-nutritional factors in pulses. Journal of Nutritional Disorders & Therapy, 6 (1), pp. 1-2.

AL-BAYATI, R.I., AL-AMIERY, A.A.H. and AL-MAJEDY, Y.K. (2010) Design, synthesis and bioassay of novel coumarins. African Journal of Pure and Applied Chemistry, 4 (6), pp. 74-86.

ARIFIN, B. and IBRAHIM, S. (2018) Struktur, bioaktivitas dan antioksidan flavonoid. Jurnal Zarah, 6 (1), pp. 21-29.

BAI, Y., LIU, Z., BI, Y., WANG, X., JIN, Y., SUN, L., WANG, H., ZHANG, C. and XU, S. (2012) Preparation of polyclonal antibodies, and development of a direct competitive enzime-linked immunosorbent assay to detect residues of phenylthanolamine A in urine sampels. Journal of Agricultural and Food Chemistry, 60 (46), pp. 11618-11624.

BOLD, H.C. and WYNNE, M.J. (1985) Introduction to the Algae. Pretice, Inc. England.

BOOPATHI, S., VASHISTH, R., MANOHARAN, P., KANDASAMY, R. and SIVAKUMAR, N. (2017) Stigmatellin Yâ€“an anti-biofilm compound from Bacillus subtilis BR4 possibly interferes in PQSâ€“PqsR mediated quorum sensing system in Pseudomonas aeruginosa. Bioorganic & Medicinal Chemistry Letters, 27 (10), pp. 2113â€“2118.

Dâ€™ORAZIO, N., GEMELLO, E., GAMMONE, M.A., de GIROLAMO, M., FICONERI, C. and RICCIONI, G. (2012). Fucozantin: a treasure from the sea. Marine Drugs, 10 (3), pp. 604-616.

DEWI, S.T.R. and WAHYUNI, S. (2018) Uji efek anti inflamasi rebusan daun jamblang (Syzygium cumini) pada mencit (Mus musculus). Media Farmasi, 14 (1), pp. 53- 59.

DHORA, A. (2017) Ekstraksi tanin pada daun ketapang (Terminalia catappa L.) dengan variasi pelarut, konsentrasi pelarut dan lama waktu ekstraksi. Jurnal Agroindustri Halal, 3 (2), pp. 142-151.

DIGGLE, S.P. and Whiteley, M. (2020) Microbe profile: Pseudomonas aeruginosa: opportunistic pathogen and lab rat. Microbiology (Reading), 166 (1), pp. 30-33.

FAHRUNI, F., HANDAYANI, R. and NOVAR-YATIIN, S. (2018) Potensi tumbuhan kelakai (Stenochlaena palustris (Burm.F.) Bedd.) asal Kalimantan Tengah sebagai afrodisiaka. Jurnal Surya Medika, 3 (2), 144-153.

HAMIM, H., ASHRI, K., MIFTAHUDIN and TRIADIATI (2008) Analisi status air, prolin dan aktivitas enzim antioksidan beberapa kedelai toleran dan peka kekeringan serta kedelai liar. Agrivita, 30 (3), 201-210.

HANA, C.M., SUNYOTO and ROHMAT, N. (2018) Penetapan kadar tanin dari kulit buah pisang raja masak (Musa Paradisiaca L.) secara spektrofotometri UV-Vis. Motorik, 13 (26), pp. 28-39.

HARBORNE, J.B. (1987) Metode Fitokimia: Penuntun cara modern menganalisis tumbuhan. Terbitan Kedua. Bandung: ITB Bandung.

JASWIR, I., NOVIENDI, D., SALLEH, H.M., TAHER, M. and MIYASHITA, K. (2011) Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines. African Journal of Biotechnology, 10 (81), pp. 18855-18862.

KATSIKANTAMI, I., SIFAKIS, S., TZATZARA-KIS, M.N., VAKONAKI, E., KALANTZI, O-I., TSATSAKIS, A.M. and RIZOS, A.K. (2016) A global assessment of phthalates burden and related links to health effects. Environment International, 97, pp. 212-236.

KHOTIMAH, K.K., Darius, D.D. and SASMITO, B.B. (2013) Uji aktivitas senyawa aktif alga cokelat (Sargassum fillipendulla) sebagai antioksidan pada minyak ikan lemuru (Sardinella longiceps). THPi Student Journal, I (1), pp. 10-20.

MALANGNGI, L., SANGI, M. and PAENDONG, J. 2012. Penentuan kandungan tanin dan uji aktivitas antioksidan ekstrak biji buah alpukat (Persea americana Mill.). Jurnal MIPA Unsrat Online, 1 (1), pp. 5-10.

MITA, S.R. and HUSNI, P. (2017). Pemberian pemahaman mengenai penggunaan obat analgesik secara rasional pada masyrakat di Arjasari Kabupaten Bandung. Dharmakarya: Jurnal Aplikasi Ipteks untuk Masyarakat, 6 (3), pp. 193-195.

NCUBE, N.S., AFOLAYAN, A.J. and OKOH, A.I. (2008) Assesment technique of antimicrobial properties of natural compound of plant origin: current methods and future trends. African Journal of Biotechnology, 7 (12), pp. 1797-1806

NINGRUM, R., ELLY, P. and SUKARSONO (2016) Identifikasi senyawa alkaloid dari batang karamunting (Rhodomyrtus tomentosa) sebagai bahan ajar biologi untuk SMA kelas X. Jurnal Pendidikan Biologi Indonesia, 2 (3), pp. 231-236.

NOVIENDRI, D., JASWIR, I., SALLEH, H.M., TAHER, M., MIYASHITA, K. and RAMLI, N. (2011). Fucoxanthin extraction and fatty acid analysis of Sargassum binderi and S. duplicatum. Journal of Medicinal Plants Research, 5 (11), pp. 2405-2412.

PECK, A.M. and HORNBUCKLE, K.V. (2004) Synthetic musk fragrances in Lake Michigan. Environmental Science and Technology, 38 (2), pp. 367-372.

PENG J., YUAN, J-P., WU, C-F. and WANG, J-H. (2011) Fucoxanthin, a marine carotenoid present in brown seaweeds and diatoms: metabolism and bioactivities relevant to human health. Marine Drugs, 9 (10), pp. 1806-1828.

PRATIWI, R.D. (2016) Uji Kualitatif fitokimia daun Ruta angustifolia. Faktor Exacta, 9 (3), pp. 200-206.

PURNOBASUKI, H. (2004) Potensi mangrove sebagai tanaman obat. Biota, IX (2), pp. 125-126.

SAAD, M.M-E. (2014) The most recent hadard of phthalate that threaten food safety and human health. International Journals of Medical Science and Clinical Inventions, 1 (10), pp. 527-535.

SALEH, A.A.G.A., ASNANI, A. and SUWARJO-YOWIRAYATNO, S. (2019) Uji fitokimia dan aktivitas antioksidan cendol yang diformulasi dari rumput laut (Kappaphycus alvarezii) dan tepung sagu (Metroxylon sagus Rottb.). Jurnal Fish Protech, 2 (1), pp. 38-45.

SARIRA, N.H. and PONG-MASAK, P.R. (2019) Seaweed selection to supply superior seeds for cultivation. Jurnal Perikanan Universitas Gadjah Mada, 20 (2), pp. 79-85.

TRONO, G.C. (1997) Field Guide and Atlas of the Seaweed. National Book Store. Philippines: Publisher Metro Manila.

TSOCHATZIS, E.D., TZIMOU-TSITOURIDOU, R. and GIKA, H.G. (2017) Analytical methodologies for the assessment of phthalate exposure in humans. Critical Reviews in Analytical Chemistry, 47 (4), pp. 279-297.

WANG, Q., JIN, J., DAI, N., HAN, N., HAN, J. and BAO, B. 2016. Anti-inflammatory effects, nuclear magnetic resonance identification, and high-performance liquid chromatography isolation of the total flavonoids from Artemisia frigida. Journal of Food and Drug Analysis, 24 (2), pp. 385-391.

YANUARTONO, PURNAMANINGSIH, H., NUR-URROZI, A. and INDARJULIANTO, S. (2017) Saponin: dampak terhadap ternak. Jurnal Peternakan Sriwijaya, 6 (2), pp. 79-90.