IDENTIFIKASI MOLEKULER BAKTERI DALAM FESES KUCING (Felis domestica) YANG DITUMBUHKAN PADA DE MANN ROGOSA SHARPE AGAR (MRSA)

Authors

  • Pingkan Stela Mende Jurusan Biologi, Fakultas MIPA, Universitas Sam Ratulangi
  • Johanis Pelealu Jurusan Biologi, Fakultas MIPA, Universitas Sam Ratulangi
  • Beivy Kolondam Jurusan Biologi, Fakultas MIPA, Universitas Sam Ratulangi

DOI:

https://doi.org/10.35799/pha.8.2019.29239

Abstract

ABSTRACT

Bacteria has many important role in the digestive tract of animals. Beneficial bacteria in the digestive tract are thought to be able to inhibit the growth of pathogenic bacteria, while pathogenic bacteria can cause diseases and infections. This research aimed to grow bacteria living in cat feces and to identify it with molecular method. This study used moleculer identification based on 16S rRNA gene as marker. There were three isolate if bacteria taken from the culture. Two isolates were identified as Enterococcus faecalis (with 99% and 100% in similarity compared with GenBank database). One isolate was identified as Kurthia gibsonii (100% in similarity). Keywords: Bacteria, cat feces, MRS Agar, gen 16s rRNA

 

ABSTRAK

Bakteri memiliki peran penting dalam saluran pencernaan hewan. Bakteri yang menguntungkan dalam saluran pencernaan dianggap mampu menghambat pertumbuhan bakteri patogen, sedangkan bakteri yang merugikan dalam saluran pencernaan hewan dianggap mampu menyebabkan penyakit dan infeksi. Penelitian ini bertujuan untuk menumbuhkan bakteri-bakteri yang ada dalam feses kucing dan mengidentifikasikannya dengan metode molekuler. Penelitian ini menggunakan identifikasi molekuler berdasarkan gen 16S rRNA sebagai penanda. Hasil penelitian ini mendapatkan tiga isolat bakteri. Dua diantaranya teridentifikasi sebagai Enterococcus faecalis (kemiripan 99% dan 100% dengan yang ada di GenBank). Satu isolat teridentifikasi sebagai Kurthia gibsonii (kemiripan 100%).

Downloads

Published

2019-02-28

How to Cite

Mende, P. S., Pelealu, J., & Kolondam, B. (2019). IDENTIFIKASI MOLEKULER BAKTERI DALAM FESES KUCING (Felis domestica) YANG DITUMBUHKAN PADA DE MANN ROGOSA SHARPE AGAR (MRSA). PHARMACON, 8(1), 73–78. https://doi.org/10.35799/pha.8.2019.29239

Issue

Vol. 8 No. 1 (2019): PHARMACON

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)