EFEKTIVITAS ANTIBAKTERI EKSTRAK BIJI BENGKUANG (Pachyrrhizus erosus) TERHADAP PERTUMBUHAN Streptococcus mutans SECARA IN VITRO

Authors

  • Ian Handry Supari

DOI:

https://doi.org/10.35799/pha.5.2016.12935

Abstract

EFEKTIVITAS ANTIBAKTERI EKSTRAK BIJI BENGKUANG (Pachyrrhizus erosus) TERHADAP PERTUMBUHAN Streptococcus mutans SECARA IN VITRO

Ian Handry Supari1), Michael A. Leman1), Kustina Zuliari1)

1)Program Studi Pendidikan Dokter Gigi, Fakultas Kedokteran, UNSRAT, Manado, 95115

 

ABSTRACT

Dental caries is an oral health problems that often occurs. Streptococcus mutans is a bacteria main causes of dental caries. Research using natural materials has been carried out. One of the useful plants is the Yam. Yam seed (Pachyrrhizus erosus) contains flavonoids, saponins, tannins, quinones, alkaloids, triterpenoids. This study aims to determine the effectiveness of antibacterial Yam seeds extract on the growth of Streptococcus mutans in vitro. This research was a laboratory experimental with the study design post test only control group design. Testing methods used in this study was modified Kirby-Bauer method using the sinks. MHA media provided as many as five a petri dish with 15 wells, five wells filled with a solution of yam seeds extract, five wells filled amoxicilin and five other wells filled with ethanol 96 %. Observation were made after twenty-four hours the incubation.The result showed that yam seeds extract has antibacterial effect to inhibit the growth of streptococcus mutans bacteria, but the inhibition zone smaller than amoxicillin.

Keywords: Yam seeds (Pachyrrhizus erosus), Streptococcus mutans, inhibition zone

 

ABSTRAK

Karies gigi merupakan masalah kesehatan gigi dan mulut yang sering terjadi. Streptococcus mutants merupakan bakteri utama penyebab karies gigi. Penelitian dengan menggunakan bahan alam telah banyak dilakukan. Salah satu tumbuhan yang bermanfaat ialah bengkuang. Biji bengkuang (Pachyrrhizus erosus) mengandung senyawa antibakteri antara lain flavonoid, saponin, tanin, kuonin, alkaloid, dan triterpenoid. Tujuan penelitian ini adalah untuk mengetahui efektivitas ekstrak biji bengkuang terhadap pertumbuhan Streptococcus mutans secara in vitro. Penelitian ini merupakan penelitian eksperimental laboratoris dengan rancangan penelitian post test only control group design. Metode pengujian yang digunakan dalam penelitian ini ialah metode modifikasi Kirby-Bauer dengan menggunakan sumuran. Media MHA disediakan sebanyak lima cawan petri dengan 15 buah sumur, lima sumur diisi dengan larutan ekstrak biji bengkuang, lima sumur diisi amoksisilin dan lima sumur lainnya diisi dengan etanol 96%. Pengamatan dilakukan setelah 24 jam masa inkubasi. Hasil penelitian ini menunjukan bahwa ekstrak biji bengkuang memiliki efek antibakteri untuk menghambat pertumbuhan bakteri Streptococcus mutans, tetapi zona hambatnya lebih kecil dibandingkan dengan antibiotik amoksisilin.

Kata kunci: biji bengkuang (Pachyrrhizus erosus), Streptococcus mutans, zona hambat

 

Downloads

Published

2016-08-02

How to Cite

Supari, I. H. (2016). EFEKTIVITAS ANTIBAKTERI EKSTRAK BIJI BENGKUANG (Pachyrrhizus erosus) TERHADAP PERTUMBUHAN Streptococcus mutans SECARA IN VITRO. PHARMACON, 5(3). https://doi.org/10.35799/pha.5.2016.12935

Issue

Vol. 5 No. 3 (2016): Pharmacon

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)