Pengaruh Kinetin Pada Pertumbuhan Kecambah Brokoli Hibrida (Brassica oleracea Var. Green Magic) Dan Kandungan Sulforafan Pada Kultur In Vitro

Authors

  • Monica Pamela Doodoh Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado
  • Wenny Tilaar Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado
  • Sesilia Wanget Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado

DOI:

https://doi.org/10.35791/agrsosek.v18i3.45023

Keywords:

broccoli; tissue culture; sulforaphane; kinetin

Abstract

This study aims to observe the growth in the sprouting phase and to observe the sulforaphane content in the growth stages of broccoli sprouts treated with kinetin on MS media. The research was conducted at the Laboratory of Biotechnology, Faculty of Agriculture, Sam Ratulangi University and the Laboratory of Department of Chemical Engineering, State of Malang. The study used a Completely Randomized Design (CRD) with 4 treatments and 9 replications, nam ely without kinetin, 0.5 ppm kinetin, 1 ppm kinetin, 1.5 ppm kinetin. The variables observed were the time of sprout formation, sprout height, sprout weight, sulforaphane content. The results of this study showed that the sulforaphane content was not affected by the height and weight of the sprouts but the kinetin concentration, the higher the concentration given, the higher the sulforaphane content.

Author Biographies

Monica Pamela Doodoh, Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado

Mahasiswa Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado

Wenny Tilaar, Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado

Dosen Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado

Sesilia Wanget , Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado

Dosen Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado

References

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Sutopo, L. 1985. Teknologi Benih. Fakultas Pertanian Universitas Brawijaya Malang. Rajawali. Jakarta.

Tilaar, W. 2017. Mikropropagasi Brokoli (Brassica Oleracea L. Var. Italica Plenck) & Biosintesis Sulforafan. Pascasarjana Unsrat.

Tilaar, W., S. Ashari., B. Yanuwiadi., J. Polii-Mandang., dan F.H. Tomasowa. 2012. Shoot Induction from Broccoli Excplant Hypocotyls and Biosynthesis of Sulforaphane. International Journal of Basic & Applied Sciences IJBAS-IJENS, 12(06), 44-48.

Tilaar, W., J. Mandang., dan A. Pinaria. 2018. Analisis Kandungan Sulforafan Pada Beberapa Fase Pertumbuhan Dari Beberapa Jenis Brassica Sp Secara In Vivo. Laporan Riset Terapan Unggulan Universitas Sam Ratulangi.

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Published

2022-09-28

How to Cite

Doodoh, M. P., Tilaar, W., & Wanget , S. (2022). Pengaruh Kinetin Pada Pertumbuhan Kecambah Brokoli Hibrida (Brassica oleracea Var. Green Magic) Dan Kandungan Sulforafan Pada Kultur In Vitro. AGRI-SOSIOEKONOMI, 18(3), 835–842. https://doi.org/10.35791/agrsosek.v18i3.45023

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Articles