Phytochemical Screening and Antioxidant Activity Assay of Macroalgae Eucheuma sp. Ethanol Extract

Maya E.W.  Moningka; Christi D. Mambo; Finny  Warouw

doi:10.35800/h4yv7a67

Authors

Maya E.W. Moningka Universitas Sam Ratulangi
Christi D. Mambo
Finny Warouw Universitas Sam Ratulangi

DOI:

https://doi.org/10.35800/h4yv7a67

Keywords:

phytochemical screening, Eucheuma sp., antioxidants; DPPH

Abstract

Indonesia is a maritime nation possessing vast coastal and marine territories. It is also renowned for the richness and diversity of biological natural resources found within its marine waters. Macroalgae are a component of marine flora, and their characteristics are significantly influenced by the quality of the marine environment. The chemical compounds produced by macroalgae exhibit anti-inflammatory, antimicrobial, antiviral, antimutagenic, antioxidant, and anticancer activities. Research Methodology: This experimental study aimed to identify the specialized metabolites of Eucheuma sp. through qualitative and quantitative phytochemical screening and to assess antioxidant activity using the DPPH radical scavenging method. Data analysis was conducted descriptively based on the obtained results. The findings indicate that the metabolites in Eucheuma sp. include flavonoids, tannins, saponins, and phenolics, with the highest concentration observed in phenolic compounds 2.9698 ppm. The Eucheuma sp. macroalgae extract demonstrated moderate antioxidant activity, with an IC₅₀ value of 121 ppm.

Keywords: phytochemical screening, Eucheuma sp., antioxidants, DPPH

Abstrak

Indonesia merupakan negara bahari yang memiliki wilayah pesisir serta lautan yang sangat luas. Indonesia juga terkenal karena kekayaan dan keanekaragaman sumber daya alam hayati yang terkandung di perairan laut Indonesia. Makroalga merupakan salah satu dari flora yang ada diperairan laut dimana makroalga sangat dipengaruhi oleh kualitas lingkungan perairan laut. Senyawa kimia yang dihasilkan oleh makroalga memiliki aktivitas sebagai antiinflamasi, antimikroba, antivirus, antimutagen, antioksidan dan antikanker. Metodologi Penelitian: Penelitian eksperimen ini dilakukan untuk mengidentifikasi kandungan metabolit terspesialisasi dari Eucheuma sp. dengan uji skrining fitokimia baik secara kualitatif maupun secara kuantitatif serta melakukan uji aktivitas antioksidan menggunakan metode Radikal DPPH. Analisis data dilakukan secara deskriptif berdasarkan hasil yang didapat. Hasil penelitian, menunjukkan senyawa metabolit dari Eucheuma Sp terdiri dari flavonoid, tanin, saponin dan fenolik dengan kandungan senyawa fenolik paling tinggi yaitu 2,9698 ppm Ekstrak makroalga Eucheuma Sp memiliki kandungan antioksidan yang tergolong aktivitas sedang dengan nilai IC₅₀ sebesar 121 ppm.

Kata kunci: skrining fitokimia, Eucheuma sp., antioksidan, DPPH

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