IDENTIFIKASI KOMPONEN SENYAWA DALAM EKSTRAK N-HEKSANA UMBI RUMPUT TEKI (Cyperus rotundus L.) DENGAN ANALISIS GC-MS

Authors

  • Adinda Dwi Tania
  • Elly Juliana Suoth
  • Fatimawali Fatimawali
  • Trina Ekawati Tallei

DOI:

https://doi.org/10.35799/pha.10.2021.35600

Abstract

ABSTRACT

Nut grass (Cyperus rotundus L.) is a perennial weed which the tuber is used traditionally in treating several ailments. This study aimed to identify the phytochemical compounds from nut grass tuber. Extraction was carried out by maceration using n-hexane as solvent and then were analyzed with Gas Chromatography-Mass Spectrometry (GC-MS). The result showed that there were 48 peaks with 144 probable compounds detected in n-hexane extract of nut grass tuber. 7-Isopropenyl-1,4a-dimethyl-4,4a,5,6,7,8-hexahydro-3H-naphthalen-2-one; 1(2H)-Naphthalenone, 3,4,4a,5,6,7-hexahydro-4a,5-dimethyl-3-(1-methylethenyl)-, [3S-(3a,4aa,5a)]-; and 1,7-Dimethyl-4-(propan-2-ylidene)tricyclo[4.4.0.02,7]decan-3-one had the highest concentrations namely 43,92% in the extract. This study concluded that the compounds were classified as sesquiterpenoids which had various biological activities.

 

Keywords: N-hexane extract, GC-MS, chemical compounds, Nut Grass (Cyperus rotundus L.).

 

 

ABSTRAK

Rumput teki (Cyperus rotundus L.) adalah gulma perenial yang umbinya dimanfaatkan secara tradisional oleh masyarakat untuk mengobati beberapa jenis penyakit. Penelitian ini bertujuan untuk mengidentifikasi senyawa-senyawa fitokimia dari umbi rumput teki.  Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut n-heksana dan kemudian dilanjutkan dengan analisis Gas Chromatography-Mass Spectrometry (GC-MS). Hasil analisis menunjukkan terdapat 48 peak dengan 144 kemungkinan komponen senyawa yang terdeteksi dalam ekstrak n-heksana umbi rumput teki. Senyawa 7-Isopropenyl-1,4a-dimethyl-4,4a,5,6,7,8-hexahydro-3H-naphthalen-2-one; 1(2H)-Naphthalenone, 3,4,4a,5,6,7-hexahydro-4a,5-dimethyl-3-(1-methylethenyl)-, [3S-(3a,4aa,5a)]-; dan 1,7-Dimethyl-4-(propan-2-ylidene)tricyclo[4.4.0.02,7]decan-3-one memiliki persentase kadar tertinggi yaitu 43,92% dalam ekstrak. Berdasarkan hasil penelitian disimpulkan bahwa senyawa-senyawa ini termasuk dalam golongan seskuiterpenoid yang memiliki aktivitas biologis yang beragam.

 

Kata Kunci: Ekstrak n-heksana, GC-MS, komponen senyawa, Rumput Teki (Cyperus rotundus L.)

References

An, J.P., T.K.Q. Ha, H.W. Kim, B. Ryu, J. Kim, J. Park, C.H. Lee & W.K. Oh. 2019. Eudesmane Glycosides from Ambrosia artemisiifolia (Common Ragweed) as Potential Neuroprotective Agents. Journal of Natural Products. 82(5): 1128-1138.

Al-Snafi, A.E. 2016. A Review on Cyperus Rotundus: A Potential Medicinal Plant. IOSR Journal Of Pharmacy. 6(7): 32-48.

Al-Massarani, S., F. Al-Enzi1, M. Al-Tamimi, N. Al-Jomaiah, R. Al-Amri, K.H.C. BaÅŸer, N. Tabanca, A.S. Estep, J.J. Becnel, J.R. Bloomquist & B. Demirci. 2016. Composition & Biological Activity of Cyperus Rotundus L. Tuber Volatiles From Saudi Arabia. Natural Volatiles & Essential Oils. 3(2): 26-34.

Beattie, K.D., P.G. Waterman, P.I. Foster, D.R. Thompson & D.N. Leach. 2011. Chemical Composition and Cytotoxicity of Oils and Eremophilanes Derived from Various Parts of Eremophila mitchellii Benth. (Myoporaceae). Phytochemistry. 72(4-5): 400-408.

Beale, D.J., F.R. Pinu, K.A. Kouremenos, M.M. Poojary, V.K. Narayana, B.A. Boughton, K. Kanojia, S. Dayalan, O.A.H. Jones & D.A. Dias. 2018. Review of recent developments in GC–MS approaches to metabolomics-based research. Metabolomics. 14:152.

Bradfield, A.E., B.H. Hegde, B.S. Rao, J.L Simonsen & A.E. Gillam. 1936. 152. α-Cyperone, a Sesquiterpene Ketone from the Oil of Cyperus rotundus. Journal of the Chemical Society. 667–677.

Dunika, A.N.M., I.M.O.A. Parwata & I.A.M. Parthasutema. 2015. Analisis Kadar Metamfetamina Pada Sampel Darah dengan Metode GC-MS. Jurnal Chemistry Laboratory. 2(1): 18-29.

Hana, A. & K. Hifzul. 2018. Unani Perspective and New Researches of Sa’ad Ku’fi (Cyperus rotundus): A Review. Journal of Drug Delivery & Therapeutics. 8(6): 378-381.

Hariana, A. 2013. 262 Tumbuhan Obat & Khasiatnya. Penebar Swadaya, Jakarta.

Heldt, H.W., F. Heldt & B. Piechulla. 2005. Plant Biochemistry Third Edition. Elsevier Science, UK.

Hou, C., M. Kulka, J. Zhang, Y. Li & F. Guo. 2014. Occurrence and biological Activities of Eremophilane-type Sesquiterpenes. Mini-Reviews in Medicinal Chemistry. 14(8).

Hu, Q.P., X.M. Cao, D.L. Hao & L.L. Zhang. 2017. Chemical Composition, Antioxidant, DNA Damage Protective, Cytotoxic and Antibacterial Activities of Cyperus rotundus Rhizomes Essential Oil against Foodborne Pathogens. Scientific Report. 7(45231): 1-9.

Imam, H., Zarnigar, G. Sofi, S. Aziz & A. Lone. 2014. The Incredible Benefits of Nagarmotha (Cyperus rotundus). International Journal of Nutrition, Pharmacology, Neurological Disease. 4(1): 23-27.

Kamala, A., S.K Middha & C.S Karigar. 2018. Plants in Traditional Medicine with Special Reference to Cyperus rotundus L.: A Review. 3 Biotech. 8(7): 309-320.

Khan, S., R.J. Choi, D.U. Lee & Y.S. Kim. 2011. Sesquiterpene Derivatives Isolated from Cyperus rotundus L. Inhibit Inflammatory Signaling Mediated by NF-κB. Natural Product Science. 17(3): 250-255.

Kim, S., J. Chen, T. Cheng, A. Gindulyte, J. He, S. He, Q. Li, B.A. Shoemaker, P.A. Thiessen, B. Yu, L. Zaslavsky, J. Zhang & E.E. Bolton. 2019. PubChem in 2021: new data content and improved web interfaces. Nucleic acids research. 49(D1): D1388-D1395.

Kuete, V. 2013. Medicinal Plant Research in Africa. Elsevier, London.

Le Bideau, F., M. Kousara, L. Chen, L. Wei & F. Dumas. 2017. Tricyclic Sesquiterpenes from Marine Origin. Chemical Review. 117: 6110-6159.

Medeiros. 2018. Gas Chromatography – Mass Spectrometry (GC-MS). Encyclopedia of Geochemistry. 530-534.

Muthoharoh, H. & K. Hikmah. 2019. Analisis Kadar Flavonoid Total Ekstrak Umbi Rumput Teki (Cyperus Rotundus L.). Jurnal Ilmiah : J-HESTECH. 2(2): 127-132.

NIST Chemistry WebBook. 2018. https://webbook.nist.gov/chemistry/ [diakses tanggal 27 April 2021].

Nidugala, H., R. Avadhani, A. Prabhu, R. Basavaiah & K.N.S. Kumar. 2015. GC-MS Characterization of N-Hexane Soluble Compounds of Cyperus rotundus L. Rhizomes. Journal of Applied Pharmaceutical Science. 5(12): 096-100.

Nuryana, F.I., M.A. Chozin & D. Guntoro. 2019. High-performance Liquid Chromatography Analysis for α-cyperone and Nootkatone from the Tuber of Nutsedge (Cyperus rotundus L.) in the Tropics. Rasayan Journal of Chemistry. 12(1): 360-365.

Özek, G., E. Bedir, N. Tabanca, A. Ali, I.A. Khan, A. Duran, K.H.C. Başer & T. Özek. 2018. Isolation of eudesmane type sesquiterpene ketone from Prangos heyniae H.Duman & M.F.Watson essential oil and mosquitocidal activity of the essential oils. Open Chemistry. 16(1): 453-457.

Panomarenko, L.P., S.D. Ermolaeva, R.V. Doudkin, S.A. Dyshlovoy & P.G. Gorovoy. 2017. Eremophilane-type glucosides from the leaves of Ligularia calthifolia Maxim. Phytochemistry Letters. 21: 264-268.

Pei, X.D., H.L. Yao, L.Q. Shen, Y. Yang, L. Lu, J.S. Xiao, X.Y. Wang, Z.L. He & L.H. Jiang. 2020. α-Cyperone Inhibits the Proliferation of Human Cervical Cancer HeLa Cells via ROS-mediated PI3K/Akt/mTOR Signaling Pathway. European Journal of Pharmacology. 883: 173355.

Peng, B., R.F. Zhang, B.Z. Du, S.G. Jiao, X.C. Zhou, X.L. Gao, S.Y.L. Chen, P.F. Tu & X.Y. Chai. 2019. Alashanoids K-M, Bioactive Eremophilane Sesquiterpenoids from Syringa pinnatifolia. The Journal of Organic Chemistry. 86(10): 7263-7270.

Singab, A.B., F.S. Youssef, M.L. Ashour & M. Wink. 2013. The Genus Eremophila (Scrophulariaceae): An Ethnobotanical, Biological and Phytochemical review. Journal of Pharmacy and Pharmacology. 65: 1239-1279.

Sivapalan, S.R. 2013. Medicinal Uses and Pharmacological Activities of Cyperus rotundus Linn: A Review. International Journal of Scientific and Research Publications. 3(5): 467-476.

Tsankova, E & I. Ognyanov. 1976. Germazone, a Novel Tricyclic Sesquiterpene Ketone in The Essential Oil from Geranium macrorrhizum L. Tetrahedron Letters. 17(42): 3833-3836.

Wu, L., Z. Liao, C. Liu, H. Jia & J. Sun. 2016. Eremophilane Sesquiterpenes from the Genus Ligularia. Chemistry & Biodiversity. 13(6): 645-671.

Xu, H.B., Y.B. Ma, X.Y. Huang, C.A. Geng, H. Wang, Y. Zhao, T.H. Yang, X.L. Chen, C.Y. Yang, X.M. Zhang & J.J. Chen. 2015. Bioactivity-Guided Isolation of Anti-Hepatitis B Virus Active Sesquiterpenoids from The Traditional Chinese Medicine: Rhizomes of Cyperus rotundus. Journal of Etnopharmacology. 171: 131-140.

Yang, S., Z. Li, J. Wang, J. Ruan, C. Zheng, P. Huang, L. Han, Y. Zhang, & T. Wang. 2018. Eudesmane-Type Sesquiterpene Glycosides from Dictamnus dasycarpus Turcz. Molecules. 23(3): 642.

Yang, W., X. Chen, Y. Li, S. Guo, Z. Wang & X. Yu. 2020. Advances in Pharmacological Activities of Terpenoids. Natural Product Communications. 15(3): 1-13.

Yuyama, K.T., D. Fortkamp & W.R. Abraham. 2017. Eremophilane-type sesquiterpenes from fungi and their medicinal potential. Biology Chemistry. 399(1): 13-28.

Downloads

Published

2021-08-14

How to Cite

Tania, A. D., Suoth, E. J., Fatimawali, F., & Tallei, T. E. (2021). IDENTIFIKASI KOMPONEN SENYAWA DALAM EKSTRAK N-HEKSANA UMBI RUMPUT TEKI (Cyperus rotundus L.) DENGAN ANALISIS GC-MS. PHARMACON, 10(3), 975–984. https://doi.org/10.35799/pha.10.2021.35600

Issue

Vol. 10 No. 3 (2021): PHARMACON

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a  Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

  2. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)