PENGARUH EKSTRAK KASAR YANG MENGANDUNG ENZIM PEROKSIDASE DARI SAWI HIJAU (Brassica juncea) TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK TONGKOL JAGUNG (Zea mays L.)
DOI:
https://doi.org/10.35799/pha.5.2016.12948Abstract
PENGARUH EKSTRAK KASAR YANG MENGANDUNG ENZIM PEROKSIDASE DARI SAWI HIJAU (Brassica juncea) TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK TONGKOL JAGUNG Â Â Â Â Â Â Â Â Â Â Â (Zea mays L.)
Wowor Patricia Walanda1), Edi Suryanto1), Jemmy Abidjulu1)
1)Program Studi Farmasi Fakultas MIPA UNSRAT Manado, 95115
ABSTRACT
The objective of this research was to study the effect of peroxidase enzymes in the greens mustard of the antioxidant activity extract corn cobs. Corn cobs extracted using reflux method for 2 hours. Peroxidase enzyme extraction of green mustard made in 4 comparison for further mixing with the corn cobs extract. The best results from the mixing, are added to the (NH4)2SO4. Determination of phytochemical content is done by calculating the total phenolic and flavonoid. Tests using the antioxidants counteract free radical DPPH and hydroxyl radical scavengers TBA at the Wistar rat liver homogenates. The Result snow that the extract corn cob with the addition of a peroxidase enzyme extract green mustard increased total phenolic and flavonoid content. Similarly, the antioxidant activity with DPPH and free radical hydroxyl scavengers Wistar rat liver homogenates, had an increase in free-radical scavengers. Conclusion of the research is the addition of peroxidase enzyme extract contained in greens mustard provide increased content of total phenolics and flavonoids, also increases the antioxidant activity of the corn cob extract.
Keywords : corn cobs, green mustard, antioxidant, peroxidase enzymes.
ABSTRAK
Tujuan penelitian ini adalah untuk mempelajari pengaruh enzim peroksidase dari sawi hijau (Brassica juncea) terhadap aktivitas antioksidan dari ekstrak tongkol jagung (Zea mays L.). Tongkol jagung diekstraksi menggunakan metode refluks selama 2 jam. Ekstraksi Enzim Peroksidase dari Sawi Hijau dibuat dalam 4 perbandingan untuk selanjutnya di lakukan pencampuran dengan ekstrak tongkol jagung. Hasil terbaik dari pencampuran, ditambahkan dengan (NH4)2SO4. Penentuan kandungan fitokimia dilakukan dengan menghitung total fenolik dan flavonoid. Pengujian antioksidan menggunakan penangkal radikal bebas DPPH dan penangkal radikal hidroksil TBA pada homogenat hati tikus wistar. Ekstrak tongkol dengan penambahan ekstrak enzim peroksidase dari Sawi hijau mengalami peningkatan kandungan total fenolik dan flavonoid. Sama halnya dengan aktivitas penangkal radikal bebas DPPH dengan penangkal radikal hidroksil homogenat hati tikus wistar, memiliki peningkatan penangkal radikal bebas. Hasil penelitian ini menyimpulkan bahwa penambahan ekstrak enzim peroksidase yang terkandung dalam sawi hijau memberikan peningkatan kandungan total fenolik dan flavonoid, juga meningkatkan aktivitas antioksidan pada ekstrak tongkol jagung.
Kata kunci :Â tongkol jagung, sawi hijau, antioksidan, enzim peroksidase.
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