EFEK PEMBERIAN ALPHA LIPOIC ACID PADA ENDOTEL TIKUS PUTIH YANG DIINDUKSI LIPOPOLISAKARIDA

Authors

  • Olvie Datu
  • Fransisco P Sumalong

DOI:

https://doi.org/10.35799/pha.9.2020.27418

Abstract

ABSTRACT

 

Endothelial dysfunction play a major role in the pathogenesis of vascular diseases, especially atherosclerosis. Endothelial dysfunction causes endothelial cells are activated and resulting homeostasis changes in endothelial cell. Rats were induced by LPS 0.5 mg/kg and treated with ALA at doses of 30 mg/kg, 60 mg/kg and 120 mg/kg BW for 2 weeks which given 1 hour before the LPS administration. This study show that LPS causes morphological changes in rat aorta based on histochemical staining show that endothelial cell are irregular, not homogeneous and increase permeability but in group that received show that ALA can improve the morphological of endothelial cell by reduces ROS, regenerates of exogenous and endogenous antioxidants. Administration of ALA and LPS reduce the rats body weight but not significant compared to the control group. Therefore can be concluded that administration of ALA can prevent endothelial dysfunction.

 

Keywords: Endothel dysfunction, alpha lipoic acid, lipopolysaccharide

 

 

ABSTRAK

 

Disfungsi endotel berperan penting pada pathogenesis penyakit vascular, khususnya aterosklerosis.  Disfungsi endotel menyebabkan sel endotel teraktivasi dan terjadi perubahan homeostatis pada sel endotel. Tikus diinduksi dengan LPS 0.5 mg/Kg BB dan selanjutnya di terapi dengan ALA pada dosis 30mg/Kg BB, 60mg/Kg BB, dan 120mg/Kg BB selama 2 minggu 1 jam sebelum pemerian LPS. Penelitian ini menujukan bahwa LPS dapat menyebabkan perubahan morfologi pada sel nedotel berdasakan pada hasil pewarnaan jaringan yang menunjukan sel endotel yang tersusun tidak beraturan, tidak homogeny dan terjadi peningkatan permeabilitas tapi pada kelompok perlakuan yang mendapatkan terapi ALA menunjukan ALA dapat memperbaiki morfologi sel endotel dengan mengurangi ROS, meningkatkan antioksidan eksogen dan endogen. Pemberian ALA dan LPS tidak menyebabkan perubahan berat badan tikus yang signifikan di bandingkan dengan kelompok control. Sehingga dapat disimpulkan pemberian ALA dapat mencegah disfungsi endotel.

 

Kata Kunci: disfungsi endotel, histokimia, alpha lipoic acid, lipopolisakarida

Downloads

Published

2020-02-28

How to Cite

Datu, O., & Sumalong, F. P. (2020). EFEK PEMBERIAN ALPHA LIPOIC ACID PADA ENDOTEL TIKUS PUTIH YANG DIINDUKSI LIPOPOLISAKARIDA. PHARMACON, 9(1), 125–130. https://doi.org/10.35799/pha.9.2020.27418

Issue

Vol. 9 No. 1 (2020): PHARMACON

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)