ANALISIS FINGERPRINT DAUN GEDI HIJAU (Abelmoschus manihot L) UNTUK MEMPREDIKSI AKTIVITAS ANTIOKSIDAN MENGGUNAKAN KOMBINASI SPEKTROSKOPI IR DENGAN PARTIAL LEAST SQUAREREGRESSION

Authors

  • Marino N. Warongan

DOI:

https://doi.org/10.35799/pha.6.2017.17731

Abstract

ANALISIS FINGERPRINT DAUN GEDI HIJAU (Abelmoschus manihot L) UNTUK MEMPREDIKSI AKTIVITAS ANTIOKSIDAN MENGGUNAKAN

KOMBINASI SPEKTROSKOPI IR DENGAN PARTIAL

LEAST SQUAREREGRESSION

 

Marino Novri Warongan1), Sri Sudewi1), Adithya Yudistira1)

1)Program Studi Farmasi FMIPA UNSRAT Manado, 95115

 

 

ABSTRACT

 

The study aims to determine the antioxidant activity, infrared spectra and correlation between spectral data and antioxidant activity of green gedi leaves. Method of determining prediction of antioxidant activity through fingerprint analysis using combination of IR spectroscopy and Chemometric Partial Least Square Regression (PLSR). DPPH method is used to prevent free radical and free-radical yield of free radical or concentration 150 ppm Bitung A sample 91.392 ± 1.125, Bitung B sample 88.493 ± 1.515 dan Bitung C sample 91.663 ± 1.184, Manado A sample 89.464 ± 1.642, Manado B sample 90.096 ± 3.510, and Manado C sample 89.477 ± 0.850,  and in the Kotamobagu sample A 86.678 ± 3.703, Kotamobagu sample B 82.963 ± 2.991 and Kotamobagu sample C 83.163 ± 6.354. Gedi leaves from the city of Bitung, Manado, and Kotamobagu has a relatively similar spectrum. This combination involves the FTIR spectrum as the “x†variabel and the result data of the DPPH method as the “y†variabel. The prediction model can be used because it has error value ( standar error calibration (SEC = 0.0615), standard error of prediction (SEP = 0.0763)) and calibration value of 0.4181, and r validation of 0.2917.

 

Keywords: Antioxidant, Green Gedi Leaves, FTIR Spectrophotometry, UV-VIS Spectrophotometry, Chemometric .

 

 

ABSTRAK

 

Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan, spektrum infra merah dan korelasi antara data spektrum dan aktivitas antioksidan daun gedi hijau. Metode penentuan prediksi aktivitas antioksidan melaui analisis fingerprint menggunakan kombinasi Spektroskopi IR dan Kemometrik Partial Least Square Regression (PLSR). Metode DPPH digunakan untuk menangkal radikal bebas dan hasil persen penangkal radikal bebas pada konsentrasi 150 ppm Sampel Bitung A 91.392 ± 1.125, Sampel Bitung B 88.493 ± 1.515 dan Sampel Bitung C 91.663 ± 1.184, Sampel Manado A 89.464 ± 1.642, Sampel Manado B 90.096 ± 3.510, dan Sampel Manado C 89.477 ± 0.850, dan terakir pada Sampel Kotamobagu A 86.678 ± 3.703, Sampel Kotamobagu B 82.963 ± 2.991 dan Sampel Kotamobagu C 83.163 ± 6.354.Daun Gedi dari  kota Bitung, Manado dan Kotamobagu ini memiliki spektrum yang relatif sama. Kombinasi ini melibatkan dari spektrum FTIR sebagai variabelx dan data hasil analisis metode DPPH sebagai variabely. Model prediksi dapat digunakan karena memiliki nilai kesalahan (standar error calibration (SEC = 0.0615), standard error of prediction (SEP = 0.0763)) dan nilai r kalibrasi 0.4181, serta r validasi 0.2917.

 

Kata Kunci : Antioksidan, Daun Gedi Hijau, Spektrofotometri FTIR, Spektrofotometri UV-VIS,  Kemometrik.

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Published

2017-10-10

How to Cite

Warongan, M. N. (2017). ANALISIS FINGERPRINT DAUN GEDI HIJAU (Abelmoschus manihot L) UNTUK MEMPREDIKSI AKTIVITAS ANTIOKSIDAN MENGGUNAKAN KOMBINASI SPEKTROSKOPI IR DENGAN PARTIAL LEAST SQUAREREGRESSION. PHARMACON, 6(4). https://doi.org/10.35799/pha.6.2017.17731

Issue

Vol. 6 No. 4 (2017): Pharmacon

Section

Articles

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